6-Phosphofructokinase, Liver Type (PFKL) ELISA Kit for Mouse

PFKL: phosphofructokinase, liver type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Two alternatively spliced isoforms have been described.

Protein type: Carbohydrate Metabolism - fructose and mannose; EC 2.7.1.11; Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; Carbohydrate Metabolism - galactose

Cellular Component: 6-phosphofructokinase complex; membrane; cytoplasm; cytosol

Molecular Function: transferase activity; monosaccharide binding; identical protein binding; metal ion binding; nucleotide binding; kinase activity; catalytic activity; kinase binding; 6-phosphofructokinase activity; ATP binding

Biological Process: fructose 1,6-bisphosphate metabolic process; glycolysis; metabolic process; carbohydrate phosphorylation; carbohydrate metabolic process; response to glucose stimulus; fructose 6-phosphate metabolic process; phosphorylation; protein oligomerization; protein homotetramerization; negative regulation of insulin secretion