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KMT5A Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752357

Rabbit IgG Monoclonal antibody for Q9NQR1 detection. Tested positive for WB FC in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KMT5A/SETD8/Pr-SET7, Monoclonal Antibody
Full Product Name
Anti-KMT5A/SETD8/Pr-SET7 Monoclonal Antibody
Product Gene Name
anti-KMT5A antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
39,223 Da
3D Structure
ModBase 3D Structure for Q9NQR1
Immunogen
A synthesized peptide derived from human KMT5A/SETD8/Pr-SET7 Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-KMT5A antibody
Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-KMT5A antibody
WB: 1:1000-1:5000
FC/FACS: 1:100

Western Blot (WB) of anti-KMT5A antibody

Figure 1. Western blot analysis of KMT5A using anti-KMT5A antibody (M05349).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KMT5A antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KMT5A.
Rabbit Anti-KMT5A Monoclonal Antibody – Cat# MBS1752357

Other Notes

Small volumes of anti-KMT5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KMT5A purchase

MBS1752357 is an antibody targeting KMT5A. Also known as Anti-KMT5A/SETD8/Pr-SET7 Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KMT5A. It may not necessarily be applicable to this product.

NCBI GI #
1027250576
NCBI GeneID
387893
NCBI Accession #
NP_001311433.1
UniProt Primary Accession #
Q9NQR1
UniProt Secondary Accession #
Q86W83; Q8TD09; A8K9D0
UniProt Related Accession #
Q9NQR1
GenBank Nucleotide #
NM_001324504.1
NCBI Official Full Name
N-lysine methyltransferase KMT5A isoform 2
NCBI Official Synonym Full Names
lysine methyltransferase 5A
NCBI Official Symbol
KMT5A
NCBI Official Synonym Symbols
SET8; SET07; SETD8; PR-Set7; PR/SET07
UniProt Protein Name
N-lysine methyltransferase KMT5A
UniProt Synonym Protein Names
H4-K20-HMTase KMT5A; Histone-lysine N-methyltransferase KMT5A (EC:2.1.1.43); Lysine N-methyltransferase 5A; Lysine-specific methylase 5A
Protein Family
N-lysine methyltransferase
UniProt Gene Name
KMT5A
UniProt Synonym Gene Names
PRSET7; SET07; SET8; SETD8; PR-Set7; PR/SET07
NCBI Protein Information
N-lysine methyltransferase KMT5A

NCBI Summary for KMT5A

The protein encoded by this gene is a protein-lysine N-methyltransferase that can monomethylate Lys-20 of histone H4 to effect transcriptional repression of some genes. The encoded protein is required for cell proliferation and plays a role in chromatin condensation. [provided by RefSeq, May 2016]

UniProt Comments for KMT5A

Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration.
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Research Articles on KMT5A

1. Results suggest that SET domain-containing protein 8 (SET8) may be a key mediator in hyperglycemic memory.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KMT5A antibody

 Reference Product  PubMed Publications
 SETD8 antibody  >19 publications with KMT5A and SETD8

Pathways associated with anti-KMT5A antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 Condensation Of Prophase Chromosomes Pathway antibodies  Condensation Of Prophase Chromosomes Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Histone Modifications Pathway antibodies  Histone Modifications Pathway Diagram
 Lysine Degradation Pathway antibodies  Lysine Degradation Pathway Diagram
 Lysine Degradation Pathway antibodies  Lysine Degradation Pathway Diagram

Diseases associated with anti-KMT5A antibody

 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >1 publications with KMT5A and Fatty Liver
 Cell Transformation, Neoplastic Antibodies  >1 publications with KMT5A and Cell Transformation, Neoplastic
 Chromosome Aberrations Antibodies  >1 publications with KMT5A and Chromosome Aberrations
 Precancerous Conditions Antibodies  >1 publications with KMT5A and Precancerous Conditions

Organs/Tissues associated with anti-KMT5A antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >2 publications with KMT5A and Liver
 Lymph Antibodies  >1 publications with KMT5A and Lymph
 Blood Antibodies  >1 publications with KMT5A and Blood
 Thyroid Antibodies  >1 publications with KMT5A and Thyroid
 Embryonic Tissue Antibodies  >1 publications with KMT5A and Embryonic Tissue
 Brain Antibodies  >1 publications with KMT5A and Brain
 Muscle Antibodies  >1 publications with KMT5A and Muscle
 Skin Antibodies  >1 publications with KMT5A and Skin
 Kidney Antibodies  >1 publications with KMT5A and Kidney
 Lymph Node Antibodies  >1 publications with KMT5A and Lymph Node
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