SETD8 Recombinant Protein

Function: Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration. Ref.1 Ref.2 Ref.7 Ref.9 Ref.10 Ref.15 Ref.16 Ref.17

Catalytic activity: S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]. Ref.1 Ref.2 Ref.8

Subunit structure: Interacts with L3MBTL1. Ref.11

Subcellular location: Nucleus. Chromosome. Note: Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin. Ref.1 Ref.6

Developmental stage: Not detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level). Ref.6

Induction: By HCFC1 C-terminal chain, independently of HCFC1 N-terminal chain. Transiently induced by TGF-beta and during the cell cycle. Ref.7 Ref.15

Domain: Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.

Post-translational modification: Ubiquitinated and degraded by the DCX(DTL) complex

Probable. Ref.15

Sequence similarities: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily.Contains 1 SET domain.

Caution: It is uncertain whether Met-1 or Met-72 is the initiator.

Sequence caution: The sequence AAL40879.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.