COVID 19 Spike RBD Coronavirus (COVID-19) ELISA Kit

Intended Uses: SARS-CoV-2 (2019-nCoV) RBD ELISA Kit is a sandwich solid phase ELISA designed to measure RBD protein in bronchoalveolar lavage fluid and nasopharyngeal swab samples. The microplate provided in this kit has been pre-coated with RBD monoclonal antibody. Standards or samples are then added to the appropriate wells with a biotin-conjugated antibody specific to RBD. Next, Streptavidin Horseradish Peroxidase (SA-HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RBD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The concentration of RBD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of COVID-19 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS157728 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the COVID 19 Spike RBD Coronavirus (COVID-19) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COVID-19. The ELISA analytical biochemical technique of the MBS157728 kit is based on COVID-19 antibody-COVID-19 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COVID-19 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COVID-19. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).