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NOVA1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751499

Description: Rabbit IgG polyclonal antibody for Nova1 detection. Tested with WB, IHC-P, IHC-F, ICC/IF, Direct ELISA in Human; Mouse; Rat.Background: RNA-binding protein Nova-1 is a protein that in humans is encoded by the NOVA1 gene. It is mapped to 14q12.… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Nova1, Polyclonal Antibody
Full Product Name
Anti-Nova1 Antibody
Product Gene Name
anti-Nova1 antibody
Product Synonym Names
RNA-binding protein Nova-1; Neuro-oncological ventral antigen 1; Onconeural ventral antigen 1; Paraneoplastic Ri antigen; Ventral neuron-specific protein 1; NOVA1; NOVA alternative splicing regulator 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20°C. After reconstitution, at 4°C. It can also be aliquotted and stored frozen at -20°C for a longer time.
Avoid repeated freezing and thawing.
3D Structure
ModBase 3D Structure for P51513
Immunogen
E Coli-derived human Nova1 recombinant protein (Position: T416-V509).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P, IHC-F and ICC.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Nova1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Frozen/Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Direct ELISA (EIA)
Application Notes for anti-Nova1 antibody
Western blot: 0.1-0.5mug/ml
Immunohistochemistry(Paraffin-embedded Section): 0.5-1mug/ml; Antigen Retrieval: By Heat
Immunohistochemistry(Frozen Section): 0.5-1mug/ml
Immunocytochemistry/Immunofluorescence: 2mug/ml
Direct ELISA: 0.1-0.5mug/ml

Tested Species: In-house tested species with positive test results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required is required for the staining of formalin/paraffin sections.

Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-Nova1 antibody

Figure 1. Western blot analysis of Nova1 using anti-Nova1 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysate,
Lane 2: human Caco-2 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nova1 antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Nova1 at approximately 60KD. The expected band size for Nova1 is at 52KD.
Rabbit Anti-NOVA1 Polyclonal Antibody – Cat# MBS1751499

Immunohistochemistry (IHC) of anti-Nova1 antibody

Figure 3. IHC analysis of Nova1 using anti-Nova1 antibody.
Nova1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Nova1 antibody.
Nova1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Nova1 antibody.
Nova1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Nova1 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-NOVA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751499

Product References and Citations for anti-Nova1 antibody

1. Jensen, K. B., Dredge, B. K., Stefani, G., Zhong, R., Buckanovich, R. J., Okano, H. J., Yang, Y. Y. L., Darnell, R. B. Nova-1 regulates neuron-specific alternative splicing and is essential for neuronal viability. Neuron 25: 359-371, 2000. 2. Licatalosi, D. D., Mele, A., Fak, J. J., Ule, J., Kayikci, M., Chi, S. W., Clark, T. A., Schweitzer, A. C., Blume, J. E., Wang, X., Darnell, J. C., Darnell, R. B.HITS-CLIP yields genome-wide insights into brain alternative RNA processing. Nature 456: 464-469, 2008. 3. Ule, J., Stefani, G., Mele, A., Ruggiu, M., Wang, X., Taneri, B., Gaasterland, T., Blencowe, B. J., Darnell, R. B. An RNA map predicting Nova-dependent splicing regulation. Nature 444: 580-586, 2006.

Other Notes

Small volumes of anti-Nova1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Nova1 purchase

MBS1751499 is an antibody targeting Nova1. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Nova1. It may not necessarily be applicable to this product.

NCBI GI #
56549653
NCBI GeneID
4857
NCBI Accession #
NP_002506.2
UniProt Primary Accession #
P51513
UniProt Secondary Accession #
Q6B004; A8K0S4; A8K4Q7; D3DS81; D3DS82
UniProt Related Accession #
P51513
GenBank Nucleotide #
NM_002515.3
NCBI Official Full Name
RNA-binding protein Nova-1 isoform 1
NCBI Official Synonym Full Names
NOVA alternative splicing regulator 1
NCBI Official Symbol
NOVA1
NCBI Official Synonym Symbols
Nova-1
UniProt Protein Name
RNA-binding protein Nova-1
UniProt Synonym Protein Names
Neuro-oncological ventral antigen 1; Onconeural ventral antigen 1; Paraneoplastic Ri antigen; Ventral neuron-specific protein 1
Protein Family
RNA-binding protein
UniProt Gene Name
NOVA1
NCBI Protein Information
RNA-binding protein Nova-1

NCBI Summary for Nova1

This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for Nova1

May regulate RNA splicing or metabolism in a specific subset of developing neurons.

Research Articles on Nova1

1. It regulates the alternative splicing of estrogen receptor beta in the aging brain.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Nova1 antibody

 Reference Product  PubMed Publications
 FOXG1 antibody  >1 publications with Nova1 and FOXG1
 REST antibody  >1 publications with Nova1 and REST

Diseases associated with anti-Nova1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >7 publications with Nova1 and Nervous System Diseases
 Brain Diseases Antibodies  >4 publications with Nova1 and Brain Diseases
 Cardiovascular Diseases Antibodies  >3 publications with Nova1 and Cardiovascular Diseases
 Carcinoma, Hepatocellular Antibodies  >2 publications with Nova1 and Carcinoma, Hepatocellular
 Inflammation Antibodies  >2 publications with Nova1 and Inflammation
 Hypertension Antibodies  >1 publications with Nova1 and Hypertension
 Seizures Antibodies  >1 publications with Nova1 and Seizures
 Nerve Degeneration Antibodies  >1 publications with Nova1 and Nerve Degeneration

Organs/Tissues associated with anti-Nova1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >13 publications with Nova1 and Brain
 Nerve Antibodies  >12 publications with Nova1 and Nerve
 Embryonic Tissue Antibodies  >2 publications with Nova1 and Embryonic Tissue
 Adipose Tissue Antibodies  >1 publications with Nova1 and Adipose Tissue
 Connective Tissue Antibodies  >1 publications with Nova1 and Connective Tissue
 Blood Antibodies  >1 publications with Nova1 and Blood
 Pancreas Antibodies  >1 publications with Nova1 and Pancreas
 Prostate Antibodies  >1 publications with Nova1 and Prostate
 Muscle Antibodies  >1 publications with Nova1 and Muscle
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