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HAVCR1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150460

TIM-1 Antibody: The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is… More details

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Product Overview

Online Datasheet in PDF
Product Name
TIM-1 (HAVCR1), Polyclonal Antibody
Full Product Name
TIM-1 Antibody
Product Gene Name
Product Synonym Names
TIM-1; TIM; KIM1; TIM1; HAVCR; KIM-1; TIM-1; TIMD1; TIMD-1; HAVCR-1; Hepatitis A virus cellular receptor 1; Kidney injury molecule 1; HAVcr-1; hepatitis A virus cellular receptor 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Liquid
Purity/Purification
TIM-1 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
TIM-1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
TIM-1 peptide (MBS152715) is used for blocking the activity of TIM-1 antibody (MBS150460)
Molecular Weight
38,720 Da
OMIM
606518
3D Structure
ModBase 3D Structure for Q96D42
Conjugate
Unconjugated
Immunogen
TIM-1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human TIM-1.
Buffer
TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-HAVCR1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-HAVCR1 antibody
TIM-1 antibody can be used for the detection of TIM-1 by Western blot at 1 - 2 mug/mL. Knockdown (Knockout, KO validated) Antibody

Western Blot (WB) of anti-HAVCR1 antibody

Figure 1 Western Blot Validation in Human Cell Lines

Loading: 15 μg of lysates per lane.
Antibodies: TIM-1 3811 (1 μg /mL), 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Rabbit Anti-HAVCR1 Polyclonal Antibody – Cat# MBS150460

Western Blot (WB) of anti-HAVCR1 antibody

Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines

Loading: 15 μg of lysates per lane.
Antibodies: TIM-1 3809 (8 μg /mL), TIM-1 3811 (1 μg/mL), ?-actin (1 μg/mL), and GAPDH (0.02 μg/mL), overnight incubation at 4˚ C (3809) or 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Rabbit Anti-HAVCR1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS150460

Western Blot (WB) of anti-HAVCR1 antibody

Figure 3 Validation with TIM-1 siRNA Knockdown

HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2)
Loading: 15 μg of HeLa whole cell lysates per lane.
Antibodies: 3811 (1 μg /mL), 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Rabbit Anti-HAVCR1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS150460

Other Notes

Small volumes of anti-HAVCR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HAVCR1 purchase

MBS150460 is an antibody targeting HAVCR1. Also known as TIM-1 Antibody. Reactive in Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HAVCR1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
hepatitis A virus cellular receptor 1
NCBI Official Synonym Full Names
hepatitis A virus cellular receptor 1
NCBI Official Symbol
HAVCR1
NCBI Official Synonym Symbols
TIM; KIM1; TIM1; HAVCR; KIM-1; TIM-1; TIMD1; TIMD-1; HAVCR-1
UniProt Protein Name
Hepatitis A virus cellular receptor 1
UniProt Synonym Protein Names
Kidney injury molecule 1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIMD-1; T-cell immunoglobulin mucin receptor 1; TIM; TIM-1; T-cell membrane protein 1
UniProt Gene Name
HAVCR1
UniProt Synonym Gene Names
KIM1; TIM1; TIMD1; HAVcr-1; KIM-1; TIMD-1; TIM; TIM-1
UniProt Entry Name
HAVR1_HUMAN
NCBI Protein Information
hepatitis A virus cellular receptor 1; kidney injury molecule 1; T-cell membrane protein 1; T-cell immunoglobulin mucin receptor 1; T-cell immunoglobulin mucin family member 1; T cell immunoglobin domain and mucin domain protein 1

NCBI Summary for HAVCR1

The protein encoded by this gene is a membrane receptor for both human hepatitis A virus (HHAV) and TIMD4. The encoded protein may be involved in the moderation of asthma and allergic diseases. The reference genome represents an allele that retains a MTTVP amino acid segment that confers protection against atopy in HHAV seropositive individuals. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]
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UniProt Comments for HAVCR1

TIM-1: May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. Belongs to the immunoglobulin superfamily. TIM family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q33.2

Cellular Component: integral to membrane

Molecular Function: viral receptor activity

Biological Process: entry of virus into host cell

Disease: Ige Responsiveness, Atopic
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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