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Prolactin Receptor (PRLR) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS176232

Description: Rabbit IgG polyclonal antibody for Prolactin receptor(PRLR) detection. Tested with WB, IHC-P in Human, Mouse, Rat.Background: PRLR(Prolactin Receptor), is a cytokine receptor. By somatic cell hybrid analysis and by in situ hybridization, Arden et… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Prolactin Receptor (PRLR), Polyclonal Antibody
Full Product Name
Anti-Prolactin Receptor antibody
Product Gene Name
anti-PRLR antibody
Product Synonym Names
Prolactin receptor; prolactin receptor; AI987712 antibody; CLONE SPM213 antibody; CPRLP antibody; Delta 4-delta 7/11 truncated prolactin receptor antibody; Delta 4-SF1b truncated prolactin receptor antibody; hPRL receptor antibody; hPRLrI antibody; Lactogen receptor antibody; MGC105486 antibody; OPR antibody; OTTHUMP00000115998 antibody; Pr-1 antibody; Pr-3 antibody; PRL R antibody; PRL-R antibody; PRLR antibody; Prlr-rs1 antibody; PRLR_HUMAN antibody; Prolactin receptor a antibody; Prolactin receptor antibody; Prolactin receptor delta 7/11 antibody; RATPRLR antibody; Secreted prolactin binding protein antibody; Truncated testis-specific box 1-C prolactin receptor antibody; wu:fj65c07 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Reacts with: Human, Rat
Predicted to work with: Mouse
Form/Format
Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
3D Structure
ModBase 3D Structure for Q08501
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Prolactin Receptor (591-605aa QLGRLDYLDPTCFMH), identical to the related rat sequence, and different from the related human sequence by three amino acids.
Gene Name
Prolactin receptor
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PRLR antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-PRLR antibody
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Human, Rat
Predicted Species: Mouse

Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1 ug/ml
Tested Species: Human, Rat
Predicted Species: Ms
Antigen Retrieval: By Heat

Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-PRLR antibody

Figure 1. Western blot analysis of PRLR using anti- PRLR antibody (MBS176232).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- PRLR antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRLR at approximately 90KD. The expected band size for PRLR is at 70KD.
Rabbit Anti-PRLR Polyclonal Antibody – Cat# MBS176232

Immunohistochemistry (IHC) of anti-PRLR antibody

Anti-PRLR antibody, MBS176232, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Rabbit Anti-PRLR Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS176232

Immunohistochemistry (IHC) of anti-PRLR antibody

Anti-PRLR antibody, MBS176232, IHC(P)
IHC(P): Rat Testis Tissue
Rabbit Anti-PRLR Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS176232

Other Notes

Small volumes of anti-PRLR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRLR purchase

MBS176232 is an antibody targeting PRLR. Also known as Anti-Prolactin Receptor antibody. Reactive in Reacts with: Human, Rat
Predicted to work with: Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PRLR. It may not necessarily be applicable to this product.

NCBI GI #
730343
NCBI GeneID
19116
NCBI Accession #
Q08501.1
UniProt Primary Accession #
Q08501
UniProt Secondary Accession #
P15212; P15213; Q62099
UniProt Related Accession #
Q08501
NCBI Official Full Name
Prolactin receptor
NCBI Official Synonym Full Names
prolactin receptor
NCBI Official Symbol
Prlr
NCBI Official Synonym Symbols
Pr-1; Pr-3; AI987712; Prlr-rs1
UniProt Protein Name
Prolactin receptor
Protein Family
Prolactin receptor
UniProt Gene Name
Prlr
UniProt Synonym Gene Names
PRL-R
UniProt Entry Name
PRLR_MOUSE
NCBI Protein Information
prolactin receptor; PRL-R; prolactin receptor related sequence 1

UniProt Comments for PRLR

PRLR: receptor for the anterior pituitary hormone prolactin and placental lactogen I and II. Interacts with Cyclophilin A, differentially regulating various signaling pathways from the PrlR. Prolactin signaling is attenuated by threonine and tyrosine phosphorylation. Two alternative splice isoforms have been identified.

Protein type: Receptor, cytokine; Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; intracellular

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; metal ion binding; peptide hormone binding; protein kinase binding; prolactin receptor activity

Biological Process: lactation; regulation of cell adhesion; transmembrane receptor protein tyrosine kinase activation (dimerization); regulation of epithelial cell differentiation; JAK-STAT cascade
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Research Articles on PRLR

1. This study showed in knockout mice showed no effect of PRL and PRL-R gene ablation on heat and cold hyperalgesia in male mice, while heat hyperlgesia were reduced 3-72 h post-surgery in female PRL and PRL-R knockout mice

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PRLR antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Growth Hormone Receptor Signaling Pathway antibodies  Growth Hormone Receptor Signaling Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram

Diseases associated with anti-PRLR antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >82 publications with PRLR and Breast Neoplasms
 Carcinoma Antibodies  >42 publications with PRLR and Carcinoma
 Mammary Neoplasms, Animal Antibodies  >18 publications with PRLR and Mammary Neoplasms, Animal
 Neoplasms, Experimental Antibodies  >12 publications with PRLR and Neoplasms, Experimental
 Infertility, Female Antibodies  >11 publications with PRLR and Infertility, Female
 Liver Diseases Antibodies  >10 publications with PRLR and Liver Diseases
 Necrosis Antibodies  >8 publications with PRLR and Necrosis
 Mammary Neoplasms, Experimental Antibodies  >8 publications with PRLR and Mammary Neoplasms, Experimental
 Weight Gain Antibodies  >8 publications with PRLR and Weight Gain
 Inflammation Antibodies  >7 publications with PRLR and Inflammation

Organs/Tissues associated with anti-PRLR antibody

 Organ/Tissue Name  Pubmed Publications
 Mammary Gland Antibodies  >82 publications with PRLR and Mammary Gland
 Liver Antibodies  >58 publications with PRLR and Liver
 Brain Antibodies  >55 publications with PRLR and Brain
 Ovary Antibodies  >53 publications with PRLR and Ovary
 Kidney Antibodies  >43 publications with PRLR and Kidney
 Pituitary Gland Antibodies  >43 publications with PRLR and Pituitary Gland
 Embryonic Tissue Antibodies  >28 publications with PRLR and Embryonic Tissue
 Adipose Tissue Antibodies  >27 publications with PRLR and Adipose Tissue
 Connective Tissue Antibodies  >25 publications with PRLR and Connective Tissue
 Prostate Antibodies  >21 publications with PRLR and Prostate
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