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Tyrosine Hydroxylase (TH) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS175030

Description: Mouse IgG monoclonal antibody for Tyrosine Hydroxylase, tyrosine hydroxylase (TH) detection. Tested with WB, IHC-P, IHC-F in Human, rat, rabbit. No cross reactivity with other proteins. Uniprot ID: P07101Background: Tyrosine hydroxylase is… More details

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Product Overview

Online Datasheet in PDF
Product Name
Tyrosine Hydroxylase (TH), Monoclonal Antibody
Full Product Name
Anti-Tyrosine Hydroxylase antibody (monoclonal)
Product Gene Name
anti-TH antibody
Product Synonym Names
Tyrosine 3-monooxygenase; tyrosine hydroxylase; TYH; DYT5b; tyrosine 3-monooxygenase
Clonality
Monoclonal
Isotype
IgG1
Clone Number
TH-100
Host
Mouse
Species Reactivity
Human, Rat, Rabbit
Form/Format
Lyophilized
Purity/Purification
Ascites
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
44,898 Da
OMIM
gene 605407
3D Structure
ModBase 3D Structure for P07101
Immunogen
Rat tyrosine hydroxylase (TH).
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Reconstitution
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TH antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin

Immunohistochemistry (IHC) of anti-TH antibody

Figure 1. IHC analysis of Tyrosine Hydroxylase using anti- Tyrosine Hydroxylase antibody (MBS175030).
Tyrosine Hydroxylase was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Tyrosine Hydroxylase Antibody (MBS175030) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Mouse Anti-TH Monoclonal Antibody – Cat# MBS175030

Western Blot (WB) of anti-TH antibody

Figure 2. Western blot analysis of Tyrosine Hydroxylase using anti- Tyrosine Hydroxylase antibody (MBS175030).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Tyrosine Hydroxylase antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Tyrosine Hydroxylase at approximately 59KD. The expected band size for Tyrosine Hydroxylase is at 59KD.
Mouse Anti-TH Monoclonal Antibody, Western Blot (WB) – Cat# MBS175030

Other Notes

Small volumes of anti-TH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TH purchase

MBS175030 is an antibody targeting TH. Also known as Anti-Tyrosine Hydroxylase antibody (monoclonal). Reactive in Human, Rat, Rabbit. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TH. It may not necessarily be applicable to this product.

NCBI GI #
239938945
NCBI GeneID
7054
NCBI Accession #
P07101.5
UniProt Primary Accession #
P07101
UniProt Secondary Accession #
Q0PWM2; Q0PWM3; Q15585; Q15588; Q15589; Q2M3B4; B7ZL70; B7ZL73
UniProt Related Accession #
P07101
NCBI Official Full Name
Tyrosine 3-monooxygenase
NCBI Official Synonym Full Names
tyrosine hydroxylase
NCBI Official Symbol
TH
NCBI Official Synonym Symbols
TYH; DYT14; DYT5b
UniProt Protein Name
Tyrosine 3-monooxygenase
UniProt Synonym Protein Names
Tyrosine 3-hydroxylase; TH
UniProt Gene Name
TH
UniProt Synonym Gene Names
TYH; TH
UniProt Entry Name
TY3H_HUMAN
NCBI Protein Information
tyrosine 3-monooxygenase; dystonia 14; tyrosine 3-hydroxylase

NCBI Summary for TH

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for TH

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Mitochondrial; Endoplasmic reticulum; Amino Acid Metabolism - tyrosine; EC 1.14.16.2; Oxidoreductase; Vesicle

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: synaptic vesicle; internal side of plasma membrane; neuron projection; smooth endoplasmic reticulum; mitochondrion; melanosome membrane; dendrite; cytoplasm; terminal button; perikaryon; cytoplasmic vesicle; cytosol; nucleus

Molecular Function: protein domain specific binding; amino acid binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity

Biological Process: heart morphogenesis; response to peptide hormone stimulus; synaptic transmission, dopaminergic; heart development; locomotory behavior; response to lipopolysaccharide; dopamine biosynthetic process; fatty acid metabolic process; response to estradiol stimulus; regulation of heart contraction; response to salt stress; phytoalexin metabolic process; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; sensory perception of sound; visual perception; response to electrical stimulus; glycoside metabolic process; epinephrine biosynthetic process; neurotransmitter biosynthetic process; response to pyrethroid; response to corticosterone stimulus; response to nutrient levels; response to light stimulus; anatomical structure morphogenesis; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; phthalate metabolic process; mating behavior; multicellular organismal aging; isoquinoline alkaloid metabolic process; response to herbicide; social behavior; learning; response to ether; synaptic vesicle amine transport; memory; organ morphogenesis; eye photoreceptor cell development; response to ethanol; circadian sleep/wake cycle; pigmentation; response to zinc ion; response to hypoxia; cerebral cortex development; response to activity; embryonic camera-type eye morphogenesis; response to water deprivation

Disease: Segawa Syndrome, Autosomal Recessive
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Research Articles on TH

1. Tyrosine hydroxylase polymorphisms contribute to attempted suicide in schizophrenia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TH antibody

 Reference Product  PubMed Publications
 JUN antibody  >536885 publications with TH and JUN
 GAL antibody  >3297 publications with TH and GAL
 FOS antibody  >2744 publications with TH and FOS
 NGF antibody  >1827 publications with TH and NGF
 TYR antibody  >1607 publications with TH and TYR
 DDC antibody  >678 publications with TH and DDC
 FOSB antibody  >113 publications with TH and FOSB
 MAPKAPK2 antibody  >78 publications with TH and MAPKAPK2
 YWHAZ antibody  >15 publications with TH and YWHAZ
 CAMK2G antibody  >5 publications with TH and CAMK2G

Pathways associated with anti-TH antibody

 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alpha-synuclein Signaling Pathway antibodies  Alpha-synuclein Signaling Pathway Diagram
 Amine-derived Hormones Pathway antibodies  Amine-derived Hormones Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Catecholamine Biosynthesis Pathway antibodies  Catecholamine Biosynthesis Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram

Diseases associated with anti-TH antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >901707 publications with TH and Nervous System Diseases
 Heart Diseases Antibodies  >494712 publications with TH and Heart Diseases
 Brain Diseases Antibodies  >432301 publications with TH and Brain Diseases
 Pain Antibodies  >333314 publications with TH and Pain
 Kidney Diseases Antibodies  >215266 publications with TH and Kidney Diseases
 Inflammation Antibodies  >181408 publications with TH and Inflammation
 Hypertension Antibodies  >177768 publications with TH and Hypertension
 Necrosis Antibodies  >116029 publications with TH and Necrosis
 Myocardial Infarction Antibodies  >107409 publications with TH and Myocardial Infarction
 Arrhythmias, Cardiac Antibodies  >98557 publications with TH and Arrhythmias, Cardiac

Organs/Tissues associated with anti-TH antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >294038 publications with TH and Brain
 Eye Antibodies  >150372 publications with TH and Eye
 Nerve Antibodies  >131579 publications with TH and Nerve
 Mouth Antibodies  >105671 publications with TH and Mouth
 Pancreas Antibodies  >42673 publications with TH and Pancreas
 Adrenal Gland Antibodies  >19793 publications with TH and Adrenal Gland
 Placenta Antibodies  >9323 publications with TH and Placenta
 Uncharacterized Tissue Antibodies  >111 publications with TH and Uncharacterized Tissue
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