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ARHGAP25 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS830854

MBS830854 is an antibody targeting ARHGAP25. Also known as ARHGAP25 antibody. Host: Mouse. Mouse monoclonal ARHGAP25 antibody More details

$1615 / 0.1 mL +2 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ARHGAP25, Monoclonal Antibody
Full Product Name
ARHGAP25 antibody
Product Gene Name
Product Synonym Names
Monoclonal ARHGAP25; Anti-ARHGAP25; Rho Gtpase Activating Protein 25; KAIA0053; ARHGAP25; ARHGAP 25; ARHGAP-25
Clonality
Monoclonal
Isotype
IgG1
Clone Number
11B9
Host
Mouse
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purity/Purification
ARHGAP25 antibody was purified by affinity chromatography.
Concentration
500 ug-1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
72,502 Da
OMIM
610587
3D Structure
ModBase 3D Structure for P42331
Cross-Reactivity
Human
Immunogen
ARHGAP25 antibody was raised in mouse using a full length recombinant protein of human ARHGAP25 (NP_055697) produced in HEK293T cells, as the immunogen.

Product Categories/Family for anti-ARHGAP25 antibody

Applications Tested/Suitable for anti-ARHGAP25 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-ARHGAP25 antibody
FC: 1:100
IF: 1:100
IHC: 1:150
WB: 1:2000

Western Blot (WB) of anti-ARHGAP25 antibody

Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ARHGAP25 protein (Right) or empty vector (Left) detected with ARHGAP25 antibody
Mouse Anti-ARHGAP25 Monoclonal Antibody – Cat# MBS830854

Immunohistochemistry (IHC) of anti-ARHGAP25 antibody

Immunohistochemical analysis of ARHGAP25 protein in paraffin embedded Carcinoma of Human lung tissue using ARHGAP25 antibody
Mouse Anti-ARHGAP25 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS830854

Immunofluorescence (IF) of anti-ARHGAP25 antibody

Immunofluorescent staining of COS7 cells transiently transfected with recombinant ARHGAP25 protein using ARHGAP25 antibody
Mouse Anti-ARHGAP25 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS830854

Other Notes

Small volumes of anti-ARHGAP25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ARHGAP25. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
rho GTPase-activating protein 25 isoform b
NCBI Official Synonym Full Names
Rho GTPase activating protein 25
NCBI Official Symbol
ARHGAP25
NCBI Official Synonym Symbols
KAIA0053; HEL-S-308
UniProt Protein Name
Rho GTPase-activating protein 25
UniProt Synonym Protein Names
Rho-type GTPase-activating protein 25
UniProt Gene Name
ARHGAP25
UniProt Synonym Gene Names
KIAA0053
UniProt Entry Name
RHG25_HUMAN
NCBI Protein Information
rho GTPase-activating protein 25

NCBI Summary for ARHGAP25

ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008]

UniProt Comments for ARHGAP25

ARHGAP25: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, Rac/Rho; GAPs

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: cytosol

Molecular Function: GTPase activator activity

Biological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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