Rabbit Anti-RPS20 Monoclonal Antibody – Cat# MBS1750082

Western blot analysis of RPS20 expression in HeLa cell lysate (MBS1750082).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS20 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPS20

RPS20 Antibody – Rabbit Monoclonal

Name: RPS20 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750082
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750082

MBS1750082 is an antibody targeting RPS20. Also known as Anti-RPS20/Ribosomal Protein S20 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RPS20/Ribosomal Protein S20, Monoclonal Antibody

Full Product Name

Anti-RPS20/Ribosomal Protein S20 Rabbit Monoclonal Antibody

Product Gene Name

anti-RPS20 antibody

Product Synonym Names

40S ribosomal protein S20; RPS20

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

13373 MW

OMIM

L06498 mRNA

3D Structure

ModBase 3D Structure for P60866

Immunogen

A synthesized peptide derived from human RPS20

Subcellular Localization

Cytoplasm.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-RPS20 antibody

Epigenetics and Nuclear Signaling; DNA/RNA; Translation; Ribosome

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-RPS20 antibody

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-RPS20 antibody

WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200

Other Notes

Small volumes of anti-RPS20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RPS20. It may not necessarily be applicable to this product.

NCBI GI #

4506697

NCBI GeneID

6224

NCBI Accession #

NP_001014.1

UniProt Primary Accession #

P60866

UniProt Secondary Accession #

P17075; Q5M8S9; B2R4F4; B4DW28

UniProt Related Accession #

P60866

GenBank Nucleotide #

NM_001023.3

NCBI Official Full Name

40S ribosomal protein S20 isoform 2

NCBI Official Synonym Full Names

ribosomal protein S20

NCBI Official Symbol

RPS20

NCBI Official Synonym Symbols

S20

UniProt Protein Name

40S ribosomal protein S20

UniProt Synonym Protein Names

Small ribosomal subunit protein uS10

Protein Family

40S ribosomal protein

UniProt Gene Name

RPS20

NCBI Protein Information

40S ribosomal protein S20

NCBI Summary for RPS20

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene U54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2009]

Research Articles on RPS20

1. We therefore found published evidence to support the association between variants in NTHL1 and RPS20 with CRC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-RPS20 antibody

Products by Pathway	Pathway Diagram
Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies	Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
Cap-dependent Translation Initiation Pathway antibodies	Cap-dependent Translation Initiation Pathway Diagram
Cytoplasmic Ribosomal Proteins Pathway antibodies	Cytoplasmic Ribosomal Proteins Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Eukaryotic Translation Elongation Pathway antibodies	Eukaryotic Translation Elongation Pathway Diagram
Eukaryotic Translation Initiation Pathway antibodies	Eukaryotic Translation Initiation Pathway Diagram
Eukaryotic Translation Termination Pathway antibodies	Eukaryotic Translation Termination Pathway Diagram
Formation Of A Pool Of Free 40S Subunits Pathway antibodies	Formation Of A Pool Of Free 40S Subunits Pathway Diagram
Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway antibodies	Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway Diagram
GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies	GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram

Diseases associated with anti-RPS20 antibody

Disease Name	Pubmed Publications
Liver Cirrhosis Antibodies	>1 publications with RPS20 and Liver Cirrhosis
Hepatitis Antibodies	>1 publications with RPS20 and Hepatitis
Liver Diseases Antibodies	>1 publications with RPS20 and Liver Diseases
Disease Models, Animal Antibodies	>1 publications with RPS20 and Disease Models, Animal
Kidney Diseases Antibodies	>1 publications with RPS20 and Kidney Diseases
Necrosis Antibodies	>1 publications with RPS20 and Necrosis

Organs/Tissues associated with anti-RPS20 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>4 publications with RPS20 and Blood
Kidney Antibodies	>2 publications with RPS20 and Kidney
Liver Antibodies	>2 publications with RPS20 and Liver
Heart Antibodies	>1 publications with RPS20 and Heart
Skin Antibodies	>1 publications with RPS20 and Skin
Bone Marrow Antibodies	>1 publications with RPS20 and Bone Marrow
Uterus Antibodies	>1 publications with RPS20 and Uterus
Muscle Antibodies	>1 publications with RPS20 and Muscle
Ovary Antibodies	>1 publications with RPS20 and Ovary
Intestine Antibodies	>1 publications with RPS20 and Intestine