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ALKBH5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753079

Description: Rabbit IgG polyclonal antibody for ALKBH5 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Monkey.Background: RNA demethylase ALKBH5 is an enzyme that in humans is encoded by the ALKBH5 gene. It is mapped to 17p11.2.… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ALKBH5, Polyclonal Antibody
Full Product Name
Anti-ALKBH5 Antibody
Product Gene Name
Product Synonym Names
RNA demethylase ALKBH5; Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5; ALKBH5; ABH5; OFOXD1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Monkey
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
OMIM
AK000315 mRNA
3D Structure
ModBase 3D Structure for Q6P6C2
Immunogen
E Coli-derived human ALKBH5 recombinant protein (Position: E81-R180).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ALKBH5 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes for anti-ALKBH5 antibody
WB: 0.1-0.25ug/ml (Human, Monkey)
IHC-P (Embedded Section): 0.5-1ug/ml (Human)
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.

Product References and Citations for anti-ALKBH5 antibody

1. Hartz, P. A. Personal Communication. Baltimore, Md. 3/12/2010.
2. Tsujikawa, K., Koike, K., Kitae, K., Shinkawa, A., Arima, H., Suzuki, T., Tsuchiya, M., Makino, Y., Furukawa, T., Konishi, N., Yamamoto, H. Expression and sub-cellular localization of human ABH family molecules. J. Cell. Molec. Med. 11: 1105-1116, 2007.
3. Zheng, G., Dahl, J. A., Niu, Y., Fedorcsak, P., Huang, C.-M., Li, C. J., Vagbo, C. B., Shi, Y., Wang, W.-L., Song, S.-H., Lu, Z., Bosmans, R. P. G., and 16 others. ALKBH5 is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility. Molec. Cell 49: 18-29, 2013.

Other Notes

Small volumes of anti-ALKBH5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ALKBH5 purchase

MBS1753079 is an antibody targeting ALKBH5. Reactive in Human, Monkey. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ALKBH5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
RNA demethylase ALKBH5
NCBI Official Synonym Full Names
alkB homolog 5, RNA demethylase
NCBI Official Symbol
ALKBH5
NCBI Official Synonym Symbols
ABH5; OFOXD; OFOXD1
UniProt Protein Name
RNA demethylase ALKBH5
UniProt Synonym Protein Names
Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5
Protein Family
UniProt Gene Name
ALKBH5
UniProt Synonym Gene Names
ABH5; OFOXD1
NCBI Protein Information
RNA demethylase ALKBH5

UniProt Comments for ALKBH5

ALKBH5: a protein contaning a 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily domain. May be involved in DNA alkylation damage repair

Protein type: DNA repair, damage; EC 1.14.11.-; Membrane protein, integral; Oxidoreductase; RNA-binding

Chromosomal Location of Human Ortholog: 17p11.2|17p11.2

Cellular Component: cytosol; Golgi apparatus; nuclear speck; nucleoplasm; nucleus

Molecular Function: metal ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; RNA binding

Biological Process: cell differentiation; DNA dealkylation involved in DNA repair; mRNA export from nucleus; mRNA processing; response to hypoxia; spermatogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-ALKBH5 antibody

Organs/Tissues associated with anti-ALKBH5 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >1 publications with ALKBH5 and Testis
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