GFP ELISA Kit

Background: Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from the pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can fuse with proteins of interest without interfering significantly with their assembly and function. Based on the structure of the GFP molecule, many GFP variants have been created with much improved fluorescence emission, or shifted excitation or emission spectra that are well suited for fluorescence microscopy and flow cytometry. Although GFP expression can be easily detected under a fluorescence microscope, GFP fluorescence intensity varies from cell to cell because of the heterogeneity nature of GFP expression. In order to quantitate the GFP expression in cells, FACS analysis is usually required, which is both expensive and time consuming. GFP ELISA Kit is an enzyme immunoassay developed for detection and quantitation of GFP or GFP fusion protein in cell or tissue samples. The quantity of GFP or its variants (including BFP, CFP and YFP) in an unknown sample is determined by comparing its absorbance with that of a known recombinant GFP standard curve. The kit has detection sensitivity limit of 30 pg/mL GFP. The kit also provides an efficient system for rapid quantitation of GFP lentivirus titer for both viral supernatant and purified virus. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and GFP samples.

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Small volumes of GFP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168721 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GFP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GFP. The ELISA analytical biochemical technique of the MBS168721 kit is based on GFP antibody-GFP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GFP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GFP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).