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Sodium/Hydrogen Exchanger 1 (SLC9A1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177483

Background: NHE1, also known as SLC9A1, is an isoform of sodium–hydrogen antiporter that in humans is encoded by the SLC9A1 gene. It is mapped to 1p36.11. NHE1 is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Sodium/hydrogen exchanger 1 (SLC9A1), Polyclonal Antibody
Full Product Name
Anti-SLC9A1 Antibody
Product Gene Name
anti-SLC9A1 antibody
Product Synonym Names
amiloride-sensitive antibody; APNH antibody; APNH1 antibody; FLJ42224 antibody; Na Li countertransporter antibody; Na(+)/H(+) antiporter antibody; Na(+)/H(+) exchanger 1 antibody; Na+ H+ antiporter amiloride-sensitive antibody; Na+ H+ antiporter antibody; Na+ H+ exchanger 1 antibody; NHE-1 antibody; NHE1 antibody; OTTHUMP00000004468 antibody; SL9A1_HUMAN antibody; SLC9A1 antibody; Sodium hydrogen exchanger 1 antibody; Sodium/hydrogen exchanger 1 antibody; solute carrier family 9 antibody; Solute carrier family 9 member 1 antibody; Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+ H+ amiloride sensitive antibody; Solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1 antibody; Solute carrier family 9 subfamily A member 1 antibody; solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with othee protiens
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
OMIM
107310
3D Structure
ModBase 3D Structure for P19634
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E.coli-derived human SLC9A1 recombinant protein (Position: H543-Q815). Human SLC9A1 shares 92% and 93% amino acid (aa) sequences identity with mouse and rat SLC9A1, respectively.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SLC9A1 antibody
Western Blot (WB), Immunohistochemistry (IHC-P)
Application Notes for anti-SLC9A1 antibody
Western Blot:
Concentration: 0.1-0.5ug/ml; Tested Species: Human, Rat

Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat

WB: The detection limit for SLC9A1 is approximately 0.25ng/lane under reducing conditions.
Tested Species: In house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer , pH 6.0 for 20mins is required for the staining of formalin/paraffin sections.

Other applications have not been tested
Optimal dilutions should be determined by the end users.

Testing Data of anti-SLC9A1 antibody

Anti-SLC9A1 Picoband antibody, MBS177483-1.jpg
All lanes: Anti SLC9A1 (MBS177483) at 0.5ug/ml
WB: Recombinant Human Sodium Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
Rabbit Anti-SLC9A1 Polyclonal Antibody – Cat# MBS177483

Testing Data of anti-SLC9A1 antibody

Anti-SLC9A1 Picoband antibody, MBS177483-2.jpg
All lanes: Anti SLC9A1 (MBS177483) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 91KD
Observed bind size: 91KD
Rabbit Anti-SLC9A1 Polyclonal Antibody, Testing Data – Cat# MBS177483

Immunohistochemistry (IHC) of anti-SLC9A1 antibody

Anti-SLC9A1 Picoband antibody, MBS177483-3.JPG
IHC(P): Rat Kidney Tissue
Rabbit Anti-SLC9A1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177483

Immunohistochemistry (IHC) of anti-SLC9A1 antibody

Anti-SLC9A1 Picoband antibody, MBS177483-4.JPG
IHC(P): Human Mammary Cancer Tissue
Rabbit Anti-SLC9A1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177483

Immunohistochemistry (IHC) of anti-SLC9A1 antibody

Anti-SLC9A1 Picoband antibody, MBS177483-5.JPG
IHC(P): Mouse Kidney Tissue
Rabbit Anti-SLC9A1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177483

Other Notes

Small volumes of anti-SLC9A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SLC9A1 purchase

MBS177483 is an antibody targeting SLC9A1. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SLC9A1. It may not necessarily be applicable to this product.

NCBI GI #
27777632
NCBI GeneID
6548
NCBI Accession #
NP_003038.2
UniProt Primary Accession #
P19634
UniProt Secondary Accession #
Q96EM2; B1ALD6; D3DPL4
UniProt Related Accession #
P19634
GenBank Nucleotide #
NM_003047.4
NCBI Official Full Name
sodium/hydrogen exchanger 1
NCBI Official Synonym Full Names
solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1
NCBI Official Symbol
SLC9A1
NCBI Official Synonym Symbols
APNH; NHE1; NHE-1; PPP1R143
UniProt Protein Name
Sodium/hydrogen exchanger 1
UniProt Synonym Protein Names
APNH; Na(+)/H(+) antiporter, amiloride-sensitive; Na(+)/H(+) exchanger 1; NHE-1; Solute carrier family 9 member 1
Protein Family
Sodium/hydrogen exchanger
UniProt Gene Name
SLC9A1
UniProt Synonym Gene Names
APNH1; NHE1; NHE-1
UniProt Entry Name
SL9A1_HUMAN
NCBI Protein Information
sodium/hydrogen exchanger 1; Na(+)/H(+) exchanger 1; Na-Li countertransporter; protein phosphatase 1, regulatory subunit 143; solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive); solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter, Na+/H+, amiloride sensitive)

NCBI Summary for SLC9A1

This gene encodes a Na+/H+ antiporter that is a member of the solute carrier family 9. The encoded protein is a plasma membrane transporter that is expressed in the kidney and intestine. This protein plays a central role in regulating pH homeostasis, cell migration and cell volume. This protein may also be involved in tumor growth. [provided by RefSeq, Sep 2011]
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UniProt Comments for SLC9A1

NHE1: sodium/hydrogen exchanger 1. Belongs to the Na(+)/H(+) exchanger family. Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient.

Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Transporter; Transporter, SLC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.1-p35

Cellular Component: endoplasmic reticulum membrane; cell surface; focal adhesion; mitochondrion; integral to plasma membrane; endoplasmic reticulum; basolateral plasma membrane; T-tubule; integral to membrane; lipid raft; nucleoplasm; lamellipodium; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; plasma membrane

Molecular Function: protein binding, bridging; calmodulin binding; protein binding; phosphatidylinositol-4,5-bisphosphate binding; protein complex scaffold; solute:hydrogen antiporter activity; calcium-dependent protein binding; sodium:hydrogen antiporter activity; protein phosphatase 2B binding

Biological Process: glycosaminoglycan metabolic process; positive regulation of NFAT protein import into nucleus; pathogenesis; cardiac muscle cell differentiation; hyaluronan catabolic process; regulation of pH; positive regulation of action potential; cell growth; transmembrane transport; response to drug; cellular sodium ion homeostasis; cell migration; maintenance of cell polarity; positive regulation of cell growth; protein oligomerization; cellular response to insulin stimulus; regulation of stress fiber formation; regulation of intracellular pH; carbohydrate metabolic process; regulation of focal adhesion formation; ion transport; positive regulation of transcription from RNA polymerase II promoter; regulation of sensory perception of pain; response to acidity; hyaluronan metabolic process; negative regulation of apoptosis

Disease: Lichtenstein-knorr Syndrome
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Research Articles on SLC9A1

1. CIAPIN1 targeted NHE1 to mediate differentiation of K562 cells via ERK1/2 pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SLC9A1 antibody

 Reference Product  PubMed Publications
 CHP2 antibody  >5 publications with SLC9A1 and CHP2
 CHP antibody  >5 publications with SLC9A1 and CHP
 EGFR antibody  >2 publications with SLC9A1 and EGFR
 CD44 antibody  >1 publications with SLC9A1 and CD44
 TESC antibody  >1 publications with SLC9A1 and TESC
 MAPK14 antibody  >1 publications with SLC9A1 and MAPK14

Pathways associated with anti-SLC9A1 antibody

 Products by Pathway  Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Defective B3GAT3 Causes JDSSDHD Pathway antibodies  Defective B3GAT3 Causes JDSSDHD Pathway Diagram
 Defective B4GALT1 Causes B4GALT1-CDG (CDG-2d) Pathway antibodies  Defective B4GALT1 Causes B4GALT1-CDG (CDG-2d) Pathway Diagram
 Defective B4GALT7 Causes EDS, Progeroid Type Pathway antibodies  Defective B4GALT7 Causes EDS, Progeroid Type Pathway Diagram
 Defective CHST14 Causes EDS, Musculocontractural Type Pathway antibodies  Defective CHST14 Causes EDS, Musculocontractural Type Pathway Diagram

Diseases associated with anti-SLC9A1 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >68 publications with SLC9A1 and Cardiovascular Diseases
 Heart Diseases Antibodies  >46 publications with SLC9A1 and Heart Diseases
 Reperfusion Injury Antibodies  >34 publications with SLC9A1 and Reperfusion Injury
 Hypertrophy Antibodies  >28 publications with SLC9A1 and Hypertrophy
 Disease Models, Animal Antibodies  >24 publications with SLC9A1 and Disease Models, Animal
 Nervous System Diseases Antibodies  >21 publications with SLC9A1 and Nervous System Diseases
 Cardiomyopathies Antibodies  >21 publications with SLC9A1 and Cardiomyopathies
 Heart Failure Antibodies  >20 publications with SLC9A1 and Heart Failure
 Hypertension Antibodies  >20 publications with SLC9A1 and Hypertension
 Cardiomegaly Antibodies  >17 publications with SLC9A1 and Cardiomegaly

Organs/Tissues associated with anti-SLC9A1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >89 publications with SLC9A1 and Muscle
 Blood Antibodies  >66 publications with SLC9A1 and Blood
 Kidney Antibodies  >44 publications with SLC9A1 and Kidney
 Brain Antibodies  >36 publications with SLC9A1 and Brain
 Vascular Antibodies  >28 publications with SLC9A1 and Vascular
 Liver Antibodies  >20 publications with SLC9A1 and Liver
 Intestine Antibodies  >15 publications with SLC9A1 and Intestine
 Mouth Antibodies  >8 publications with SLC9A1 and Mouth
 Lung Antibodies  >8 publications with SLC9A1 and Lung
 Skin Antibodies  >7 publications with SLC9A1 and Skin
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