Rabbit Anti-F2 Monoclonal Antibody – Cat# MBS1751909

Figure 1. Western blot analysis of F2 using anti-F2 antibody (M00044).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-F2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for F2.

Prothrombin (F2) Antibody – Rabbit Monoclonal

Name: F2 Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1751909
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751909

Rabbit IgG Monoclonal antibody for P00734 detection. Tested positive for WB IP in Human More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Prothrombin (F2), Monoclonal Antibody

Full Product Name

Anti-Prothrombin Monoclonal Antibody

Product Gene Name

anti-F2 antibody

Clonality

Monoclonal

Isotype

IgG

Clone Number

ACBD-6

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/mL BSA.

Purity/Purification

Affinity-Chromatography

Concentration

1.25mg/mL (lot specific)

Specificity

No cross reactivity with other proteins.

Preparation and Storage

Store at -20°C. For short term storage and frequent use, store at 4°C. Avoid repeated freeze-thaw cycles.

3D Structure

ModBase 3D Structure for P00734

Immunogen

A synthesized peptide derived from human Prothrombin.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-F2 antibody

Immunoprecipitation (IP), Western Blot (WB)

Application Notes for anti-F2 antibody

Dilute the sample so that the expected range of concentrations fall within the detection range of this kit.
If the expected range of concentration is unknown, a pilot test should be conducted to decide the optimal dilution ratio for your samples. Some PubMed article(s) citing the expression level of this target are as follows:

QC testing used:
WB 1:500~1:2000
IP 1:50

Other Notes

Small volumes of anti-F2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for F2 purchase

MBS1751909 is an antibody targeting F2. Also known as Anti-Prothrombin Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for F2. It may not necessarily be applicable to this product.

NCBI GI #

4503635

NCBI GeneID

2147

NCBI Accession #

NP_000497.1

UniProt Primary Accession #

P00734

UniProt Secondary Accession #

Q4QZ40; Q53H04; Q53H06; Q69EZ7; Q7Z7P3; Q9UCA1; B2R7F7; B4E1A7

UniProt Related Accession #

P00734

GenBank Nucleotide #

NM_000506.4

NCBI Official Full Name

prothrombin isoform 1 preproprotein

NCBI Official Synonym Full Names

coagulation factor II, thrombin

NCBI Official Symbol

NCBI Official Synonym Symbols

PT; THPH1; RPRGL2

UniProt Protein Name

Prothrombin

UniProt Synonym Protein Names

Coagulation factor II

Protein Family

I-Kappa-B like protein

UniProt Gene Name

NCBI Protein Information

prothrombin

NCBI Summary for F2

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

UniProt Comments for F2

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.

Research Articles on F2

1. This study proved that Berberine (BBR) is a direct thrombin inhibitor that has activity in inhibiting thrombin-induced platelet aggregation. BBR may be a potential candidate for the development of safe and effective thrombin-inhibiting drugs

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-F2 antibody

Reference Product	PubMed Publications
PROC antibody	>227 publications with F2 and PROC
SERPINE1 antibody	>23 publications with F2 and SERPINE1
F11 antibody	>17 publications with F2 and F11
FGA antibody	>8 publications with F2 and FGA
SPP1 antibody	>8 publications with F2 and SPP1
IGFBP3 antibody	>5 publications with F2 and IGFBP3
GP1BA antibody	>5 publications with F2 and GP1BA
F2R antibody	>5 publications with F2 and F2R
F13A1 antibody	>4 publications with F2 and F13A1
IGFBP5 antibody	>3 publications with F2 and IGFBP5

Pathways associated with anti-F2 antibody

Products by Pathway	Pathway Diagram
Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies	Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
Blood Clotting Cascade Pathway antibodies	Blood Clotting Cascade Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Common Pathway antibodies	Common Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies	FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies	Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram

Diseases associated with anti-F2 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>1029 publications with F2 and Nervous System Diseases
Liver Diseases Antibodies	>695 publications with F2 and Liver Diseases
Neoplasms Antibodies	>676 publications with F2 and Neoplasms
Hypertension Antibodies	>657 publications with F2 and Hypertension
Drug Toxicity Antibodies	>611 publications with F2 and Drug Toxicity
Inflammation Antibodies	>516 publications with F2 and Inflammation
Liver Cirrhosis Antibodies	>461 publications with F2 and Liver Cirrhosis
Brain Diseases Antibodies	>452 publications with F2 and Brain Diseases
Heart Diseases Antibodies	>297 publications with F2 and Heart Diseases
Kidney Diseases Antibodies	>255 publications with F2 and Kidney Diseases

Organs/Tissues associated with anti-F2 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>1271 publications with F2 and Liver
Uterus Antibodies	>952 publications with F2 and Uterus
Kidney Antibodies	>718 publications with F2 and Kidney
Heart Antibodies	>676 publications with F2 and Heart
Embryonic Tissue Antibodies	>365 publications with F2 and Embryonic Tissue
Connective Tissue Antibodies	>251 publications with F2 and Connective Tissue
Spleen Antibodies	>229 publications with F2 and Spleen
Bone Marrow Antibodies	>104 publications with F2 and Bone Marrow
Mammary Gland Antibodies	>32 publications with F2 and Mammary Gland
Uncharacterized Tissue Antibodies	>2 publications with F2 and Uncharacterized Tissue