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S100A9 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177733

Background: S100 calcium-binding protein A9 (S100A9), also known as migration inhibitory factor-related protein 14 (MRP14) or calgranulin B, is a protein that in humans is encoded by the S100A9 gene. S100-A9 is a member of the S100 family of proteins… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
S100A9, Polyclonal Antibody
Full Product Name
Anti-S100A9 Antibody
Product Gene Name
anti-S100A9 antibody
Product Synonym Names
Protein S100-A9; Leukocyte L1 complex heavy chain; 60B8AG; AW546964; BEE22; CAGB; Calgranulin B; Calgranulin-B; Calprotectin L1H subunit; CFAG; CGLB; Cystic fibrosis antigen; Cystic fibrosis antigen B; HGNC:10499; L1AG; Leukocyte L1 complex heavy chain; LIAG; MAC 387; MAC387; MIF; Migration inhibitory factor related protein 14; Migration inhibitory factor-related protein 14; MRP 14; MRP 8; MRP-14; MRP14; Myeloid-related protein 14; NIF; OTTHUMP00000015331; P14; Protein S100-A9; S100 A9; S100 calcium binding protein A9; S100 calcium binding protein A9 calgranulin B; S100 calcium-binding protein A9; S100A9; S10A9_HUMAN; S100 calcium binding protein A9
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a 6 months.
Avoid repeated freezing and thawing.
3D Structure
ModBase 3D Structure for P31725
Immunogen
E Coli-derived mouse S100A9 recombinant protein (Position: A2-K113). Mouse S100A9 shares 59.8% and 78.6% amino acid (aa) sequence identity with human and rat S100A9, respectively.
Cross Reactivity
Cross-reactivity with recombinant human S100A9 is observed.
Reconstitution
0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detections Systems
The lab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (Please inquire) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (please inquire) for IHC(P) and ICC.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-S100A9 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-p), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC)
Application Notes for anti-S100A9 antibody
ELISA:
Concentration: 0.1-0.5ug/ml
Tested Species: Mouse
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Mouse
IHC-p
Concentration: 0.5-1ug/ml
Tested Species: Mouse , Rat
Antigen Retrieval: By Heat
ICC/IF:
Concentration: 5ug/mL
Tested Species: Mouse, Rat
Flow Cytometry:
Concentration: 1-3ug/1x106 cells
Tested Species: Mouse

Tested Species: In-house tested species with positive results
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.

Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-S100A9 antibody

Anti- S100A9 Picoband antibody, MBS177733, Western blotting
All lanes: Anti S100A9 (MBS177733) at 0.5ug/ml
WB: Mouse Ovary Tissue Lysate at 50ug
Predicted bind size: 13KD
Observed bind size: 13KD
Rabbit Anti-S100A9 Polyclonal Antibody – Cat# MBS177733

Immunohistochemistry (IHC) of anti-S100A9 antibody

Anti- S100A9 Picoband antibody, MBS177733, IHC(P)
IHC(P): Mouse Spleen Tissue
Rabbit Anti-S100A9 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177733

Immunohistochemistry (IHC) of anti-S100A9 antibody

Anti- S100A9 Picoband antibody, MBS177733, IHC(P)
IHC(P): Rat Spleen Tissue
Rabbit Anti-S100A9 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177733

Product References and Citations for anti-S100A9 antibody

1. "Entrez Gene: S100A9 S100 calcium binding protein A9". 2. Cheng P, Corzo CA, Luetteke N, Yu B, Nagaraj S, Bui MM, Ortiz M, Nacken W, Sorg C, Vogl T, Roth J, Gabrilovich DI (September 2008). "Inhibition of dendritic cell differentiation and accumulation of myeloid-derived suppressor cells in cancer is regulated by S100A9 protein". J. Exp. Med. 205 (10): 2235-49.

Other Notes

Small volumes of anti-S100A9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for S100A9 purchase

MBS177733 is an antibody targeting S100A9. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for S100A9. It may not necessarily be applicable to this product.

NCBI GI #
529367291
NCBI GeneID
20202
NCBI Accession #
NP_001268781.1
UniProt Primary Accession #
P31725
UniProt Related Accession #
P31725
GenBank Nucleotide #
NM_001281852.1
NCBI Official Full Name
protein S100-A9
NCBI Official Synonym Full Names
S100 calcium binding protein A9 (calgranulin B)
NCBI Official Symbol
S100a9
NCBI Official Synonym Symbols
p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964
UniProt Protein Name
Protein S100-A9
UniProt Synonym Protein Names
Calgranulin-B; Leukocyte L1 complex heavy chain; Migration inhibitory factor-related protein 14; MRP-14; p14; S100 calcium-binding protein A9
Protein Family
Protein
UniProt Gene Name
S100a9
UniProt Synonym Gene Names
Cagb; Mrp14; MRP-14; p14
UniProt Entry Name
S10A9_MOUSE
NCBI Protein Information
protein S100-A9

UniProt Comments for S100A9

S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner.

Protein type: Calcium-binding

Cellular Component: cytoplasm; cytoskeleton; extracellular region; extracellular space; membrane; nucleus; plasma membrane

Molecular Function: antioxidant activity; arachidonic acid binding; calcium ion binding; metal ion binding; microtubule binding; RAGE receptor binding; zinc ion binding

Biological Process: actin cytoskeleton reorganization; apoptosis; astrocyte development; autophagy; caspase activation; chemotaxis; immune system process; inflammatory response; innate immune response; leukocyte chemotaxis; leukocyte migration during inflammatory response; neutrophil chemotaxis; peptidyl-cysteine S-nitrosylation; positive regulation of blood coagulation; positive regulation of inflammatory response; positive regulation of peptide secretion; regulation of inflammatory response; regulation of integrin biosynthetic process; regulation of translation
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Research Articles on S100A9

1. Canonical Wnt signaling was decreased after induction of experimental OA in S100A9-deficient mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-S100A9 antibody

 Reference Product  PubMed Publications
 S100a8 antibody  >894 publications with S100A9 and S100a8
 Tlr4 antibody  >75 publications with S100A9 and Tlr4
 Camp antibody  >4 publications with S100A9 and Camp
 Ly96 antibody  >2 publications with S100A9 and Ly96
 Parp1 antibody  >2 publications with S100A9 and Parp1
 Ncf1 antibody  >1 publications with S100A9 and Ncf1
 Myb antibody  >1 publications with S100A9 and Myb

Diseases associated with anti-S100A9 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >466 publications with S100A9 and Inflammation
 Disease Models, Animal Antibodies  >125 publications with S100A9 and Disease Models, Animal
 Nervous System Diseases Antibodies  >90 publications with S100A9 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >88 publications with S100A9 and Cardiovascular Diseases
 Necrosis Antibodies  >85 publications with S100A9 and Necrosis
 Brain Diseases Antibodies  >55 publications with S100A9 and Brain Diseases
 Fibrosis Antibodies  >55 publications with S100A9 and Fibrosis
 Heart Diseases Antibodies  >37 publications with S100A9 and Heart Diseases
 Neoplasms, Experimental Antibodies  >37 publications with S100A9 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >33 publications with S100A9 and Breast Neoplasms

Organs/Tissues associated with anti-S100A9 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >532 publications with S100A9 and Blood
 Skin Antibodies  >126 publications with S100A9 and Skin
 Lung Antibodies  >120 publications with S100A9 and Lung
 Liver Antibodies  >59 publications with S100A9 and Liver
 Joint Antibodies  >55 publications with S100A9 and Joint
 Bone Marrow Antibodies  >48 publications with S100A9 and Bone Marrow
 Kidney Antibodies  >46 publications with S100A9 and Kidney
 Lymph Node Antibodies  >32 publications with S100A9 and Lymph Node
 Spleen Antibodies  >23 publications with S100A9 and Spleen
 Uterus Antibodies  >16 publications with S100A9 and Uterus
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