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TFPI Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750554

Description: TFPI (Tissue factor pathway inhibitor) also known as EPI, LACI, TFI and TFPT1 is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). While Xa is inhibited, the Xa-TFPI complex can subsequently also… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TFPI, Polyclonal Antibody
Full Product Name
Anti-TFPI Picoband Antibody
Product Gene Name
anti-TFPI antibody
Product Synonym Names
Tissue factor pathway inhibitor; TFPI; Extrinsic pathway inhibitor; EPI; Lipoprotein-associated coagulation inhibitor; LACI; TFPI; LACI; TFPI1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified
Concentration
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
35015 MW
OMIM
J03225 mRNA
3D Structure
ModBase 3D Structure for P10646
Immunogen
E Coli-derived human TFPI recombinant protein (Position: N253-M304). Human TFPI shares 51.1% and 53.2% amino acid (aa) sequence identity with mouse and rat TFPI, respectively.
Subcellular Localization
Isoform Alpha: Secreted.
Tissue Specificity
Mostly in endothelial cells.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-TFPI antibody

Cardiovascular; Blood; Platelets; Cardiovascular; Coagulation; Extrinsic
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TFPI antibody
Western Blot (WB)
Application Notes for anti-TFPI antibody
WB: 0.1-0.5mug/ml

Western Blot (WB) of anti-TFPI antibody

Figure 1. Western blot analysis of TFPI using anti- TFPI antibody (MBS1750554).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole Cell lysates,
Lane 2: MCF-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- TFPI antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TFPI at approximately 45KD. The expected band size for TFPI is at 35KD.
Rabbit Anti-TFPI Polyclonal Antibody – Cat# MBS1750554

Other Notes

Small volumes of anti-TFPI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TFPI purchase

MBS1750554 is an antibody targeting TFPI. Also known as Anti-TFPI Picoband Antibody. Reactive in Human
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TFPI. It may not necessarily be applicable to this product.

NCBI GI #
73760409
NCBI GeneID
7035
NCBI Accession #
NP_001027452.1
UniProt Primary Accession #
P10646
UniProt Secondary Accession #
O95103; Q53TS4
UniProt Related Accession #
P10646
GenBank Nucleotide #
NM_001032281.3
NCBI Official Full Name
tissue factor pathway inhibitor isoform b
NCBI Official Synonym Full Names
tissue factor pathway inhibitor
NCBI Official Symbol
TFPI
NCBI Official Synonym Symbols
EPI; TFI; LACI; TFPI1
UniProt Protein Name
Tissue factor pathway inhibitor
UniProt Synonym Protein Names
Extrinsic pathway inhibitor; EPI; Lipoprotein-associated coagulation inhibitor; LACI
Protein Family
Tissue factor pathway inhibitor
UniProt Gene Name
TFPI
UniProt Synonym Gene Names
LACI; TFPI1; TFPI; EPI; LACI
NCBI Protein Information
tissue factor pathway inhibitor

NCBI Summary for TFPI

This gene encodes a Kunitz-type serine protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. Inhibition of the encoded protein restores hemostasis in animal models of hemophilia. This gene encodes multiple protein isoforms that differ in their inhibitory activity, specificity and cellular localization. [provided by RefSeq, Jul 2016]
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UniProt Comments for TFPI

Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.

Research Articles on TFPI

1. miR-27a/b and miR-494 regulate TFPIalpha expression; there may be a role of these miRNAs in the estrogen-mediated downregulation of TFPIalpha

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TFPI antibody

 Reference Product  PubMed Publications
 FGF2 antibody  >11 publications with TFPI and FGF2
 F10 antibody  >2 publications with TFPI and F10
 SERPINC1 antibody  >1 publications with TFPI and SERPINC1
 SDC4 antibody  >1 publications with TFPI and SDC4

Pathways associated with anti-TFPI antibody

 Products by Pathway  Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Extrinsic Pathway antibodies  Extrinsic Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Integrated Breast Cancer Pathway antibodies  Integrated Breast Cancer Pathway Diagram
 Syndecan-4-mediated Signaling Events Pathway antibodies  Syndecan-4-mediated Signaling Events Pathway Diagram

Diseases associated with anti-TFPI antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >517 publications with TFPI and Cardiovascular Diseases
 Inflammation Antibodies  >188 publications with TFPI and Inflammation
 Neoplasms Antibodies  >183 publications with TFPI and Neoplasms
 Venous Thrombosis Antibodies  >124 publications with TFPI and Venous Thrombosis
 Necrosis Antibodies  >77 publications with TFPI and Necrosis
 Disease Models, Animal Antibodies  >73 publications with TFPI and Disease Models, Animal
 Carcinoma Antibodies  >72 publications with TFPI and Carcinoma
 Myocardial Infarction Antibodies  >64 publications with TFPI and Myocardial Infarction
 Kidney Diseases Antibodies  >57 publications with TFPI and Kidney Diseases
 Pain Antibodies  >37 publications with TFPI and Pain

Organs/Tissues associated with anti-TFPI antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1198 publications with TFPI and Blood
 Vascular Antibodies  >521 publications with TFPI and Vascular
 Embryonic Tissue Antibodies  >100 publications with TFPI and Embryonic Tissue
 Muscle Antibodies  >95 publications with TFPI and Muscle
 Lung Antibodies  >84 publications with TFPI and Lung
 Heart Antibodies  >81 publications with TFPI and Heart
 Kidney Antibodies  >79 publications with TFPI and Kidney
 Liver Antibodies  >75 publications with TFPI and Liver
 Umbilical Cord Antibodies  >46 publications with TFPI and Umbilical Cord
 Placenta Antibodies  >46 publications with TFPI and Placenta
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