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CDK8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751354

Description: Rabbit IgG polyclonal antibody for Cdk8 detection. Tested with WB, IHC-P, Direct ELISA in Human; Mouse; Rat.Background: Cell division protein kinase 8 is an enzyme that in humans is encoded by the CDK8 gene. It is mapped to 13q12.13. This gene… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Cdk8, Polyclonal Antibody
Full Product Name
Anti-Cdk8 Antibody
Product Gene Name
anti-Cdk8 antibody
Product Synonym Names
Cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35; CDK8; Cyclin dependent kinase 8
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
53,156 Da
3D Structure
ModBase 3D Structure for P49336
Immunogen
E Coli-derived human Cdk8 recombinant protein (K355-Y464).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Cdk8 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes for anti-Cdk8 antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Product References and Citations for anti-Cdk8 antibody

1. Akoulitchev, S., Chuikov, S., Reinberg, D. TFIIH is negatively regulated by cdk8-containing mediator complexes. Nature 407: 102-106, 2000. 2. Donner, A. J., Szostek, S., Hoover, J. M., Espinosa, J. M. CDK8 is a stimulus-specific positive coregulator of p53 target genes. Molec. Cell 27: 121-133, 2007. 3. Firestein, R., Bass, A. J., Kim, S. Y., Dunn, I. F., Silver, S. J., Guney, I., Freed, E., Ligon, A. H., Vena, N., Ogino, S., Chheda, M. G., Tamayo, P., and 16 others. CDK8 is a colorectal cancer oncogene that regulates beta-catenin activity. Nature 455: 547-551, 2008.

Other Notes

Small volumes of anti-Cdk8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cdk8 purchase

MBS1751354 is an antibody targeting Cdk8. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Cdk8. It may not necessarily be applicable to this product.

NCBI GI #
4502745
NCBI GeneID
1024
NCBI Accession #
NP_001251.1
UniProt Primary Accession #
P49336
UniProt Secondary Accession #
Q5VUF3; Q6ISB5
UniProt Related Accession #
P49336
GenBank Nucleotide #
NM_001260.3
NCBI Official Full Name
cyclin-dependent kinase 8 isoform 1
NCBI Official Synonym Full Names
cyclin dependent kinase 8
NCBI Official Symbol
CDK8
NCBI Official Synonym Symbols
K35
UniProt Protein Name
Cyclin-dependent kinase 8
UniProt Synonym Protein Names
Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35
Protein Family
Cyclin-dependent kinase
UniProt Gene Name
CDK8
NCBI Protein Information
cyclin-dependent kinase 8

NCBI Summary for Cdk8

This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]
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UniProt Comments for Cdk8

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
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Research Articles on Cdk8

1. these observations suggest that Zyxin promotes colon cancer tumorigenesis in a mitotic-phosphorylation-dependent manner and through CDK8-mediated YAP activation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Cdk8 antibody

 Reference Product  PubMed Publications
 CCNC antibody  >20 publications with Cdk8 and CCNC
 MED12 antibody  >14 publications with Cdk8 and MED12
 MAML1 antibody  >5 publications with Cdk8 and MAML1
 CCNH antibody  >4 publications with Cdk8 and CCNH
 MED21 antibody  >2 publications with Cdk8 and MED21
 MED8 antibody  >1 publications with Cdk8 and MED8
 MED26 antibody  >1 publications with Cdk8 and MED26

Pathways associated with anti-Cdk8 antibody

 Products by Pathway  Pathway Diagram
 Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 FBXW7 Mutants And NOTCH1 In Cancer Pathway antibodies  FBXW7 Mutants And NOTCH1 In Cancer Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram

Diseases associated with anti-Cdk8 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >22 publications with Cdk8 and Neoplasms
 Death Antibodies  >5 publications with Cdk8 and Death
 Leukemia Antibodies  >4 publications with Cdk8 and Leukemia
 Neoplasm Metastasis Antibodies  >3 publications with Cdk8 and Neoplasm Metastasis
 Liver Diseases Antibodies  >3 publications with Cdk8 and Liver Diseases
 Nervous System Diseases Antibodies  >3 publications with Cdk8 and Nervous System Diseases
 Cell Transformation, Neoplastic Antibodies  >3 publications with Cdk8 and Cell Transformation, Neoplastic
 Necrosis Antibodies  >2 publications with Cdk8 and Necrosis
 Neoplasms, Experimental Antibodies  >2 publications with Cdk8 and Neoplasms, Experimental
 Colonic Neoplasms Antibodies  >1 publications with Cdk8 and Colonic Neoplasms

Organs/Tissues associated with anti-Cdk8 antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >4 publications with Cdk8 and Eye
 Muscle Antibodies  >4 publications with Cdk8 and Muscle
 Heart Antibodies  >4 publications with Cdk8 and Heart
 Bone Marrow Antibodies  >3 publications with Cdk8 and Bone Marrow
 Liver Antibodies  >3 publications with Cdk8 and Liver
 Brain Antibodies  >3 publications with Cdk8 and Brain
 Prostate Antibodies  >2 publications with Cdk8 and Prostate
 Testis Antibodies  >2 publications with Cdk8 and Testis
 Lymph Antibodies  >2 publications with Cdk8 and Lymph
 Kidney Antibodies  >1 publications with Cdk8 and Kidney
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