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RPGR Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750662

Description: X-linked retinitis pigmentosa GTPase regulator is a GTPase-binding protein that in humans is encoded by the RPGR gene. This gene encodes a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
RPGR, Polyclonal Antibody
Full Product Name
Anti-RPGR Picoband antibody
Product Gene Name
anti-RPGR antibody
Product Synonym Names
X-linked retinitis pigmentosa GTPase regulator; RPGR; RP3; XLRP3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
127,042 Da
OMIM
U57629 mRNA
3D Structure
ModBase 3D Structure for Q92834
Immunogen
E Coli-derived human RPGR recombinant protein (Position: A13-F338).
Subcellular Localization
Cytoplasm, cytoskeleton, flagellum axoneme.
Tissue Specificity
Heart, brain, placenta, lung, liver, muscle, kidney, retina, pancreas and fetal retinal pigment epithelium. Isoform 3 is found only in the retina. Colocalizes with RPGRIP1 in the outer segment of rod photoreceptors and cone outer segments.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-RPGR antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-RPGR antibody
WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Western Blot (WB) of anti-RPGR antibody

Figure 1. Western blot analysis of RPGR using anti-RPGR antibody (MBS1750662).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPGR antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPGR at approximately 113KD. The expected band size for RPGR is at 113KD.
Rabbit Anti-RPGR Polyclonal Antibody – Cat# MBS1750662

Other Notes

Small volumes of anti-RPGR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPGR purchase

MBS1750662 is an antibody targeting RPGR. Also known as Anti-RPGR Picoband antibody. Reactive in Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RPGR. It may not necessarily be applicable to this product.

NCBI GI #
4506581
NCBI GeneID
6103
NCBI Accession #
NP_000319.1
UniProt Primary Accession #
Q92834
UniProt Secondary Accession #
O00702; O00737; Q3KN84; Q8N5T6; Q93039; Q9HD29; Q9UMR1; B1ARN3; E9PE28
UniProt Related Accession #
Q92834
GenBank Nucleotide #
NM_000328.2
NCBI Official Full Name
X-linked retinitis pigmentosa GTPase regulator isoform A
NCBI Official Synonym Full Names
retinitis pigmentosa GTPase regulator
NCBI Official Symbol
RPGR
NCBI Official Synonym Symbols
CRD; RP3; COD1; PCDX; RP15; XLRP3; orf15; CORDX1
UniProt Protein Name
X-linked retinitis pigmentosa GTPase regulator
Protein Family
X-linked retinitis pigmentosa GTPase regulator
UniProt Gene Name
RPGR
UniProt Synonym Gene Names
RP3; XLRP3
NCBI Protein Information
X-linked retinitis pigmentosa GTPase regulator

NCBI Summary for RPGR

This gene encodes a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine nucleotide exchange factors. The encoded protein is found in the Golgi body and interacts with RPGRIP1. This protein localizes to the outer segment of rod photoreceptors and is essential for their viability. Mutations in this gene have been associated with X-linked retinitis pigmentosa (XLRP). Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length natures of only some have been determined. [provided by RefSeq, Dec 2008]
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UniProt Comments for RPGR

Could be a guanine-nucleotide releasing factor. Plays a role in ciliogenesis. Probably regulates cilia formation by regulating actin stress filaments and cell contractility. Plays an important role in photoreceptor integrity. May play a critical role in spermatogenesis and in intraflagellar transport processes (). May be involved in microtubule organization and regulation of transport in primary cilia.
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Research Articles on RPGR

1. In induced pluripotent stem cells and mouse knockouts that RPGR mediates actin dynamics in photoreceptors via the actin-severing protein, gelsolin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPGR antibody

 Reference Product  PubMed Publications
 RPGRIP1 antibody  >28 publications with RPGR and RPGRIP1
 SMC3 antibody  >2 publications with RPGR and SMC3
 IFT88 antibody  >2 publications with RPGR and IFT88
 PDE6D antibody  >1 publications with RPGR and PDE6D
 NPM1 antibody  >1 publications with RPGR and NPM1
 KIF3A antibody  >1 publications with RPGR and KIF3A

Diseases associated with anti-RPGR antibody

 Disease Name  Pubmed Publications
 RETINITIS PIGMENTOSA, X-LINKED, AND SINORESPIRATORY INFECTIONS, WITH OR WITHOUT DEAFNESS Antibodies  >31519 publications with RPGR and RETINITIS PIGMENTOSA, X-LINKED, AND SINORESPIRATORY INFECTIONS, WITH OR WITHOUT DEAFNESS
 Retinitis Pigmentosa Antibodies  >195 publications with RPGR and Retinitis Pigmentosa
 Eye Diseases Antibodies  >177 publications with RPGR and Eye Diseases
 Retinal Degeneration Antibodies  >173 publications with RPGR and Retinal Degeneration
 Retinal Dystrophies Antibodies  >155 publications with RPGR and Retinal Dystrophies
 CONE-ROD DYSTROPHY, X-LINKED, 1 Antibodies  >78 publications with RPGR and CONE-ROD DYSTROPHY, X-LINKED, 1
 Atrophy Antibodies  >17 publications with RPGR and Atrophy
 Hearing Loss Antibodies  >11 publications with RPGR and Hearing Loss
 Hearing Disorders Antibodies  >9 publications with RPGR and Hearing Disorders
 Macular Degeneration Antibodies  >6 publications with RPGR and Macular Degeneration

Organs/Tissues associated with anti-RPGR antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >190 publications with RPGR and Eye
 Blood Antibodies  >16 publications with RPGR and Blood
 Kidney Antibodies  >10 publications with RPGR and Kidney
 Brain Antibodies  >4 publications with RPGR and Brain
 Liver Antibodies  >3 publications with RPGR and Liver
 Testis Antibodies  >3 publications with RPGR and Testis
 Embryonic Tissue Antibodies  >2 publications with RPGR and Embryonic Tissue
 Lymph Node Antibodies  >1 publications with RPGR and Lymph Node
 Lung Antibodies  >1 publications with RPGR and Lung
 Vascular Antibodies  >1 publications with RPGR and Vascular
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