Rabbit Anti-ACTN4 Monoclonal Antibody – Cat# MBS1752157

Figure 1. Western blot analysis of ACTN4 using anti-ACTN4 antibody (M01975).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTN4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACTN4.

Alpha Actinin 4 (ACTN4) Antibody – Rabbit Monoclonal

Name: ACTN4 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1752157
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752157

Rabbit IgG Monoclonal antibody for O43707 detection. Tested positive for WB IHC IF/IF IP FC in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

alpha Actinin 4 (ACTN4), Monoclonal Antibody

Full Product Name

Anti-alpha Actinin 4 Monoclonal Antibody

Product Gene Name

anti-ACTN4 antibody

Clonality

Monoclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Purity/Purification

Affinity-Chromatography

Specificity

No cross reactivity with other proteins.

Preparation and Storage

Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.

Molecular Weight

79,913 Da

3D Structure

ModBase 3D Structure for O43707

Immunogen

A synthesized peptide derived from human alpha Actinin 4 F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-ACTN4 antibody

Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Application Notes for anti-ACTN4 antibody

WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50

Other Notes

Small volumes of anti-ACTN4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTN4 purchase

MBS1752157 is an antibody targeting ACTN4. Also known as Anti-alpha Actinin 4 Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTN4. It may not necessarily be applicable to this product.

NCBI GI #

12025678

NCBI GeneID

NCBI Accession #

NP_004915.2

UniProt Primary Accession #

O43707

UniProt Secondary Accession #

O76048; A4K467; D6PXK4

UniProt Related Accession #

O43707

GenBank Nucleotide #

NM_004924.6

NCBI Official Full Name

alpha-actinin-4 isoform 1

NCBI Official Synonym Full Names

actinin alpha 4

NCBI Official Symbol

ACTN4

NCBI Official Synonym Symbols

FSGS; FSGS1; ACTININ-4

UniProt Protein Name

Alpha-actinin-4

UniProt Synonym Protein Names

Non-muscle alpha-actinin 4

Protein Family

Alpha-actinin

UniProt Gene Name

ACTN4

NCBI Protein Information

alpha-actinin-4

NCBI Summary for ACTN4

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008]

UniProt Comments for ACTN4

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778).

Research Articles on ACTN4

1. significantly upregulated in the cervical mucus of women with preterm labor with microbial invasion of the amniotic cavity

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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