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Reelin (RELN) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752174

Rabbit IgG Monoclonal antibody for P78509 detection. Tested positive for WB in Human Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Reelin (RELN), Monoclonal Antibody
Full Product Name
Anti-Reelin Monoclonal Antibody
Product Gene Name
anti-RELN antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
388,201 Da
3D Structure
ModBase 3D Structure for P78509
Immunogen
A synthesized peptide derived from human Reelin Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-RELN antibody
Western Blot (WB)
Application Notes for anti-RELN antibody
WB: 1:500-1:2000

Western Blot (WB) of anti-RELN antibody

Figure 1. Western blot analysis of RELN using anti-RELN antibody (M02113).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RELN antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RELN.
Rabbit Anti-RELN Monoclonal Antibody – Cat# MBS1752174

Other Notes

Small volumes of anti-RELN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RELN purchase

MBS1752174 is an antibody targeting RELN. Also known as Anti-Reelin Monoclonal Antibody. Reactive in Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RELN. It may not necessarily be applicable to this product.

NCBI GI #
27436938
NCBI GeneID
5649
NCBI Accession #
NP_005036.2
UniProt Primary Accession #
P78509
UniProt Secondary Accession #
Q86UJ0; Q86UJ8; Q8NDV0; Q9UDQ2; A4D0P9; A4D0Q0
UniProt Related Accession #
P78509
GenBank Nucleotide #
NM_005045.4
NCBI Official Full Name
reelin isoform a
NCBI Official Synonym Full Names
reelin
NCBI Official Symbol
RELN
NCBI Official Synonym Symbols
RL; ETL7; LIS2; PRO1598
UniProt Protein Name
Reelin
Protein Family
Reelin
UniProt Gene Name
RELN
NCBI Protein Information
reelin

NCBI Summary for RELN

This gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorder, major depression and in migration defects associated with temporal lobe epilepsy. Mutations of this gene are associated with autosomal recessive lissencephaly with cerebellar hypoplasia. Two transcript variants encoding distinct isoforms have been identified for this gene. Other transcript variants have been described but their full length nature has not been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for RELN

Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation ().
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Research Articles on RELN

1. Study found a positive link between RELN SNP rs2229864 and ASD risk, but not a significant association between RELN SNPs rs736707 and autism risk. A significant rs736707-rs2229864 haplotype was found. Individuals with a CC haplotype were more likely to have autistic spectrum disorders, whereas individuals with a CT haplotype had more possibility to be typical development controls.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RELN antibody

 Reference Product  PubMed Publications
 DAB1 antibody  >35 publications with RELN and DAB1
 VLDLR antibody  >25 publications with RELN and VLDLR
 GAD1 antibody  >12 publications with RELN and GAD1
 PAFAH1B1 antibody  >9 publications with RELN and PAFAH1B1
 LRP8 antibody  >7 publications with RELN and LRP8
 GRIN2B antibody  >3 publications with RELN and GRIN2B
 GRIN2A antibody  >2 publications with RELN and GRIN2A
 FYN antibody  >2 publications with RELN and FYN
 ITGA3 antibody  >1 publications with RELN and ITGA3
 AKT1 antibody  >1 publications with RELN and AKT1

Pathways associated with anti-RELN antibody

 Products by Pathway  Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Lipoprotein Metabolism Pathway antibodies  Lipoprotein Metabolism Pathway Diagram
 Lissencephaly Gene (LIS1) In Neuronal Migration And Development Pathway antibodies  Lissencephaly Gene (LIS1) In Neuronal Migration And Development Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram

Diseases associated with anti-RELN antibody

 Disease Name  Pubmed Publications
 Schizophrenia Antibodies  >73 publications with RELN and Schizophrenia
 Nervous System Diseases Antibodies  >56 publications with RELN and Nervous System Diseases
 Autistic Disorder Antibodies  >35 publications with RELN and Autistic Disorder
 Bipolar Disorder Antibodies  >25 publications with RELN and Bipolar Disorder
 Disease Models, Animal Antibodies  >21 publications with RELN and Disease Models, Animal
 Autism Spectrum Disorder Antibodies  >18 publications with RELN and Autism Spectrum Disorder
 Movement Disorders Antibodies  >16 publications with RELN and Movement Disorders
 Alzheimer Disease Antibodies  >10 publications with RELN and Alzheimer Disease
 Cognition Disorders Antibodies  >9 publications with RELN and Cognition Disorders
 Seizures Antibodies  >7 publications with RELN and Seizures

Organs/Tissues associated with anti-RELN antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >182 publications with RELN and Brain
 Embryonic Tissue Antibodies  >22 publications with RELN and Embryonic Tissue
 Bone Antibodies  >12 publications with RELN and Bone
 Liver Antibodies  >5 publications with RELN and Liver
 Ear Antibodies  >4 publications with RELN and Ear
 Ganglia Antibodies  >3 publications with RELN and Ganglia
 Muscle Antibodies  >3 publications with RELN and Muscle
 Eye Antibodies  >3 publications with RELN and Eye
 Connective Tissue Antibodies  >2 publications with RELN and Connective Tissue
 Lung Antibodies  >2 publications with RELN and Lung
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