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PCDH12 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150881

PCDH12 Antibody: Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 12 (PCDH12) was initially identified through PCR screening of mouse heart microvascular endothelial cell… More details

$530 / 0.1 mg +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PCDH12, Polyclonal Antibody
Full Product Name
PCDH12 Antibody
Product Gene Name
Product Synonym Names
PCDH12; VECAD2; VE-cadherin-2; Protocadherin-12; Vascular cadherin-2; VE-cad-2; protocadherin 12
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
PCDH12 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
This antibody is predicted to not cross-react with PCDH18.
Preparation and Storage
PCDH12 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
PCDH12 peptide (MBS152678) is used for blocking the activity of PCDH12 antibody (MBS150881)
Molecular Weight
128,995 Da
OMIM
605622
3D Structure
ModBase 3D Structure for Q9NPG4
Conjugate
Unconjugated
Immunogen
PCDH12 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH12.
Buffer
PCDH12 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-PCDH12 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PCDH12 antibody
PCDH12 antibody can be used for detection of PCDH12 by Western blot at 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.

Western Blot (WB) of anti-PCDH12 antibody

Western blot analysis of PCDH12 in K562 cell lysate with PCDH12 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-PCDH12 Polyclonal Antibody – Cat# MBS150881

Immunohistochemistry (IHC) of anti-PCDH12 antibody

Immunohistochemistry of PCDH12 in rat lung tissue with PCDH12 antibody at 5 μg/mL.
Rabbit Anti-PCDH12 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150881

Other Notes

Small volumes of anti-PCDH12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PCDH12 purchase

MBS150881 is an antibody targeting PCDH12. Also known as PCDH12 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PCDH12. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protocadherin-12
NCBI Official Synonym Full Names
protocadherin 12
NCBI Official Symbol
PCDH12
NCBI Official Synonym Symbols
VECAD2; VE-cadherin-2
UniProt Protein Name
Protocadherin-12
UniProt Synonym Protein Names
Vascular cadherin-2; Vascular endothelial cadherin-2; VE-cad-2; VE-cadherin-2
Protein Family
UniProt Gene Name
PCDH12
UniProt Synonym Gene Names
VE-cad-2; VE-cadherin-2
UniProt Entry Name
PCD12_HUMAN
NCBI Protein Information
protocadherin-12; VE-cad-2; VE-cadherin 2; vascular cadherin-2; vascular endothelial cadherin 2; vascular endothelial cadherin-2

NCBI Summary for PCDH12

This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The encoded protein consists of an extracellular domain containing 6 cadherin repeats, a transmembrane domain and a cytoplasmic tail that differs from those of the classical cadherins. The gene localizes to the region on chromosome 5 where the protocadherin gene clusters reside. The exon organization of this transcript is similar to that of the gene cluster transcripts, notably the first large exon, but no significant sequence homology exists. The function of this cellular adhesion protein is undetermined but mouse protocadherin 12 does not bind catenins and appears to have no affect on cell migration or growth. [provided by RefSeq, Jul 2008]
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UniProt Comments for PCDH12

PCDH12: Cellular adhesion molecule that may play an important role in cell-cell interactions at interendothelial junctions. Promotes homotypic calcium-dependent aggregation and adhesion and clusters at intercellular junctions. Unable to bind to catenins, weakly associates with the cytoskeleton.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: integral to plasma membrane; plasma membrane; intercellular junction

Molecular Function: calcium ion binding

Biological Process: glycogen metabolic process; calcium-dependent cell-cell adhesion; neuron recognition; homophilic cell adhesion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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