Rabbit Anti-SETD1A Polyclonal Antibody – Cat# MBS1753091

Hset1/SET1/SETD1A (SETD1A) Antibody – Rabbit Polyclonal

Name: SETD1A Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1753091
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753091

Description: Rabbit IgG polyclonal antibody for hSET1/SET1/SETD1A detection. Tested with WB, IHC-P, IF, Direct ELISA in Human; Mouse; Rat.Background: Histone-lysine N-methyltransferase SETD1A is an enzyme that in humans is encoded by the SETD1A gene. It is… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

hSET1/SET1/SETD1A, Polyclonal Antibody

Full Product Name

Anti-hSET1/SET1/SETD1A Antibody

Product Gene Name

anti-SETD1A antibody

Product Synonym Names

Histone-lysine N-methyltransferase SETD1A; Lysine N-methyltransferase 2F; SET domain-containing protein 1A; Hset1a; Set1/Ash2 histone methyltransferase complex subunit SET1; SETD1A; KIAA0339; KMT2F; SET1; SET1A

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purity/Purification

Immunogen affinity purified.

Specificity

No cross reactivity with other proteins.

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

OMIM

AB002337 mRNA

3D Structure

ModBase 3D Structure for O15047

Immunogen

E Coli-derived human hSET1/SET1/SETD1A recombinant protein (Position: D8-K153).

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-SETD1A antibody

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Direct ELISA (EIA)

Application Notes for anti-SETD1A antibody

WB: 0.25-0.5ug/ml (Human)
IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse, Rat)
IF: 2ug/ml (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.

Product References and Citations for anti-SETD1A antibody

1. Guipponi, M., Santoni, F. A., Setola, V., Gehrig, C., Rotharmel, M., Cuenca, M., Guillin, O., Dikeos, D., Georgantopoulos, G., Papadimitrou, G., Curtis, L., Meary, A., and 9 others. Exome sequencing in 53 sporadic cases of schizophrenia identified 18 putative candidate genes. PLoS One 9: e112745, 2014. Note: Electronic Article. Erratum: PLoS One: 10: e0141630, 2015.
2. Lee, J.-H., Skalnik, D. G. CpG-binding protein (CXXC finger protein 1) is a component of the mammalian Set1 histone H3-Lys4 methyltransferase complex, the analogue of the yeast Set1/COMPASS complex. J. Biol. Chem. 280: 41725-41731, 2005.
3. Li, Y., Han, J., Zhang, Y., Cao, F., Liu, Z., Li, S., Wu, J., Hu, C., Wang, Y., Shuai, J., Chen, J., Cao, L., Li, D., Shi, P., Tian, C., Zhang, J., Dou, Y., Li, G., Chen, Y., Lei, M. Structural basis for activity regulation of MLL family methyltransferases. Nature 530: 447-452, 2016.

Other Notes

Small volumes of anti-SETD1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SETD1A purchase

MBS1753091 is an antibody targeting SETD1A. Also known as Anti-hSET1/SET1/SETD1A Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SETD1A. It may not necessarily be applicable to this product.

NCBI GI #

749385041

NCBI GeneID

9739

NCBI Accession #

NM_014712.2

UniProt Primary Accession #

O15047

UniProt Related Accession #

O15047

GenBank Nucleotide #

NM_014712.2

NCBI Official Full Name

Homo sapiens SET domain containing 1A (SETD1A), mRNA

NCBI Official Synonym Full Names

SET domain containing 1A, histone lysine methyltransferase

NCBI Official Symbol

SETD1A

NCBI Official Synonym Symbols

Set1; EPEDD; KMT2F; Set1A

UniProt Protein Name

Histone-lysine N-methyltransferase SETD1A

UniProt Synonym Protein Names

Lysine N-methyltransferase 2F; SET domain-containing protein 1A; hSET1A; Set1/Ash2 histone methyltransferase complex subunit SET1

Protein Family

Histone-lysine N-methyltransferase

UniProt Gene Name

SETD1A

UniProt Synonym Gene Names

; hSET1A

NCBI Protein Information

histone-lysine N-methyltransferase SETD1A

NCBI Summary for SETD1A

The protein encoded by this gene is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to generally mark the transcription start sites of active genes. The protein contains SET domains, a RNA recognition motif domain and is a member of the class V-like SAM-binding methyltransferase superfamily. [provided by RefSeq, Dec 2016]

UniProt Comments for SETD1A

SETD1A: Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys- 9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.

Protein type: Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: histone methyltransferase complex; nuclear chromatin; nuclear speck; nucleoplasm; nucleus

Molecular Function: beta-catenin binding; histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; protein binding; transcription factor binding

Research Articles on SETD1A

1. Results found that SETD1A is highly regulated in the poor outcome triple negative breast cancer (TNBC) cell line and may epigenetically regulate miR205 in TNBCs.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.