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AKR1C3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1754991

Background: Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
AKR1C3, Polyclonal Antibody
Full Product Name
Anti-AKR1C3 Antibody Picoband
Product Gene Name
anti-AKR1C3 antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Assay Type
Sandwich
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<15 pg/ml
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (lot specific)
Specificity
Natural and recombinant Rat Col18a1
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Molecular Weight
323
OMIM
603966
3D Structure
ModBase 3D Structure for P42330

Product Categories/Family for anti-AKR1C3 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-AKR1C3 antibody
Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-AKR1C3 antibody
WB: 0.25-0.5 ug/ml, Human
FC/FACS: 1-3 ug/1x10^6 cells, Human
Tested Species: In-house tested species with positive results.
Enhanced Chemiluminescent Kit with anti-Rabbit IgG (#MBS176460) for Western blot.

Western Blot (WB) of anti-AKR1C3 antibody

Figure 1. Western blot analysis of AKR1C3 using anti-AKR1C3 antibody (MBS1754991).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.

Lane 1: human HCCP tissue lysates,
Lane 2: human A549 whole cell lysates.

After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1C3 antigen affinity purified polyclonal antibody (#MBS1754991) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AKR1C3 at approximately 37 kDa. The expected band size for AKR1C3 is at 37 kDa.
Rabbit Anti-AKR1C3 Polyclonal Antibody – Cat# MBS1754991

Flow Cytometry (FC/FACS) of anti-AKR1C3 antibody

Figure 2. Flow Cytometry analysis of HepG2 cells using anti-AKR1C3 antibody (MBS1754991).
Overlay histogram showing HepG2 cells stained with MBS1754991 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AKR1C3 Antibody (MBS1754991,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-AKR1C3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1754991

Other Notes

Small volumes of anti-AKR1C3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AKR1C3 purchase

MBS1754991 is an antibody targeting AKR1C3. Also known as Anti-AKR1C3 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AKR1C3. It may not necessarily be applicable to this product.

NCBI GI #
308153646
NCBI GeneID
8644
NCBI Accession #
P42330.4
UniProt Primary Accession #
P42330
UniProt Secondary Accession #
Q5T2L1; Q96DJ1; Q96KI8; Q99530; Q9UCX1; Q9UII3; Q9UKL9; A8K2V0; B4DL37
UniProt Related Accession #
P42330
NCBI Official Full Name
Aldo-keto reductase family 1 member C3
NCBI Official Synonym Full Names
aldo-keto reductase family 1, member C3
NCBI Official Symbol
AKR1C3
NCBI Official Synonym Symbols
DD3; DDX; PGFS; HAKRB; HAKRe; HA1753; HSD17B5; hluPGFS
UniProt Protein Name
Aldo-keto reductase family 1 member C3
UniProt Synonym Protein Names
17-beta-hydroxysteroid dehydrogenase type 5; 17-beta-HSD 5; 3-alpha-HSD type II, brain; 3-alpha-hydroxysteroid dehydrogenase type 2 (EC:1.1.1.357); 3-alpha-HSD type 2; Chlordecone reductase homolog HAKRb; Dihydrodiol dehydrogenase 3; DD-3; DD3; Dihydrodiol dehydrogenase type I; HA1753; Indanol dehydrogenase (EC:1.1.1.112); Prostaglandin F synthase (EC:1.1.1.188); PGFS; Testosterone 17-beta-dehydrogenase 5 (EC:1.1.1.239, EC:1.1.1.64); Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase (EC:1.3.1.20)
Protein Family
Aldo-keto reductase family
UniProt Gene Name
AKR1C3
UniProt Synonym Gene Names
DDH1; HSD17B5; KIAA0119; PGFS; 17-beta-HSD 5; 3-alpha-HSD type 2; DD-3; DD3; PGFS
UniProt Entry Name
AK1C3_HUMAN
NCBI Protein Information
aldo-keto reductase family 1 member C3; indanol dehydrogenase; prostaglandin F synthase; 3-alpha-HSD type II, brain; dihydrodiol dehydrogenase 3; dihydrodiol dehydrogenase X; chlordecone reductase homolog HAKRb; testosterone 17-beta-dehydrogenase 5; 3-alp

NCBI Summary for AKR1C3

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
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UniProt Comments for AKR1C3

AKR1C3: Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta- PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone. Belongs to the aldo/keto reductase family.

Protein type: Lipid Metabolism - arachidonic acid; Oxidoreductase; EC 1.1.1.149; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: cytoplasm; intracellular; nucleus; cytosol

Molecular Function: 15-hydroxyprostaglandin-D dehydrogenase (NADP+) activity; delta4-3-oxosteroid 5beta-reductase activity; aldo-keto reductase activity; prostaglandin-F synthase activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity; 3(or 17)-alpha-hydroxysteroid dehydrogenase activity; retinol dehydrogenase activity; phenanthrene 9,10-monooxygenase activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; testosterone 17-beta-dehydrogenase (NADP+) activity; ketosteroid monooxygenase activity; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; ketoreductase activity; indanol dehydrogenase activity; aldehyde reductase activity; geranylgeranyl reductase activity; retinal dehydrogenase activity

Biological Process: steroid metabolic process; phototransduction, visible light; retinal metabolic process; cyclooxygenase pathway; male gonad development; progesterone metabolic process; cellular response to starvation; prostaglandin metabolic process; keratinocyte differentiation; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; protein import into nucleus, translocation; positive regulation of cell proliferation; arachidonic acid metabolic process; multicellular organismal macromolecule metabolic process; farnesol catabolic process; retinoid metabolic process; regulation of retinoic acid receptor signaling pathway; response to nutrient
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Research Articles on AKR1C3

1. Data suggest that reduction of daunorubicin/idarubicin is catalyzed by AKR1C3 and contributes to resistance of carcinoma cells to these anthracyclines; expression of AKR1C3 is induced in carcinoma cells following exposure to daunorubicin/idarubicin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-AKR1C3 antibody

 Products by Pathway  Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Benzo(a)pyrene Metabolism Pathway antibodies  Benzo(a)pyrene Metabolism Pathway Diagram
 Bile Acid And Bile Salt Metabolism Pathway antibodies  Bile Acid And Bile Salt Metabolism Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram

Diseases associated with anti-AKR1C3 antibody

 Disease Name  Pubmed Publications
 Prostatic Neoplasms Antibodies  >43 publications with AKR1C3 and Prostatic Neoplasms
 Breast Neoplasms Antibodies  >29 publications with AKR1C3 and Breast Neoplasms
 Adenocarcinoma Antibodies  >15 publications with AKR1C3 and Adenocarcinoma
 Obesity Antibodies  >11 publications with AKR1C3 and Obesity
 Endometriosis Antibodies  >9 publications with AKR1C3 and Endometriosis
 Neoplasms, Experimental Antibodies  >9 publications with AKR1C3 and Neoplasms, Experimental
 Inflammation Antibodies  >8 publications with AKR1C3 and Inflammation
 Gastrointestinal Diseases Antibodies  >8 publications with AKR1C3 and Gastrointestinal Diseases
 Hyperplasia Antibodies  >7 publications with AKR1C3 and Hyperplasia
 Polycystic Ovary Syndrome Antibodies  >6 publications with AKR1C3 and Polycystic Ovary Syndrome

Organs/Tissues associated with anti-AKR1C3 antibody

 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >87 publications with AKR1C3 and Prostate
 Blood Antibodies  >31 publications with AKR1C3 and Blood
 Liver Antibodies  >27 publications with AKR1C3 and Liver
 Lung Antibodies  >23 publications with AKR1C3 and Lung
 Ovary Antibodies  >17 publications with AKR1C3 and Ovary
 Adipose Tissue Antibodies  >16 publications with AKR1C3 and Adipose Tissue
 Uterus Antibodies  >14 publications with AKR1C3 and Uterus
 Connective Tissue Antibodies  >13 publications with AKR1C3 and Connective Tissue
 Skin Antibodies  >12 publications with AKR1C3 and Skin
 Adrenal Gland Antibodies  >12 publications with AKR1C3 and Adrenal Gland
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