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Arachidonate 12-Lipoxygenase, 12s-Type(12s-LOX/12s-Lipoxygenase) (ALOX12) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177437

Description: Rabbit IgG polyclonal antibody for Arachidonate 12-lipoxygenase, 12S-type(ALOX12) detection. Tested with WB in Human.Background: ALOX12, Arachidonate 12-lipoxygenase, is an enzyme that in humans is encoded by the ALOX12 gene. By fluorescence in… More details

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Product Overview

Online Datasheet in PDF
Product Name
Arachidonate 12-lipoxygenase, 12S-type(12S-LOX/12S-lipoxygenase) (ALOX12), Polyclonal Antibody
Full Product Name
Anti-12 Lipoxygenase antibody
Product Gene Name
anti-ALOX12 antibody
Product Synonym Names
12 LOX antibody; 12(S) lipoxygenase antibody; 12S LOX antibody; 12S-lipoxygenase antibody; 12S-LOX antibody; 12S-type antibody; ALOX12 antibody; Alox12L antibody; Alox15 antibody; Arachidonate 12 lipoxygenase antibody; Arachidonate 12 lipoxygenase, 12S type antibody; Arachidonate 12 lipoxygenase, leukocyte type antibody; Arachidonate 12-lipoxygenase antibody; EC1.13.11.31 antibody; LOG12 antibody; LOX12_HUMAN antibody; Platelet type lipoxygenase 12 antibody; Platelet-type lipoxygenase 12 antibody; arachidonate 12-lipoxygenase
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human. No cross reactivity with other proteins.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
75,694 Da
OMIM
phenotype 152391
3D Structure
ModBase 3D Structure for P18054
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ALOX12(609-628aa HHKEKYFSGPKPKAVLNQFR), different from the related rat sequence by four amino acids, and from the related mouse sequence by five amino acids.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ALOX12 antibody
Western Blot (WB)
Application Notes for anti-ALOX12 antibody
Western Blot: Concentration: 0.1-0.5mug/ml; Tested Species: Human

Western Blot (WB) of anti-ALOX12 antibody

Anti- 12 Lipoxygenase antibody, MBS177437, Western blotting
All lanes: Anti ANOX12 (MBS177437) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted bind size: 75KD
Observed bind size: 75KD
Rabbit Anti-ALOX12 Polyclonal Antibody – Cat# MBS177437

Other Notes

Small volumes of anti-ALOX12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ALOX12 purchase

MBS177437 is an antibody targeting ALOX12. Also known as Anti-12 Lipoxygenase antibody. Reactive in Human. No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ALOX12. It may not necessarily be applicable to this product.

NCBI GI #
154426292
NCBI GeneID
239
NCBI Accession #
NP_000688.2
UniProt Primary Accession #
P18054
UniProt Secondary Accession #
O95569; Q6ISF8; Q9UQM4
UniProt Related Accession #
P18054
GenBank Nucleotide #
NM_000697.2
NCBI Official Full Name
arachidonate 12-lipoxygenase, 12S-type
NCBI Official Synonym Full Names
arachidonate 12-lipoxygenase
NCBI Official Symbol
ALOX12
NCBI Official Synonym Symbols
LOG12; 12-LOX; 12S-LOX
UniProt Protein Name
Arachidonate 12-lipoxygenase, 12S-type
UniProt Synonym Protein Names
Lipoxin synthase 12-LO (EC:3.3.2.-); Platelet-type lipoxygenase 12
Protein Family
Arachidonate 12-lipoxygenase
UniProt Gene Name
ALOX12
UniProt Synonym Gene Names
12LO; LOG12; 12S-LOX; 12S-lipoxygenase
UniProt Entry Name
LOX12_HUMAN
NCBI Protein Information
arachidonate 12-lipoxygenase, 12S-type; platelet 12-LOX; 12S-lipoxygenase; lipoxin synthase 12-LO; platelet-type 12-lipoxygenase; platelet-type lipoxygenase 12

UniProt Comments for ALOX12

ALOX12: Oxygenase and 14,15-leukotriene A4 synthase activity. Belongs to the lipoxygenase family.

Protein type: Lipid Metabolism - arachidonic acid; EC 1.13.11.31; Oxidoreductase; Apoptosis

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: membrane; cytoplasm; cytosol; sarcolemma

Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; hepoxilin A3 synthase activity; arachidonate 12-lipoxygenase activity; protein binding; lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding

Biological Process: positive regulation of cell adhesion; positive regulation of smooth muscle cell proliferation; positive regulation of caspase activity; linoleic acid metabolic process; positive regulation of vasodilation; positive regulation of cell growth; positive regulation of angiogenesis; positive regulation of endothelial cell differentiation; positive regulation of mitochondrial depolarization; lipoxygenase pathway; positive regulation of cell proliferation; arachidonic acid metabolic process; fatty acid oxidation; hepoxilin biosynthetic process; cell motility; hepoxilin metabolic process; superoxide release; aging; positive regulation of cell migration; negative regulation of apoptosis
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Research Articles on ALOX12

1. There was no statistically significant difference in expression of ALOX12 mRNA in cholesteatoma vs. control tissue.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ALOX12 antibody

 Reference Product  PubMed Publications
 PTGS2 antibody  >11 publications with ALOX12 and PTGS2
 PTGS1 antibody  >7 publications with ALOX12 and PTGS1
 PLA2G4A antibody  >1 publications with ALOX12 and PLA2G4A

Pathways associated with anti-ALOX12 antibody

 Products by Pathway  Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Eicosanoid Synthesis Pathway antibodies  Eicosanoid Synthesis Pathway Diagram
 Inflammatory Mediator Regulation Of TRP Channels Pathway antibodies  Inflammatory Mediator Regulation Of TRP Channels Pathway Diagram
 Inflammatory Mediator Regulation Of TRP Channels Pathway antibodies  Inflammatory Mediator Regulation Of TRP Channels Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Serotonergic Synapse Pathway antibodies  Serotonergic Synapse Pathway Diagram

Diseases associated with anti-ALOX12 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >18 publications with ALOX12 and Inflammation
 Cardiovascular Diseases Antibodies  >16 publications with ALOX12 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >8 publications with ALOX12 and Nervous System Diseases
 Skin Diseases Antibodies  >7 publications with ALOX12 and Skin Diseases
 Brain Diseases Antibodies  >5 publications with ALOX12 and Brain Diseases
 Lung Diseases Antibodies  >4 publications with ALOX12 and Lung Diseases
 Leukemia Antibodies  >4 publications with ALOX12 and Leukemia
 Hypertension Antibodies  >3 publications with ALOX12 and Hypertension
 Liver Diseases Antibodies  >2 publications with ALOX12 and Liver Diseases
 Liver Neoplasms Antibodies  >2 publications with ALOX12 and Liver Neoplasms

Organs/Tissues associated with anti-ALOX12 antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >7 publications with ALOX12 and Skin
 Muscle Antibodies  >2 publications with ALOX12 and Muscle
 Lung Antibodies  >2 publications with ALOX12 and Lung
 Embryonic Tissue Antibodies  >2 publications with ALOX12 and Embryonic Tissue
 Ovary Antibodies  >1 publications with ALOX12 and Ovary
 Connective Tissue Antibodies  >1 publications with ALOX12 and Connective Tissue
 Intestine Antibodies  >1 publications with ALOX12 and Intestine
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