Gamma H2A Histone Family, Member X (gamma-H2AX) ELISA Kit for Human

Principle of the Assay: H2AFX ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-H2AFX antibody and an H2AFX-HRP conjugate. The assay sample and buffer are incubated together with H2AFX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the H2AFX concentration since H2AFX from samples and H2AFX-HRP conjugate compete for the anti-H2AFX antibody binding site. Since the number of sites is limited, as more sites are occupied by H2AFX from the sample, fewer sites are left to bind H2AFX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The H2AFX concentration in each sample is interpolated from this standard curve.

Intended Uses: This H2AFX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human H2AFX. This ELISA kit for research use only, not for therapeutic or test applications!

Small volumes of gamma-H2AX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7254195 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the gamma H2A Histone Family, Member X (gamma-H2AX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing gamma-H2AX. The ELISA analytical biochemical technique of the MBS7254195 kit is based on gamma-H2AX antibody-gamma-H2AX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect gamma-H2AX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, gamma-H2AX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).