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TRF1/TERF1 (TERF1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1755094

Telomeric repeat-binding factor 1 is a protein that in humans is encoded by the TERF1 gene. This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TRF1/TERF1, Polyclonal Antibody
Full Product Name
Anti-TRF1/TERF1 Antibody Picoband
Product Gene Name
anti-TERF1 antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
Molecular Weight
50,246 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (73921097..73959987). Location: 8q21.11
OMIM
600951
3D Structure
ModBase 3D Structure for P54274
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived human TRF1/TERF1 recombinant protein (Position: Q119-R341).

Product Categories/Family for anti-TERF1 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TERF1 antibody
ELISA (EIA), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-TERF1 antibody
WB: 0.25-0.5 ug/ml, Human
ICC/IF: 5 ug/ml, Human
Direct ELISA: 0.1-0.5 ug/ml, Human
Tested Species: In-house tested species with positive results.
Enhanced Chemiluminescent Kit with anti-Rabbit IgG (#MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (#MBS176453) for ICC.

Western Blot (WB) of anti-TERF1 antibody

Figure 1. Western blot analysis of TRF1/TERF1 using anti-TRF1/TERF1 antibody (MBS1755094).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.

Lane 1: human Jurkat whole cell lysates.

After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRF1/TERF1 antigen affinity purified polyclonal antibody (#MBS1755094) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRF1/TERF1 at approximately 55 kDa. The expected band size for TRF1/TERF1 is at 50 kDa.
Rabbit Anti-TERF1 Polyclonal Antibody – Cat# MBS1755094

Immunofluorescence (IF) of anti-TERF1 antibody

Figure 2. IF analysis of TRF1/TERF1 using anti-TRF1/TERF1 antibody (MBS1755094).

TRF1/TERF1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-TRF1/TERF1 Antibody (MBS1755094) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG (MBS177405) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-TERF1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1755094

Other Notes

Small volumes of anti-TERF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TERF1 purchase

MBS1755094 is an antibody targeting TERF1. Also known as Anti-TRF1/TERF1 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TERF1. It may not necessarily be applicable to this product.

NCBI GI #
189409140
NCBI GeneID
7013
NCBI Accession #
NP_003209.2
UniProt Primary Accession #
P54274
UniProt Secondary Accession #
Q15553; Q8NHT6; Q93029; A7XP29
UniProt Related Accession #
P54274
GenBank Nucleotide #
NM_003218.3
NCBI Official Full Name
telomeric repeat-binding factor 1 isoform 2
NCBI Official Synonym Full Names
telomeric repeat binding factor (NIMA-interacting) 1
NCBI Official Symbol
TERF1
NCBI Official Synonym Symbols
TRF; PIN2; TRF1; TRBF1; t-TRF1; hTRF1-AS
UniProt Protein Name
Telomeric repeat-binding factor 1
UniProt Synonym Protein Names
NIMA-interacting protein 2; TTAGGG repeat-binding factor 1; Telomeric protein Pin2/TRF1
Protein Family
Telomeric repeat-binding factor
UniProt Gene Name
TERF1
UniProt Synonym Gene Names
PIN2; TRBF1; TRF; TRF1
UniProt Entry Name
TERF1_HUMAN
NCBI Protein Information
telomeric repeat-binding factor 1; NIMA-interacting protein 2; telomeric protein Pin2/TRF1; TTAGGG repeat-binding factor 1

NCBI Summary for TERF1

This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq, Jul 2008]
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UniProt Comments for TERF1

TRF1: a telomeric repeat binding factor. Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Involved in the regulation of the mitotic spindle. Expression is tightly regulated during the cell cycle; levels are low in G1 and S phase and increase during G2 phase and mitosis. Two splice variant isoforms have been described.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: nucleoplasm; chromosome, telomeric region; nuclear telomere cap complex; nuclear chromosome, telomeric region; cytoplasm; nucleolus; spindle; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; microtubule binding; telomeric DNA binding; double-stranded telomeric DNA binding; chromatin binding; DNA bending activity

Biological Process: response to drug; mitosis; positive regulation of mitosis; negative regulation of telomere maintenance via semi-conservative replication; positive regulation of apoptosis; positive regulation of microtubule polymerization; telomere maintenance via telomerase; positive regulation of mitotic cell cycle; negative regulation of telomerase activity; cell division; negative regulation of DNA replication; telomeric loop formation; mitotic cell cycle spindle assembly checkpoint; G2/M transition of mitotic cell cycle; age-dependent telomere shortening; telomere maintenance; negative regulation of telomere maintenance via telomerase; protein homooligomerization
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Research Articles on TERF1

1. Low TRF1 expression is associated with chronic lymphocytic leukemia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TERF1 antibody

 Reference Product  PubMed Publications
 POT1 antibody  >10 publications with TERF1 and POT1
 TERF2IP antibody  >5 publications with TERF1 and TERF2IP
 TNKS2 antibody  >4 publications with TERF1 and TNKS2
 TNKS antibody  >4 publications with TERF1 and TNKS
 TINF2 antibody  >4 publications with TERF1 and TINF2
 ATM antibody  >3 publications with TERF1 and ATM
 PINX1 antibody  >2 publications with TERF1 and PINX1

Pathways associated with anti-TERF1 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 Meiosis Pathway antibodies  Meiosis Pathway Diagram
 Meiotic Synapsis Pathway antibodies  Meiotic Synapsis Pathway Diagram
 Packaging Of Telomere Ends Pathway antibodies  Packaging Of Telomere Ends Pathway Diagram
 Regulation Of Telomerase Pathway antibodies  Regulation Of Telomerase Pathway Diagram
 Shelterin Complex Pathway antibodies  Shelterin Complex Pathway Diagram
 Telomere Maintenance Pathway antibodies  Telomere Maintenance Pathway Diagram

Diseases associated with anti-TERF1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >13 publications with TERF1 and Neoplasms
 Anemia Antibodies  >2 publications with TERF1 and Anemia
 Death Antibodies  >2 publications with TERF1 and Death
 Pigmentation Disorders Antibodies  >1 publications with TERF1 and Pigmentation Disorders
 Congenital Abnormalities Antibodies  >1 publications with TERF1 and Congenital Abnormalities
 Nervous System Diseases Antibodies  >1 publications with TERF1 and Nervous System Diseases
 Hyperplasia Antibodies  >1 publications with TERF1 and Hyperplasia
 Kidney Diseases Antibodies  >1 publications with TERF1 and Kidney Diseases
 Fibrosis Antibodies  >1 publications with TERF1 and Fibrosis

Organs/Tissues associated with anti-TERF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >6 publications with TERF1 and Blood
 Bone Antibodies  >4 publications with TERF1 and Bone
 Lung Antibodies  >4 publications with TERF1 and Lung
 Embryonic Tissue Antibodies  >4 publications with TERF1 and Embryonic Tissue
 Skin Antibodies  >3 publications with TERF1 and Skin
 Muscle Antibodies  >2 publications with TERF1 and Muscle
 Brain Antibodies  >2 publications with TERF1 and Brain
 Nerve Antibodies  >1 publications with TERF1 and Nerve
 Kidney Antibodies  >1 publications with TERF1 and Kidney
 Prostate Antibodies  >1 publications with TERF1 and Prostate
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