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Cripto/TDGF1 Picokine (TDGF1) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177640

Principle of the assay: Mouse Cripto ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cripto has been precoated onto 96-well plates. Standards (NSO, R26-Q150) and test samples… More details

$635 / 96-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cripto/TDGF1 PicoKine, ELISA Kit
Full Product Name
Mouse Cripto/TDGF1 PicoKine ELISA Kit
Product Gene Name
Tdgf1 elisa kit
Product Synonym Names
Teratocarcinoma-derived growth factor; CR; CRGF; Cripto 1; Cripto 1 growth factor; cripto; Cripto-1 growth factor; Cripto1; Cripto1 growth factor; Epidermal growth factor like cripto protein CR1; Epidermal growth factor-like cripto protein CR1; TDGF 1; TDGF1; TDGF1_HUMAN; Teratocarcinoma derived growth factor 1; Teratocarcinoma-derived growth factor 1 ; teratocarcinoma-derived growth factor 1
Species Reactivity
Mouse
Sensitivity
<10pg/ml
Specificity
Natural and recombinant mouse Cripto
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Molecular Weight
18,754 Da
3D Structure
ModBase 3D Structure for P51865
Production Time
Due to severe delay and raw materials shortage, production time may takes between 5-8 weeks.
Assay Range
62.5pg/ml-4000pg/ml
Sample
For quantitative detection of mouse Cripto in cell culture supernates, serum and plasma(heparin, EDTA).
Immunogen
NSO, R26-Q150
Assay Procedure Step 1
Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse Cripto standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse Cripto standard solution and each sample is measured in duplicate.
Intra-Assay Precision
Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|675|1810|2944|792|1948|3107
Standard deviation|34.42|108.6|197.24|43.56|140.25|211.27
CV(%)|5.1|6.0|6.7|5.5|7.2|6.8
Sample Data
Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000
O.D.|0.079|0.138|0.181|0.283|0.446|0.764|1.354|2.050
Anticoagulant
Heparin or EDTA
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of Tdgf1 elisa kit

Mouse Cripto/TDGF1 PicoKine ELISA Kit standard curve
Mouse TDGF1 ELISA Kit – Cat# MBS177640

Other Notes

Small volumes of Tdgf1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Tdgf1 purchase

MBS177640 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cripto/TDGF1 PicoKine, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Tdgf1. The ELISA analytical biochemical technique of the MBS177640 kit is based on Tdgf1 antibody-Tdgf1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Tdgf1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Tdgf1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Tdgf1. It may not necessarily be applicable to this product.

NCBI GI #
1706128
NCBI GeneID
21667
NCBI Accession #
P51865.1
UniProt Primary Accession #
P51865
UniProt Related Accession #
P51865
NCBI Official Full Name
Teratocarcinoma-derived growth factor
NCBI Official Synonym Full Names
teratocarcinoma-derived growth factor 1
NCBI Official Symbol
Tdgf1
NCBI Official Synonym Symbols
CR1; cripto
UniProt Protein Name
Teratocarcinoma-derived growth factor
UniProt Synonym Protein Names
Cripto growth factor; Epidermal growth factor-like Cripto protein
Protein Family
Teratocarcinoma-derived growth factor
UniProt Gene Name
Tdgf1
UniProt Synonym Gene Names
Cripto
UniProt Entry Name
TDGF1_MOUSE
NCBI Protein Information
teratocarcinoma-derived growth factor

UniProt Comments for Tdgf1

TDGF1: Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. Interacts with the activin type-1 receptor ACVR1B. Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. Expressed in breast and lung.

Protein type: Cell adhesion; Cell cycle regulation; Motility/polarity/chemotaxis; Membrane protein, GPI anchor; Membrane protein, peripheral

Cellular Component: apical plasma membrane; cell surface; cytoplasm; extracellular region; extracellular space; extrinsic to plasma membrane; Golgi apparatus; lipid raft; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: coreceptor activity; growth factor activity; protein binding; receptor binding; Wnt-protein binding

Biological Process: activation of MAPK activity; anterior/posterior axis specification; BMP signaling pathway; cardiac muscle cell differentiation; cell differentiation; cell migration during sprouting angiogenesis; determination of anterior/posterior axis, embryo; epidermal growth factor receptor signaling pathway; gastrulation; heart development; in utero embryonic development; morphogenesis of a branching structure; negative regulation of apoptosis; negative regulation of transforming growth factor beta receptor signaling pathway; peptidyl-serine phosphorylation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of fibroblast proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; regulation of cell migration; regulation of signal transduction; vasculogenesis; Wnt receptor signaling pathway through beta-catenin
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Research Articles on Tdgf1

1. our findings strongly suggest that Cripto-1 is a potential driver of mammary tumorigenesis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Tdgf1 elisa kit

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 Lefty1 elisa kit  >5 publications with Tdgf1 and Lefty1
 Lefty2 elisa kit  >4 publications with Tdgf1 and Lefty2
 Acvr2b elisa kit  >1 publications with Tdgf1 and Acvr2b

Pathways associated with Tdgf1 elisa kit

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 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Regulation Of Signaling By NODAL Pathway antibodies  Regulation Of Signaling By NODAL Pathway Diagram
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Organs/Tissues associated with Tdgf1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >66 publications with Tdgf1 and Embryonic Tissue
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