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MAP2K1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS154381

The mitogen-activated protein kinase kinase 1 (MAP2K1, also known as MEK1) and the highly homologous MEK2 are members of the dual specificity protein kinase family (1). MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an… More details

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Product Overview

Online Datasheet in PDF
Product Name
MEK1/2 (MAP2K1), Polyclonal Antibody
Full Product Name
MEK1/2 Antibody
Product Gene Name
Product Synonym Names
Mitogen-activated protein kinase kinase 1, MAP2K1, MAPKK1, MEK1, MKK1, PRKMK1, CFC3, Mitogen-activated protein kinase kinase 2, MAP2K2, MAPKK2, MEK2, MKK2, PRKMK2, CFC
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
MEK1/2 antibody is human, mouse, and rat reactive. This antibody detects both MEK1 and MEK2.
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
Antibody/Peptide Pairs
MEK1/2 peptide (MBS154419) is used for blocking the activity of MEK1/2 antibody (MBS154381)
Molecular Weight
Predicted: 43 kDa
Observed: 44 kDa
3D Structure
ModBase 3D Structure for Q0VD16
Immunogen
MEK1/2 antibody was raised against a 15 amino acid peptide near the center of human MEK1.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
Human Brain Tissue Lysate
Conjugate
Unconjugated

Product Categories/Family for anti-MAP2K1 antibody

Applications Tested/Suitable for anti-MAP2K1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-MAP2K1 antibody
MEK1/2 antibody can be used for detection of MEK1/2 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-MAP2K1 antibody

Western blot analysis of MEK1/2 in human brain tissue lysate with MEK1/2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-MAP2K1 Polyclonal Antibody – Cat# MBS154381

Immunohistochemistry (IHC) of anti-MAP2K1 antibody

Immunohistochemistry of MEK1/2 in mouse brain tissue with MEK1/2 antibody at 5 μg/mL.
Rabbit Anti-MAP2K1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS154381

Immunofluorescence (IF) of anti-MAP2K1 antibody

Immunofluorescence of MEK1/2 in mouse brain tissue with MEK1/2 antibody at 20 μg/mL.
Rabbit Anti-MAP2K1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS154381

Product References and Citations for anti-MAP2K1 antibody

Zheng CF and Guan KL. Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2. J. Biol. Chem. 1993; 268:11435-9. Marais R and Marshall CJ. Control of the ERK MAP kinase cascade by Ras and Raf. Cancer Surv. 1996; 27:101-25.

Other Notes

Small volumes of anti-MAP2K1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAP2K1 purchase

MBS154381 is an antibody targeting MAP2K1. Also known as MEK1/2 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP2K1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
dual specificity mitogen-activated protein kinase kinase 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase 1
NCBI Official Symbol
MAP2K1
NCBI Official Synonym Symbols
CFC3; MEK1; MKK1; MAPKK1; PRKMK1
UniProt Protein Name
MAP2K1 protein
UniProt Synonym Protein Names
MAP2K1 protein
UniProt Gene Name
MAP2K1
UniProt Entry Name
Q0VD16_BOVIN
NCBI Protein Information
dual specificity mitogen-activated protein kinase kinase 1

NCBI Summary for MAP2K1

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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