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MCM3 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752218

Rabbit IgG Monoclonal antibody for P25205 detection. Tested positive for WB IHC IF/IF FC in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MCM3, Monoclonal Antibody
Full Product Name
Anti-MCM3 Monoclonal Antibody
Product Gene Name
anti-MCM3 antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
95,908 Da
3D Structure
ModBase 3D Structure for P25205
Immunogen
A synthesized peptide derived from human MCM3 Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-MCM3 antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-MCM3 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50

Western Blot (WB) of anti-MCM3 antibody

Figure 1. Western blot analysis of MCM3 using anti-MCM3 antibody (M02580).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM3 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MCM3.
Rabbit Anti-MCM3 Monoclonal Antibody – Cat# MBS1752218

Other Notes

Small volumes of anti-MCM3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MCM3 purchase

MBS1752218 is an antibody targeting MCM3. Also known as Anti-MCM3 Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MCM3. It may not necessarily be applicable to this product.

NCBI GI #
394582099
NCBI GeneID
4172
NCBI Accession #
NP_001257401.1
UniProt Primary Accession #
P25205
UniProt Secondary Accession #
Q92660; Q9BTR3; Q9NUE7; B4DWW4
UniProt Related Accession #
P25205
GenBank Nucleotide #
NM_001270472.1
NCBI Official Full Name
DNA replication licensing factor MCM3 isoform 2
NCBI Official Synonym Full Names
minichromosome maintenance complex component 3
NCBI Official Symbol
MCM3
NCBI Official Synonym Symbols
HCC5; P1.h; RLFB; P1-MCM3
UniProt Protein Name
DNA replication licensing factor MCM3
UniProt Synonym Protein Names
DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3; RLF subunit beta; p102
Protein Family
DNA replication licensing factor
UniProt Gene Name
MCM3
NCBI Protein Information
DNA replication licensing factor MCM3

NCBI Summary for MCM3

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018]
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UniProt Comments for MCM3

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
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Research Articles on MCM3

1. this study shows that MCM3 is a novel proliferation marker in oral squamous cell carcinoma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MCM3 antibody

 Reference Product  PubMed Publications
 MCM2 antibody  >84 publications with MCM3 and MCM2
 MCM7 antibody  >35 publications with MCM3 and MCM7
 MCM5 antibody  >35 publications with MCM3 and MCM5
 MCM4 antibody  >32 publications with MCM3 and MCM4
 MCM6 antibody  >30 publications with MCM3 and MCM6
 CDC6 antibody  >22 publications with MCM3 and CDC6
 ORC2 antibody  >17 publications with MCM3 and ORC2
 MCM3AP antibody  >14 publications with MCM3 and MCM3AP
 CDC45 antibody  >10 publications with MCM3 and CDC45
 CDK2 antibody  >8 publications with MCM3 and CDK2

Pathways associated with anti-MCM3 antibody

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Assembly Of The Pre-replicative Complex Pathway antibodies  Assembly Of The Pre-replicative Complex Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram

Diseases associated with anti-MCM3 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >25 publications with MCM3 and Neoplasms
 Carcinoma Antibodies  >10 publications with MCM3 and Carcinoma
 Skin Diseases Antibodies  >6 publications with MCM3 and Skin Diseases
 Melanoma Antibodies  >5 publications with MCM3 and Melanoma
 Central Nervous System Diseases Antibodies  >5 publications with MCM3 and Central Nervous System Diseases
 Skin Neoplasms Antibodies  >5 publications with MCM3 and Skin Neoplasms
 Breast Neoplasms Antibodies  >4 publications with MCM3 and Breast Neoplasms
 Kidney Diseases Antibodies  >3 publications with MCM3 and Kidney Diseases
 Precancerous Conditions Antibodies  >3 publications with MCM3 and Precancerous Conditions
 Death Antibodies  >3 publications with MCM3 and Death

Organs/Tissues associated with anti-MCM3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >12 publications with MCM3 and Blood
 Skin Antibodies  >7 publications with MCM3 and Skin
 Lymph Node Antibodies  >7 publications with MCM3 and Lymph Node
 Kidney Antibodies  >6 publications with MCM3 and Kidney
 Brain Antibodies  >6 publications with MCM3 and Brain
 Lung Antibodies  >4 publications with MCM3 and Lung
 Intestine Antibodies  >3 publications with MCM3 and Intestine
 Lymph Antibodies  >3 publications with MCM3 and Lymph
 Liver Antibodies  >3 publications with MCM3 and Liver
 Uterus Antibodies  >3 publications with MCM3 and Uterus
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