• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ARF6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS153602

The ADP-ribosylation factor 6 (ARF6) is a member of the ARF family, which is part of the RAS superfamily (1). The ARF proteins are small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ARF6, Polyclonal Antibody
Full Product Name
ARF6 Antibody
Product Gene Name
anti-ARF6 antibody
Product Synonym Names
ARF6; ADP-ribosylation factor 6; ADP-ribosylation factor 6
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
ARF6 antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
ARF6 antibody is human, mouse and rat reactive.
Preparation and Storage
ARF6 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Antibody/Peptide Pairs
ARF6 peptide (MBS153994) is used for blocking the activity of ARF6 antibody (MBS153602)
Molecular Weight
Predicted: 19 kDa
Observed: 18 kDa
OMIM
600464
3D Structure
ModBase 3D Structure for P62330
Conjugate
Unconjugated
Buffer
ARF6 antibody is supplied in PBS containing 0.02% sodium azide.
Homology
Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%)
Immunogen
ARF6 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ARF6. The immunogen is located within the last 50 amino acids of ARF6.
Positive Control
1) MBS151657 - Rat Liver Tissue Lysate
2) MBS154338 - Human Kidney Tissue Slide
Applications Tested/Suitable for anti-ARF6 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-ARF6 antibody
ARF6 antibody can be used for detection of ARF6 by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Western Blot (WB) of anti-ARF6 antibody

Western blot analysis of ARF6 in rat liver tissue lysate with ARF6 antibody at 1 μg/ml.
Rabbit Anti-ARF6 Polyclonal Antibody – Cat# MBS153602

Immunohistochemistry (IHC) of anti-ARF6 antibody

Immunohistochemistry of ARF6 in human kidney tissue with ARF6 antibody at 5 μg/mL.
Rabbit Anti-ARF6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS153602

Immunofluorescence (IF) of anti-ARF6 antibody

Immunofluorescence of ARF6 in human kidney tissue with ARF6 antibody at 20 μg/mL.
Rabbit Anti-ARF6 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS153602

Other Notes

Small volumes of anti-ARF6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ARF6 purchase

MBS153602 is an antibody targeting ARF6. Also known as ARF6 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ARF6. It may not necessarily be applicable to this product.

NCBI GI #
4502211
NCBI GeneID
382
NCBI Accession #
NP_001654
UniProt Primary Accession #
P62330
UniProt Secondary Accession #
P26438; Q6FGZ2
UniProt Related Accession #
P62330
GenBank Nucleotide #
NM_001663.3
NCBI Official Full Name
ADP-ribosylation factor 6
NCBI Official Synonym Full Names
ADP-ribosylation factor 6
NCBI Official Symbol
ARF6
UniProt Protein Name
ADP-ribosylation factor 6
Protein Family
ADP-ribosylation factor
UniProt Gene Name
ARF6
UniProt Entry Name
ARF6_HUMAN
NCBI Protein Information
ADP-ribosylation factor 6

NCBI Summary for ARF6

This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for ARF6

ARF6: GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development. Contributes to the regulation of dendritic branching and filopodia extension. Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA. Interacts with NCS1/FREQ at the plasma membrane. Interacts with RAB11FIP3 and RAB11FIP4. Interacts with USP6 (via Rab-GAP TBC domain). Interacts with ECM29. Interacts with TBC1D24. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein, monomeric; G protein, monomeric, ARF; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q21.3

Cellular Component: ruffle; Golgi apparatus; filopodium membrane; focal adhesion; membrane; endocytic vesicle; recycling endosome membrane; early endosome; plasma membrane; cell cortex; midbody; endosome; cleavage furrow

Molecular Function: GTPase activity; protein binding; GTP binding; thioesterase binding

Biological Process: myeloid cell apoptosis; metabolic process; regulation of filopodium formation; cell cycle; liver development; vesicle-mediated transport; regulation of Rac protein signal transduction; protein transport; positive regulation of actin filament polymerization; cell division; small GTPase mediated signal transduction; ruffle organization and biogenesis; negative regulation of receptor-mediated endocytosis; cortical actin cytoskeleton organization and biogenesis; cell motility; cell adhesion
Show more

Research Articles on ARF6

1. EFA6 is regulated by a negative feedback loop, which is mediated by an allosteric interaction of Arf6-GTP with a domain of EFA6 and monitors the activation of Arf1 and Arf6 differentially

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ARF6 antibody

 Reference Product  PubMed Publications
 RAC1 antibody  >75 publications with ARF6 and RAC1
 PLD1 antibody  >21 publications with ARF6 and PLD1
 ACAP1 antibody  >13 publications with ARF6 and ACAP1
 GGA3 antibody  >7 publications with ARF6 and GGA3
 ASAP3 antibody  >6 publications with ARF6 and ASAP3
 CYTH2 antibody  >3 publications with ARF6 and CYTH2
 SPAG9 antibody  >3 publications with ARF6 and SPAG9

Pathways associated with anti-ARF6 antibody

 Products by Pathway  Pathway Diagram
 Arf6 Downstream Pathway antibodies  Arf6 Downstream Pathway Diagram
 Arf6 Signaling Events Pathway antibodies  Arf6 Signaling Events Pathway Diagram
 Arf6 Trafficking Events Pathway antibodies  Arf6 Trafficking Events Pathway Diagram
 Class I PI3K Signaling Events Pathway antibodies  Class I PI3K Signaling Events Pathway Diagram
 E-cadherin Signaling In The Nascent Adherens Junction Pathway antibodies  E-cadherin Signaling In The Nascent Adherens Junction Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram

Diseases associated with anti-ARF6 antibody

 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >18 publications with ARF6 and Neoplasm Metastasis
 Lung Neoplasms Antibodies  >7 publications with ARF6 and Lung Neoplasms
 Inflammation Antibodies  >7 publications with ARF6 and Inflammation
 Disease Models, Animal Antibodies  >5 publications with ARF6 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >5 publications with ARF6 and Neoplasms, Experimental
 Embryo Loss Antibodies  >3 publications with ARF6 and Embryo Loss
 Carcinoma, Hepatocellular Antibodies  >3 publications with ARF6 and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >3 publications with ARF6 and Liver Diseases
 Kidney Diseases Antibodies  >2 publications with ARF6 and Kidney Diseases
 Necrosis Antibodies  >2 publications with ARF6 and Necrosis

Organs/Tissues associated with anti-ARF6 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >65 publications with ARF6 and Blood
 Brain Antibodies  >64 publications with ARF6 and Brain
 Nerve Antibodies  >51 publications with ARF6 and Nerve
 Kidney Antibodies  >41 publications with ARF6 and Kidney
 Lung Antibodies  >31 publications with ARF6 and Lung
 Heart Antibodies  >29 publications with ARF6 and Heart
 Muscle Antibodies  >24 publications with ARF6 and Muscle
 Vascular Antibodies  >21 publications with ARF6 and Vascular
 Embryonic Tissue Antibodies  >14 publications with ARF6 and Embryonic Tissue
 Ovary Antibodies  >12 publications with ARF6 and Ovary
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000