Rabbit Anti-ABCB5 Polyclonal Antibody – Cat# MBS1752501

ABCB5 Antibody – Rabbit Polyclonal

Name: ABCB5 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1752501
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752501

Description: Rabbit IgG polyclonal antibody for ABCB5 detection. Tested with WB, IHC-P, IHC-F, ICC, FCM, Direct ELISA in Human.Background: ATP-binding cassette sub-family B member 5 also known as P-glycoprotein ABCB5 is a plasma membrane-spanning protein that… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ABCB5, Polyclonal Antibody

Full Product Name

Anti-ABCB5 Antibody

Product Gene Name

anti-ABCB5 antibody

Product Synonym Names

ATP-binding cassette sub-family B member 5; ATP binding cassette subfamily B member 5; ATP-binding cassette sub-family B member 5; ABCB5 P-gp; P-glycoprotein ABCB5; ABCB5

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Lyophilized

Purity/Purification

Immunogen Affinity Purified

Specificity

No cross reactivity with other proteins.

Preparation and Storage

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

OMIM

611785

3D Structure

ModBase 3D Structure for Q2M3G0

Immunogen

E Coli-derived human ABCB5 recombinant protein (Position: M1-D176).

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Relevant Detection Systems

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC(P), IHC(F) and ICC.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500mug/ml.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-ABCB5 antibody

Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Direct ELISA (EIA)

Application Notes for anti-ABCB5 antibody

WB: 0.25-0.5ug/ml
IHC-P: 0.5-1ug/ml
IHC-F: 0.5-1ug/ml
ICC: 0.5-1ug/ml
FC/FACS: 1-3mug/1x106
Direct ELISA: 0.1-0.5ug/ml

Product References and Citations for anti-ABCB5 antibody

1. Chen, K. G., Szakacs, G., Annereau, J.-P., Rouzaud, F., Liang, X.-J., Valencia, J. C., Nagineni, C. N., Hooks, J. J., Hearing, V. J., Gottesman, M. M. Principal expression of two mRNA isoforms (ABCB5-alpha and ABCB5-beta) of the ATP-binding cassette transporter gene ABCB5 in melanoma cells and melanocytes. Pigment Cell Res. 18: 102-112, 2005. 2. Frank, N. Y., Margaryan, A., Huang, Y., Schatton, T., Waaga-Gasser, A. M., Gasser, M., Sayegh, M. H., Sadee, W., Frank, M. H. ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma. Cancer Res. 65: 4320-4333, 2005.

Other Notes

Small volumes of anti-ABCB5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ABCB5 purchase

MBS1752501 is an antibody targeting ABCB5. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ABCB5. It may not necessarily be applicable to this product.

NCBI GI #

255708477

NCBI GeneID

340273

NCBI Accession #

NP_001157413.1

UniProt Primary Accession #

Q2M3G0

UniProt Related Accession #

Q2M3G0

GenBank Nucleotide #

NP_001157413.1

NCBI Official Full Name

ATP-binding cassette sub-family B member 5 isoform 1

NCBI Official Synonym Full Names

ATP binding cassette subfamily B member 5

NCBI Official Symbol

ABCB5

NCBI Official Synonym Symbols

ABCB5beta; EST422562; ABCB5alpha

UniProt Protein Name

ATP-binding cassette sub-family B member 5

UniProt Synonym Protein Names

ABCB5 P-gp; P-glycoprotein ABCB5

Protein Family

ABC transporter B family

UniProt Gene Name

ABCB5

UniProt Entry Name

ABCB5_HUMAN

NCBI Protein Information

ATP-binding cassette sub-family B member 5

NCBI Summary for ABCB5

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]

UniProt Comments for ABCB5

ABCB5: Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, ABC family; Transporter

Chromosomal Location of Human Ortholog: 7p21.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: efflux transmembrane transporter activity; ATPase activity, coupled to transmembrane movement of substances; ATP binding

Biological Process: regulation of membrane potential; metabolic process; compound eye corneal lens development; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; cell differentiation; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; transmembrane transport; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent

Research Articles on ABCB5

1. High ABCB5 expression is associated with tumor cell invasiveness in colorectal cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ABCB5 antibody

Products by Pathway	Pathway Diagram
ABC Transporters Pathway antibodies	ABC Transporters Pathway Diagram
ABC Transporters Pathway antibodies	ABC Transporters Pathway Diagram
ABC-family Proteins Mediated Transport Pathway antibodies	ABC-family Proteins Mediated Transport Pathway Diagram
Transmembrane Transport Of Small Molecules Pathway antibodies	Transmembrane Transport Of Small Molecules Pathway Diagram

Diseases associated with anti-ABCB5 antibody

Disease Name	Pubmed Publications
Melanoma Antibodies	>58 publications with ABCB5 and Melanoma
Skin Neoplasms Antibodies	>20 publications with ABCB5 and Skin Neoplasms
Leukemia Antibodies	>7 publications with ABCB5 and Leukemia
Leukemia, Myeloid, Acute Antibodies	>5 publications with ABCB5 and Leukemia, Myeloid, Acute
Cell Transformation, Neoplastic Antibodies	>5 publications with ABCB5 and Cell Transformation, Neoplastic
Disease Models, Animal Antibodies	>3 publications with ABCB5 and Disease Models, Animal
Neoplasms, Experimental Antibodies	>3 publications with ABCB5 and Neoplasms, Experimental
Lung Neoplasms Antibodies	>2 publications with ABCB5 and Lung Neoplasms
Necrosis Antibodies	>2 publications with ABCB5 and Necrosis
Inflammation Antibodies	>2 publications with ABCB5 and Inflammation

Organs/Tissues associated with anti-ABCB5 antibody

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>27 publications with ABCB5 and Skin
Testis Antibodies	>3 publications with ABCB5 and Testis
Intestine Antibodies	>2 publications with ABCB5 and Intestine
Brain Antibodies	>2 publications with ABCB5 and Brain
Kidney Antibodies	>1 publications with ABCB5 and Kidney