Oxiselect Oxidative RNA Damage (8-OHG Quantitation) ELISA Kit

Principle of the Assay: The Oxidative RNA Damage ELISA kit is a competitive ELISA for the quantitative measurement of 8-OHG. The unknown 8-OHG samples or 8-OHG standards are first added to an 8-OHG/BSA conjugate preabsorbed EIA plate. After a brief incubation, an anti-8-OHG monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The 8-OHG content in unknown samples is determined by comparison with predetermined 8-OHG standard curve.!!Background/Introduction: Free radicals and other reactive species are constantly generated in vivo and cause oxidative damage to biomolecules, a process held in check only by the existence of multiple antioxidant and repair systems as well as the replacement of damaged nuclei acids, proteins and lipids. RNA and DNA are probably the most biologically significant targets of oxidative attack. Recently, oxidative damage to RNA molecules has been described in several neurological diseases including Alzheimer's disease, Parkinson's disease, Down syndrome, dementia with Lewy bodies, prion disease, subacute sclerosing panencephalitis, and xeroderma pigmentosum. Among numerous types of RNA oxidative damage, the formation of 8-hydroxyguanosine (8-OHG) is a ubiquitous marker of oxidative stress. The OxiSelect™ Oxidative RNA Damage ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 8-OHG in urine, serum, cerebrospinal fluid or other cell or tissue RNA samples. The quantity of 8-OHG in unknown sample is determined by comparing its absorbance with that of a known 8-OHG standard curve. The kit has an 8-OHG detection sensitivity range of 300 pg/mL to 40 ng/mL. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.

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Small volumes of OxiSelect Oxidative RNA Damage (8-OHG Quantitation) elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168447 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the OxiSelect Oxidative RNA Damage (8-OHG Quantitation) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OxiSelect Oxidative RNA Damage (8-OHG Quantitation). The ELISA analytical biochemical technique of the MBS168447 kit is based on OxiSelect Oxidative RNA Damage (8-OHG Quantitation) antibody-OxiSelect Oxidative RNA Damage (8-OHG Quantitation) antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OxiSelect Oxidative RNA Damage (8-OHG Quantitation) antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OxiSelect Oxidative RNA Damage (8-OHG Quantitation). Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).