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HRP Conjugated Streptavidin (HRP) Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS176633

Description: Streptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein… More details

$205 / 0.5 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HRP Conjugated Streptavidin, Protein
Full Product Name
HRP Conjugated Streptavidin
Form/Format
Concentrated, Liquid
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at 4°C for one year.
Contents
1 mg of peroxidase conjugated streptavidin; 0.01M PBS (pH 7.4), 50% glycerol
Labeling Method
Streptavidin is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK
Preparation of Dilutent Buffer
Use 0.01 M TBS or 0.01 M PBS to dilute:

Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g Na2HPO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Conjugation
Peroxidase
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for HRP Conjugated Streptavidin protein
Dot Blot, Western Blot (WB), ELISA (EIA)
Dot Blot: 0.25-0.5ug/mL (ECL Detection)
Western Blotting: 0.1-0.3ug/ml (ECL detection)
Western Blotting: 0.3-2ug/ml (DAB detection)
Direct ELISA: 0.025-0.05ug/ml (TMB detection).
Optimal working dilutions must be determined by end users.

Other Notes

Small volumes of HRP Conjugated Streptavidin protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HRP Conjugated Streptavidin purchase

MBS176633 is a protein targeting HRP Conjugated Streptavidin.
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