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Thioredoxin/TRX/Txn (TRX) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751316

Description: Rabbit IgG polyclonal antibody for Thioredoxin TRX detection. Tested with WB, IHC-P, ICC, Direct ELISA in Mouse; Rat.Background: Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. The… More details

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Product Overview

Online Datasheet in PDF
Product Name
Thioredoxin/TRX/Txn, Polyclonal Antibody
Full Product Name
Anti-Thioredoxin/TRX/Txn Antibody
Product Gene Name
anti-TRX antibody
Product Synonym Gene Name
TXN
Product Synonym Names
Thioredoxin; Trx; ATL-derived factor; ADF; Txn; Txn1; Thioredoxin 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
11,675 Da
3D Structure
ModBase 3D Structure for P10639
Immunogen
E Coli-derived mouse Thioredoxin TRX recombinant protein (Position: V2-A105).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P and ICC.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TRX antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Direct ELISA (EIA)
Application Notes for anti-TRX antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
ICC: 0.5-1 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Western Blot (WB) of anti-TRX antibody

Figure 1. Western blot analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse testis tissue lysates,
Lane 2: mouse kidney tissue lysates,
Lane 3: mouse small intestine tissue lysates,
Lane 4: mouse spleen tissue lysates,
Lane 5: mouse stomach tissue lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse smooth muscle tissue lysates,
Lane 8: mouse heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thioredoxin TRX antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Thioredoxin TRX at approximately 12KD. The expected band size for Thioredoxin TRX is at 12KD.
Rabbit Anti-TRX Polyclonal Antibody – Cat# MBS1751316

Immunohistochemistry (IHC) of anti-TRX antibody

Figure 3. IHC analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody.
Thioredoxin TRX was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Thioredoxin TRX antibody.
Thioredoxin TRX was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Thioredoxin TRX antibody.
Thioredoxin TRX was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Thioredoxin TRX antibody.
Thioredoxin TRX was detected in immunocytochemical section of LLC cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Thioredoxin TRX Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-TRX Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751316

Product References and Citations for anti-TRX antibody

1. Benhar, M., Forrester, M. T., Hess, D. T., Stamler, J. S. Regulated protein denitrosylation by cytosolic and mitochondrial thioredoxins. Science 320: 1050-1054, 2008. 2. Im, J.-Y., Lee, K.-W., Woo, J.-M., Junn, E., Mouradian, M. M. DJ-1 induces thioredoxin 1 expression through the Nrf2 pathway. Hum. Molec. Genet. 21: 3013-3024, 2012.

Other Notes

Small volumes of anti-TRX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRX purchase

MBS1751316 is an antibody targeting TRX. Also known as Anti-Thioredoxin/TRX/Txn Antibody. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TRX. It may not necessarily be applicable to this product.

NCBI GI #
6755911
NCBI GeneID
22166
NCBI Accession #
NP_035790.1
UniProt Primary Accession #
P10639
UniProt Secondary Accession #
Q52KC4; Q9D8R0
UniProt Related Accession #
P10639
GenBank Nucleotide #
NM_011660.3
NCBI Official Full Name
thioredoxin
NCBI Official Synonym Full Names
thioredoxin 1
NCBI Official Symbol
Txn1
NCBI Official Synonym Symbols
ADF; Txn; Trx1; AW550880
UniProt Protein Name
Thioredoxin
UniProt Synonym Protein Names
ATL-derived factor; ADF
Protein Family
Histone-lysine N-methyltransferase
UniProt Gene Name
Txn
UniProt Synonym Gene Names
Txn1; Trx; ADF
NCBI Protein Information
thioredoxin

UniProt Comments for TRX

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity ().
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Research Articles on TRX

1. Up-regulation thioredoxin resulted in decreased intracellular ROS generation and the underlying mechanism is related to inhibition of the Txnip/mTOR pathway mediated autophagy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TRX antibody

 Products by Pathway  Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Inflammasomes Pathway antibodies  Inflammasomes Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways antibodies  Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways Diagram
 Synthesis And Interconversion Of Nucleotide Di- And Triphosphates Pathway antibodies  Synthesis And Interconversion Of Nucleotide Di- And Triphosphates Pathway Diagram
 The NLRP3 Inflammasome Pathway antibodies  The NLRP3 Inflammasome Pathway Diagram

Diseases associated with anti-TRX antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >19 publications with TRX and Neoplasms
 Cardiovascular Diseases Antibodies  >16 publications with TRX and Cardiovascular Diseases
 Heart Diseases Antibodies  >14 publications with TRX and Heart Diseases
 Drug Toxicity Antibodies  >12 publications with TRX and Drug Toxicity
 Cardiomyopathies Antibodies  >10 publications with TRX and Cardiomyopathies
 Carcinoma Antibodies  >9 publications with TRX and Carcinoma
 Neoplasm Metastasis Antibodies  >9 publications with TRX and Neoplasm Metastasis
 Nervous System Diseases Antibodies  >6 publications with TRX and Nervous System Diseases
 Lung Diseases Antibodies  >6 publications with TRX and Lung Diseases
 Myocardial Infarction Antibodies  >6 publications with TRX and Myocardial Infarction

Organs/Tissues associated with anti-TRX antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >14 publications with TRX and Muscle
 Heart Antibodies  >14 publications with TRX and Heart
 Blood Antibodies  >12 publications with TRX and Blood
 Brain Antibodies  >9 publications with TRX and Brain
 Liver Antibodies  >8 publications with TRX and Liver
 Lung Antibodies  >6 publications with TRX and Lung
 Kidney Antibodies  >4 publications with TRX and Kidney
 Tongue Antibodies  >3 publications with TRX and Tongue
 Prostate Antibodies  >3 publications with TRX and Prostate
 Bone Antibodies  >3 publications with TRX and Bone
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