Rabbit Anti-MTA2 Monoclonal Antibody – Cat# MBS1752251

Figure 1. Western blot analysis of MTA2 using anti-MTA2 antibody (M03073).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTA2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTA2.

MTA2 Antibody – Rabbit Monoclonal

Name: MTA2 Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1752251
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752251

Rabbit IgG Monoclonal antibody for O94776 detection. Tested positive for WB IHC IF/IF FC in Human More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MTA2, Monoclonal Antibody

Full Product Name

Anti-MTA2 Monoclonal Antibody

Product Gene Name

anti-MTA2 antibody

Clonality

Monoclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Purity/Purification

Affinity-Chromatography

Specificity

No cross reactivity with other proteins.

Preparation and Storage

Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.

Molecular Weight

55,037 Da

3D Structure

ModBase 3D Structure for O94776

Immunogen

A synthesized peptide derived from human MTA2 The p53 tumor suppressor gene integrates numerous signals that control cell life and death. There are several proteins that are involved in the p53 pathway, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and MTA2. The transcriptional activity of p53 is modulated by protein stability and acetylation.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-MTA2 antibody

Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Application Notes for anti-MTA2 antibody

WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:100

Other Notes

Small volumes of anti-MTA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MTA2 purchase

MBS1752251 is an antibody targeting MTA2. Also known as Anti-MTA2 Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MTA2. It may not necessarily be applicable to this product.

NCBI GI #

1060099369

NCBI GeneID

9219

NCBI Accession #

NP_001317221.1

UniProt Primary Accession #

O94776

UniProt Secondary Accession #

Q68DB1; Q9UQB5

UniProt Related Accession #

O94776

GenBank Nucleotide #

NM_001330292.1

NCBI Official Full Name

metastasis-associated protein MTA2 isoform 2

NCBI Official Synonym Full Names

metastasis associated 1 family member 2

NCBI Official Symbol

MTA2

NCBI Official Synonym Symbols

PID; MTA1L1

UniProt Protein Name

Metastasis-associated protein MTA2

UniProt Synonym Protein Names

Metastasis-associated 1-like 1; MTA1-L1 protein; p53 target protein in deacetylase complex

Protein Family

Metastasis-associated protein

UniProt Gene Name

MTA2

UniProt Synonym Gene Names

MTA1L1; PID; MTA1-L1 protein

NCBI Protein Information

metastasis-associated protein MTA2

NCBI Summary for MTA2

This gene encodes a protein that has been identified as a component of NuRD, a nucleosome remodeling deacetylase complex identified in the nucleus of human cells. It shows a very broad expression pattern and is strongly expressed in many tissues. It may represent one member of a small gene family that encode different but related proteins involved either directly or indirectly in transcriptional regulation. Their indirect effects on transcriptional regulation may include chromatin remodeling. It is closely related to another member of this family, a protein that has been correlated with the metastatic potential of certain carcinomas. These two proteins are so closely related that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. One of the proteins known to be a target protein for this gene product is p53. Deacetylation of p53 is correlated with a loss of growth inhibition in transformed cells supporting a connection between these gene family members and metastasis. [provided by RefSeq, May 2011]

UniProt Comments for MTA2

May be involved in the regulation of gene expression as repressor and activator. The repression might be related to covalent modification of histone proteins.

Research Articles on MTA2

1. This study is the first to demonstrate that SNHG5 is a critical and powerful regulator that is involved in GC progression through trapping MTA2 in the cytosol.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MTA2 antibody

Reference Product	PubMed Publications
RBBP7 antibody	>5 publications with MTA2 and RBBP7

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