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GFR Alpha 3 (GFRA3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS151276

GFR alpha 3 Antibody: Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GFR alpha 3 (Gfra3), Polyclonal Antibody
Full Product Name
GFR alpha 3 Antibody
Product Gene Name
Product Synonym Names
GFR alpha 3; Y15110; GFRalpha3; GDNF family receptor alpha-3; GDNF receptor alpha-3; glial cell line derived neurotrophic factor family receptor alpha 3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
GFR alpha 3 Antibody is Antibody is DEAE purified.
Preparation and Storage
GFR alpha 3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
Molecular Weight
44,307 Da
3D Structure
ModBase 3D Structure for O35118
Conjugate
Unconjugated
Immunogen
GFR alpha 3 antibody was raised against a 14 amino acid peptide near the center of murine GFR alpha 3.
Buffer
GFR alpha 3 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-Gfra3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Gfra3 antibody
GFR alpha 3 antibody can be used for detection of GFR alpha 3 expression by Western blot at 0.5 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL.

Western Blot (WB) of anti-Gfra3 antibody

Western blot analysis of GFR alpha 3 in crude cell membrane fractions of mouse heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with GFR alpha 3 antibody at 1 2 μg/mL.
Rabbit Anti-GFRA3 Polyclonal Antibody – Cat# MBS151276

Immunohistochemistry (IHC) of anti-Gfra3 antibody

Immunohistochemistry of GFR alpha 3 in mouse heart tissue with GFR alpha 3 antibody at 10 μg/mL.
Rabbit Anti-GFRA3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS151276

Immunofluorescence (IF) of anti-Gfra3 antibody

Immunofluorescence of GFR alpha 3 in mouse kidney tissue with GFR alpha 3 antibody at 5 μg/ml.

Green: GFR alpha 3 antibody (1137)
Red: Phylloidin staining
Blue: DAPI staining
Rabbit Anti-GFRA3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS151276

Immunohistochemistry (IHC) of anti-Gfra3 antibody

Immunohistochemistry of GFR alpha 3 in mouse kidney tissue with GFR alpha 3 antibody at 5 μg/ml.
Rabbit Anti-GFRA3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS151276

Other Notes

Small volumes of anti-Gfra3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Gfra3 purchase

MBS151276 is an antibody targeting Gfra3. Also known as GFR alpha 3 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Gfra3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Mus musculus mRNA for GFRalpha-3, complete cds
NCBI Official Synonym Full Names
glial cell line derived neurotrophic factor family receptor alpha 3
NCBI Official Symbol
Gfra3
NCBI Official Synonym Symbols
Y15110; GFRalpha3
UniProt Protein Name
GDNF family receptor alpha-3
Protein Family
UniProt Gene Name
Gfra3
UniProt Synonym Gene Names
GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3
UniProt Entry Name
GFRA3_MOUSE
NCBI Protein Information
GDNF family receptor alpha-3; GFR alpha-3; GFR-alpha-3; GDNFR-alpha-3; GDNF receptor alpha-3

NCBI Summary for Gfra3

This gene encodes a cell surface glycoprotein and member of the glial cell line-derived neurotrophic receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein mediates binding of the ligand artemin to the ret receptor tyrosine kinase, and this interaction may regulate thermal pain and axon regeneration. Homozygous knockout mice for this gene exhibit impaired proliferation of cultured neuroblasts and impaired development of the superior cervical ganglion. [provided by RefSeq, Aug 2015]
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UniProt Comments for Gfra3

GFRA3: Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; coreceptor activity; receptor activity; axon guidance receptor activity

Biological Process: axon guidance; sympathetic nervous system development; neuron migration; neuron development; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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