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Il-36b (IL36B) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS153331

IL-36B is is a member of the interleukin 1 cytokine family whose gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (1). IL-36B is thought to activate the NF-kappaB pathway through IL-1 receptor family members IL-1RL2… More details

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Product Overview

Online Datasheet in PDF
Product Name
IL-36B (IL36B), Polyclonal Antibody
Full Product Name
IL-36B Antibody
Product Gene Name
Product Synonym Names
IL-36B; FIL1; FIL1H; IL1F8; IL1H2; IL-1F8; IL-1H2; IL1-ETA; FIL1-(ETA); FILI-(ETA); Interleukin-36 beta; FIL1 eta; IL-1 eta; interleukin 36, beta
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid ; IL-36B antibody is supplied in PBS containing 0.02% sodium azide.
Purity/Purification
IL-36B antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
IL-36B antibody is human specific. At least two isoforms of IL-36B are known to exist; this antibody will only detect the longer isoform. IL-36B antibody will not cross-react with IL-36A or IL-36G.
Preparation and Storage
Antibody can be stored at 4 degree C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
IL-36B peptide (MBS153830) is used for blocking the activity of IL-36B antibody (MBS153331)
Molecular Weight
Predicted: 18 kDa
Observed: 17 kDa
OMIM
605508
3D Structure
ModBase 3D Structure for Q9NZH7
Conjugate
Unconjugated
Immunogen
IL-36B antibody was raised against a 16 amino acid peptide near the carboxy terminus of human IL-36B. The immunogen is located within the last 50 amino acids of IL-36B.
Applications Tested/Suitable for anti-IL36B antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-IL36B antibody
Optimal dilutions for each application to be determined by the researcher.

Western Blot (WB) of anti-IL36B antibody

Western blot analysis of IL-36B in A549 cell lysate with IL-36B antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-IL36B Polyclonal Antibody – Cat# MBS153331

Immunohistochemistry (IHC) of anti-IL36B antibody

Immunohistochemistry of IL-36B in human lung tissue with IL-36B antibody at 2.5 μg/mL.
Rabbit Anti-IL36B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS153331

Immunofluorescence (IF) of anti-IL36B antibody

Immunofluorescence of IL-36B in human lung tissue with IL-36B antibody at 20 μg/mL.
Rabbit Anti-IL36B Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS153331

Other Notes

Small volumes of anti-IL36B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL36B purchase

MBS153331 is an antibody targeting IL36B. Also known as IL-36B Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IL36B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interleukin-36 beta isoform 1
NCBI Official Synonym Full Names
interleukin 36, beta
NCBI Official Symbol
IL36B
NCBI Official Synonym Symbols
FIL1; FIL1H; IL1F8; IL1H2; IL-1F8; IL-1H2; IL1-ETA; FIL1-(ETA); FILI-(ETA)
UniProt Protein Name
Interleukin-36 beta
UniProt Synonym Protein Names
FIL1 eta; Interleukin-1 eta; IL-1 eta; Interleukin-1 family member 8; IL-1F8; Interleukin-1 homolog 2; IL-1H2
Protein Family
UniProt Gene Name
IL36B
UniProt Synonym Gene Names
IL1F8; IL1H2; IL-1 eta; IL-1F8; IL-1H2
UniProt Entry Name
IL36B_HUMAN
NCBI Protein Information
interleukin-36 beta; FIL1 eta; IL-1 eta; IL-1F8 (FIL1-eta); interleukin-1 eta; interleukin 1, eta; interleukin-1 homolog 2; Interleukin-1 Superfamily e; family of interleukin 1-eta; interleukin-1 family member 8; IL1F8 (Canonical product IL-1F8a); interleukin 1 family, member 8 (eta)

NCBI Summary for IL36B

The protein encoded by this gene is a member of the interleukin 1 cytokine family. Protein structure modeling indicated that this cytokine may contain a 12-stranded beta-trefoil structure that is conserved between IL1A (IL-A alpha) and IL1B (IL-1 beta). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
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UniProt Comments for IL36B

IL36B: Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensins 4 and 103 as well as a number of matrix metalloproteases. Belongs to the IL-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-1 receptor binding; cytokine activity

Biological Process: positive regulation of T cell differentiation; cytokine and chemokine mediated signaling pathway; positive regulation of interleukin-6 production; innate immune response; immune response; inflammatory response
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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