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NOX5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS176174

Description: Rabbit IgG polyclonal antibody for NADPH oxidase 5(NOX5) detection. Tested with WB, IHC-P, ICC in Human.Background: NOX5(Nadph Oxidase 5), also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. The International… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NOX5, Polyclonal Antibody
Full Product Name
Anti-NOX5 antibody
Product Gene Name
anti-NOX5 antibody
Product Synonym Names
NADPH oxidase 5; NADPH oxidase, EF-hand calcium binding domain 5; MGC149776 antibody; MGC149777 antibody; NADPH oxidase 5 antibody; NADPH oxidase EFhand calcium binding domain 5 antibody; NOX5 antibody; NOX5_HUMAN antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
83,109 Da
OMIM
606572
3D Structure
ModBase 3D Structure for Q96PH1
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NOX5 (747-765aa KVLKGHCEKFGFRFFQENF).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-NOX5 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
Application Notes for anti-NOX5 antibody
Western Blot: Concentration: 0.1-0.5ug/ml; Tested Species: Hu
IHC (Paraffin-embedded section): Concentration: 0.5-1ug/ml; Tested Species: Hu; Antigen Retrieval: By Heat.
ICC: Concentration: 0.5-1ug/ml; Tested Species: Hu
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections to 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.

Immunohistochemistry (IHC) of anti-NOX5 antibody

Anti-NOX5 antibody, MBS176174, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Rabbit Anti-NOX5 Polyclonal Antibody – Cat# MBS176174

Immunocytochemistry (ICC) of anti-NOX5 antibody

Anti-NOX5 antibody, MBS176174, ICC
ICC: HELA Cell
Rabbit Anti-NOX5 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS176174

Western Blot (WB) of anti-NOX5 antibody

Anti-NOX5 antibody, MBS176174, Western blotting
All lanes: Anti (MBS176174) at 0.5ug/ml
Lane 1: 22RV1 Whole Cell Lysate at 40ug
Lane 2: PANC Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 86KD
Observed bind size: 86KD
Rabbit Anti-NOX5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS176174

Other Notes

Small volumes of anti-NOX5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NOX5 purchase

MBS176174 is an antibody targeting NOX5. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NOX5. It may not necessarily be applicable to this product.

NCBI GI #
74717091
NCBI GeneID
79400
NCBI Accession #
Q96PH1.1
UniProt Primary Accession #
Q96PH1
UniProt Secondary Accession #
Q08AN2; Q08AN3; Q8TEQ1; Q8TER4; Q96PH2; Q96PJ8; Q96PJ9; Q9H6E0; Q9HAM8; B2RBJ4
UniProt Related Accession #
Q96PH1
NCBI Official Full Name
NADPH oxidase 5
NCBI Official Synonym Full Names
NADPH oxidase, EF-hand calcium binding domain 5
NCBI Official Symbol
NOX5
UniProt Protein Name
NADPH oxidase 5
Protein Family
NADPH oxidase
UniProt Gene Name
NOX5
UniProt Entry Name
NOX5_HUMAN
NCBI Protein Information
NADPH oxidase 5

NCBI Summary for NOX5

This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
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UniProt Comments for NOX5

NOX5: Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Oxidoreductase; EC 1.6.3.-; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 15q23

Cellular Component: endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; FAD binding; superoxide-generating NADPH oxidase activity; heme binding; calcium ion binding; hydrogen ion channel activity; NADP binding

Biological Process: cell proliferation; proton transport; regulation of fusion of sperm to egg plasma membrane; apoptosis; endothelial cell proliferation; cytokinesis; regulation of proton transport; angiogenesis; cytokine secretion; superoxide release
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Research Articles on NOX5

1. The study reveals that PKC-alpha is the primary isoform mediating the activation of Nox5.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NOX5 antibody

 Reference Product  PubMed Publications
 NOXA1 antibody  >7 publications with NOX5 and NOXA1
 NOXO1 antibody  >6 publications with NOX5 and NOXO1
 CYBA antibody  >3 publications with NOX5 and CYBA
 SUMO1 antibody  >1 publications with NOX5 and SUMO1
 CD22 antibody  >1 publications with NOX5 and CD22
 HVCN1 antibody  >1 publications with NOX5 and HVCN1

Diseases associated with anti-NOX5 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >27 publications with NOX5 and Cardiovascular Diseases
 Inflammation Antibodies  >22 publications with NOX5 and Inflammation
 Hypertension Antibodies  >16 publications with NOX5 and Hypertension
 Adenocarcinoma Antibodies  >13 publications with NOX5 and Adenocarcinoma
 Necrosis Antibodies  >8 publications with NOX5 and Necrosis
 Kidney Diseases Antibodies  >6 publications with NOX5 and Kidney Diseases
 Heart Diseases Antibodies  >5 publications with NOX5 and Heart Diseases
 Lung Diseases Antibodies  >4 publications with NOX5 and Lung Diseases
 Heart Failure Antibodies  >3 publications with NOX5 and Heart Failure
 Hyperglycemia Antibodies  >2 publications with NOX5 and Hyperglycemia

Organs/Tissues associated with anti-NOX5 antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >54 publications with NOX5 and Vascular
 Blood Antibodies  >24 publications with NOX5 and Blood
 Lung Antibodies  >12 publications with NOX5 and Lung
 Kidney Antibodies  >12 publications with NOX5 and Kidney
 Testis Antibodies  >6 publications with NOX5 and Testis
 Prostate Antibodies  >5 publications with NOX5 and Prostate
 Skin Antibodies  >2 publications with NOX5 and Skin
 Spleen Antibodies  >2 publications with NOX5 and Spleen
 Brain Antibodies  >2 publications with NOX5 and Brain
 Placenta Antibodies  >1 publications with NOX5 and Placenta
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