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HSP27 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751266

Description: Rabbit IgG polyclonal antibody for Hsp27 detection. Tested with WB, IHC-P, Direct ELISA in Mouse; Rat.Background: HSPB1 (Heat shock 27kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. It is mapped to… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Hsp27/Hspb1, Polyclonal Antibody
Full Product Name
Anti-Hsp27/Hspb1 Antibody
Product Gene Name
anti-Hsp27 antibody
Product Synonym Gene Name
Hspb1
Product Synonym Names
Heat shock protein beta-1; HspB1; Growth-related 25 kDa protein; Heat shock 25 kDa protein; HSP 25; Heat shock 27 kDa protein; HSP 27; p25; Hspb1; Hsp25; Hsp27; Heat shock protein 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
21,961 Da
3D Structure
ModBase 3D Structure for P14602
Immunogen
E Coli-derived mouse Hsp27 recombinant protein (Position: M1-K209).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Hsp27 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes for anti-Hsp27 antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Western Blot (WB) of anti-Hsp27 antibody

Figure 1. Western blot analysis of Hsp27 using anti-Hsp27 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: mouse spleen tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp27 antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Hsp27 at approximately 27KD. The expected band size for Hsp27 is at 27KD.
Rabbit Anti-HSP27 Polyclonal Antibody – Cat# MBS1751266

Immunohistochemistry (IHC) of anti-Hsp27 antibody

Figure 3. IHC analysis of Hsp27 using anti-Hsp27 antibody.
Hsp27 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hsp27 antibody.
Hsp27 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hsp27 antibody.
Hsp27 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hsp27 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-HSP27 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751266

Product References and Citations for anti-Hsp27 antibody

1. Ackerley, S., James, P. A., Kalli, A., French, S., Davies, K. E., Talbot, K. A mutation in the small heat-shock protein HSPB1 leading to distal hereditary motor neuronopathy disrupts neurofilament assembly and the axonal transport of specific cellular cargoes. Hum. Molec. Genet. 15: 347-354, 2006. 2. d'Ydewalle, C., Krishnan, J., Chiheb, D. M., Van Damme, P., Irobi, J., Kozikowski, A. P., Vanden Berghe, P., Timmerman, V., Robberecht, W., Van Den Bosch, L. HDAC6 inhibitors reverse axonal loss in a mouse model of mutant HSPB1-induced Charcot-Marie-Tooth disease. Nature Med. 17: 968-974, 2011. 3. Stock, A. D., Spallone, P. A., Dennis, T. R., Netski, D., Morris, C. A., Mervis, C. B., Hobart, H. H. Heat shock protein 27 gene: chromosomal and molecular location and relationship to Williams syndrome. Am. J. Med. Genet. 120A: 320-325, 2003.

Other Notes

Small volumes of anti-Hsp27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Hsp27 purchase

MBS1751266 is an antibody targeting Hsp27. Also known as Anti-Hsp27/Hspb1 Antibody. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Hsp27. It may not necessarily be applicable to this product.

NCBI GI #
158937312
NCBI GeneID
15507
NCBI Accession #
NP_038588.2
UniProt Primary Accession #
P14602
UniProt Related Accession #
P14602
GenBank Nucleotide #
NM_013560.2
NCBI Official Full Name
heat shock protein beta-1
NCBI Official Synonym Full Names
heat shock protein 1
NCBI Official Symbol
Hspb1
NCBI Official Synonym Symbols
27kDa; Hsp25
UniProt Protein Name
Heat shock protein beta-1
UniProt Synonym Protein Names
Growth-related 25 kDa protein; Heat shock 25 kDa protein; HSP 25; Heat shock 27 kDa protein; HSP 27; p25
Protein Family
Heat shock protein
UniProt Gene Name
Hspb1
UniProt Synonym Gene Names
Hsp25; Hsp27; HspB1; HSP 25; HSP 27
NCBI Protein Information
heat shock protein beta-1

UniProt Comments for Hsp27

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization (PubMed:17661394). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins ().
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Research Articles on Hsp27

1. HSP27 functions as a negative regulator in the PDGF-BB-stimulated migration of osteoblasts.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Hsp27 antibody

 Reference Product  PubMed Publications
 Mapkapk2 antibody  >58 publications with Hsp27 and Mapkapk2
 Hsf1 antibody  >49 publications with Hsp27 and Hsf1
 Hspa1a antibody  >25 publications with Hsp27 and Hspa1a
 Hspa8 antibody  >21 publications with Hsp27 and Hspa8
 Mapk14 antibody  >7 publications with Hsp27 and Mapk14
 Akt1 antibody  >6 publications with Hsp27 and Akt1
 Hspa1b antibody  >5 publications with Hsp27 and Hspa1b
 Mapkapk5 antibody  >5 publications with Hsp27 and Mapkapk5
 Mapkapk3 antibody  >4 publications with Hsp27 and Mapkapk3
 Eif4g1 antibody  >1 publications with Hsp27 and Eif4g1

Pathways associated with anti-Hsp27 antibody

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 FAS Pathway And Stress Induction Of HSP Regulation antibodies  FAS Pathway And Stress Induction Of HSP Regulation Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 IL-3 Signaling Pathway antibodies  IL-3 Signaling Pathway Diagram
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Diseases associated with anti-Hsp27 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >109 publications with Hsp27 and Disease Models, Animal
 Inflammation Antibodies  >79 publications with Hsp27 and Inflammation
 Necrosis Antibodies  >76 publications with Hsp27 and Necrosis
 Breast Neoplasms Antibodies  >70 publications with Hsp27 and Breast Neoplasms
 Heart Diseases Antibodies  >64 publications with Hsp27 and Heart Diseases
 Liver Diseases Antibodies  >47 publications with Hsp27 and Liver Diseases
 Reperfusion Injury Antibodies  >38 publications with Hsp27 and Reperfusion Injury
 Kidney Diseases Antibodies  >37 publications with Hsp27 and Kidney Diseases
 Lung Neoplasms Antibodies  >36 publications with Hsp27 and Lung Neoplasms
 Liver Neoplasms Antibodies  >31 publications with Hsp27 and Liver Neoplasms

Organs/Tissues associated with anti-Hsp27 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >251 publications with Hsp27 and Muscle
 Brain Antibodies  >182 publications with Hsp27 and Brain
 Heart Antibodies  >126 publications with Hsp27 and Heart
 Liver Antibodies  >89 publications with Hsp27 and Liver
 Lung Antibodies  >84 publications with Hsp27 and Lung
 Kidney Antibodies  >80 publications with Hsp27 and Kidney
 Eye Antibodies  >51 publications with Hsp27 and Eye
 Skin Antibodies  >47 publications with Hsp27 and Skin
 Embryonic Tissue Antibodies  >37 publications with Hsp27 and Embryonic Tissue
 Intestine Antibodies  >31 publications with Hsp27 and Intestine
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