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Aspartate Aminotransferase (GOT1) Porcine Active Protein

For Research Use Only. Not for use in diagnostic procedures. Popular Catalog #: MBS173082

This GOT1 active protein is porcine heart aspartate aminotransferase (AST), supplied as an enzymatically active preparation for liver enzyme assay development and diagnostic calibration research. The lab produces Porcine Heart Aspartate Transferase (AST),… More details

$250 / 5000 Units +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Aspartate Aminotransferase (GOT1), Active Protein
Full Product Name
Glutamate Oxaloacetate Transmainase
Product Gene Name
Product Synonym Names
GOT, Aspartate Transaminase; AST
Form/Format
Powder, lyophilized from sodium phosphate, pH7.0 with pyridoxal-5-phosphate
Preparation and Storage
Store at -20 degree C in lyophilized form, protect from mositure. Aliquot and freeze at -20 degree C after reconstitution.
3D Structure
ModBase 3D Structure for P00503
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
EC Number
2.6.1.1
CAS Number
9000-97-9
Source
Porcine heart
Protein
0.64 mg protein/mg solid (Coomassie), 1.13 mg protein/mg sollid (A280)
Activity
387 U/mg (Dimension Clinical Chemistry System)
Unit Definition
One unit will catalyze the transamination of one miromole of L-aspartate to (ALPHA)-ketoglutarate forming L-glutamate and oxaloacetate per minute at 37 degree C and pH 7.8
Specific Activity
610 U/mg protein (Coomasie), 340 U/mg protein (A 280)
Appearance
Yellow to yellow-green powder
Contaminants
CPK: <0.1%
GLDH: <0.1%
GPT/ALT: <0.1%
LDH: <0.1%
MDH: <0.01%
Protease: <0.1% (Equivalent to <0.001 O.D /8 min)
Ammonia
<0.005 micromole/mg (<0.01%)
Reconstitution
> 1 mg/ml in tris buffered saline, 1% BSA, pH 8.0
Handling
Handle as a potentially hazardous substance.

Other Notes

Small volumes of GOT1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GOT1 purchase

MBS173082 is an active protein targeting GOT1. Also known as Glutamate Oxaloacetate Transmainase.

Additional Details

NCBI/Uniprot data below describe general gene information for GOT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Aspartate aminotransferase, cytoplasmic
NCBI Official Symbol
GOT1
NCBI Official Synonym Symbols
cCAT; CASPAT
UniProt Protein Name
Aspartate aminotransferase, cytoplasmic
UniProt Synonym Protein Names
Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A
UniProt Gene Name
GOT1
UniProt Synonym Gene Names
cAspAT; cCAT
UniProt Entry Name
AATC_PIG
NCBI Protein Information
aspartate aminotransferase, cytoplasmic; transaminase A; cysteine transaminase, cytoplasmic; glutamate oxaloacetate transaminase 1; cysteine aminotransferase, cytoplasmic

UniProt Comments for GOT1

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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