IGFBP7 ELISA Kit for Human

This human IGFBP7 (Insulin-like Growth Factor-Binding Protein 7) PicoKine ELISA kit detects IGFBP7, a regulator of insulin-like growth factor availability, in cell culture supernatants and serum.

Background: Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The gene was mapped to chromosome 4q12. The major function of the protein is the regulation of availability of insulin-like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers.

Principle of the Assay: The Picokine™ Human IGFBP7 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human IGFBP7 with a 96-well strip plate that is pre-coated with antibody specific for IGFBP7. The detection antibody is a biotinylated antibody specific for IGFBP7. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit includes Human IGFBP7 protein as standards. To measure Human IGFBP7, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human IGFBP7 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IGFBP7 in the sample.

Small volumes of IGFBP7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS177364 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IGFBP7, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IGFBP7. The ELISA analytical biochemical technique of the MBS177364 kit is based on IGFBP7 antibody-IGFBP7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGFBP7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGFBP7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).