Pegylated Human Active Protein – Cat# MBS400132

Prolactin Pegylated Human Active Protein

Name: Active Protein; Prolactin; Pegylated Human; MyBioSource
Brand: MyBioSource
SKU: MBS400132
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS400132

Recombinant human prolactin, consists of one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids was mono-pegylated and purified by proprietary chromatographic techniques as described by Oclon et al. PEDS (in press). Its molecular… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Prolactin, Active Protein

Full Product Name

Pegylated human prolactin

Sequence

The amino terminal sequence could not be determined as the N- terminal amino acid was covalently bound to polyethyle ne glycol.

Host

E Coli

Form/Format

The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO3.

Purity/Purification

Greater than 99.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.

Preparation and Storage

Lyophilized pegylated hPRL although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 pegylated hPRL (protein part) mg/ml and up to 4 mg/ml and filter sterilization pegylated hPRL can be stored at 4C for several weeks.

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Physical Appearance

White lyophilized (freeze-dried) powder.

Solubility

It is recommended to reconstitute the lyophilized pegylated hPRL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

Dimers and Aggregates

The purified mono-pegylated hPRL consists of > 95% monomers as determined by gel-filtration chromatography.

Biological Activity

pegylated hPRL is biologically active as evidenced by inducing proliferation of Nb2 cells or Baf/3 cells stably transfected with hPRL receptors, though its activity is lesser than hPRL. However it is anticipated that its activity in vivo will be higher than hPRL due to prolonged persistence in circulation.

Endotoxin

Less than 0.1 ng/ug (IEU/ug) of pegylated hPRL

Protein Content

Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.92 as the extinction coefficient for a 0.1% (1mg of protein part/ml) solution at pH 8.0. This value is calculated by the DNAman computer analysis program of protein sequences.

Other Notes

Small volumes of Prolactin active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Prolactin purchase

MBS400132 is an active protein targeting Prolactin. Also known as Pegylated human prolactin. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for Prolactin. It may not necessarily be applicable to this product.

Protein Family

Prolactin

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.