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Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177548

Description: Sandwich ELISA kit of Quantitative Detection for Human TFPI2Background: TFPI2, also called Tissue factor pathway inhibitor 2, is a protein that in humans is encoded by the TFPI2 gene. It is mapped to 7q21.3. This gene encodes a member of the… More details

$635 / 96-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Tissue factor pathway inhibitor 2 (TFPI2), ELISA Kit
Full Product Name
Human TFPI2 PicoKine ELISA Kit
Product Gene Name
Product Synonym Names
Placental protein 5; PP5; TFPI 2; TFPI-2; TFPI2; TFPI2 PEN; TFPI2_HUMAN; Tissue factor pathway inhibitor 2; tissue factor pathway inhibitor 2
Species Reactivity
Human. There is no detectable cross-reactivity with other relevant proteins.
Assay Type
Sandwich
Samples
Cell culture supernates and serum
Sensitivity
<10pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Molecular Weight
25,796 Da
OMIM
600033
3D Structure
ModBase 3D Structure for P48307
Production Time
Due to severe delay and raw materials shortage, production time may takes between 5-8 weeks.
Immunogen
NSO, D23-F235
Intra-Assay Precision
Sample 1: n=16; Mean(ng/ml): 1.75; Standard deviation: 0.074; CV(%): 4.2 Sample 2: n=16; Mean(ng/ml): 3.28; Standard deviation: 0.154; CV(%): 4.7 Sample 3: n=16; Mean(ng/ml): 6.32; Standard deviation: 0.354; CV(%): 5.6
Inter-Assay Precision
Sample 1: n=24; Mean(ng/ml): 1.64; Standard deviation: 0.08; CV(%): 4.9 Sample 2: n=24; Mean(ng/ml): 3.09; Standard deviation: 0.164; CV(%): 5.3 Sample 3: n=24; Mean(ng/ml): 6.47; Standard deviation: 0.395; CV(%): 6.1
Assay Range
156pg/ml-10,000pg/ml
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of TFPI2 elisa kit

Human TFPI2 PicoKine ELISA Kit standard curve
Human TFPI2 ELISA Kit – Cat# MBS177548

Other Notes

Small volumes of TFPI2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TFPI2 purchase

MBS177548 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tissue factor pathway inhibitor 2 (TFPI2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TFPI2. The ELISA analytical biochemical technique of the MBS177548 kit is based on TFPI2 antibody-TFPI2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TFPI2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TFPI2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TFPI2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tissue factor pathway inhibitor 2 isoform 2
NCBI Official Synonym Full Names
tissue factor pathway inhibitor 2
NCBI Official Symbol
TFPI2
NCBI Official Synonym Symbols
PP5; REF1; TFPI-2
UniProt Protein Name
Tissue factor pathway inhibitor 2
UniProt Synonym Protein Names
Placental protein 5; PP5
UniProt Gene Name
TFPI2
UniProt Synonym Gene Names
TFPI-2; PP5
UniProt Entry Name
TFPI2_HUMAN
NCBI Protein Information
tissue factor pathway inhibitor 2; placental protein 5; retinal pigment epithelium cell factor 1

NCBI Summary for TFPI2

This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
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UniProt Comments for TFPI2

TFPI2: May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: proteinaceous extracellular matrix; nucleus

Molecular Function: serine-type endopeptidase inhibitor activity; extracellular matrix structural constituent

Biological Process: blood coagulation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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