Anti-EED Polyclonal Antibody – Cat# MBS177817

Anti- EED Picoband antibody, MBS177817, Western blotting
All lanes: Anti EED (MBS177817) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Lung Tissue Lysate at 50ug
Lane 5: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 72KD

EED Antibody – Polyclonal

Name: EED Antibody; Polyclonal; Human/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS177817
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177817

Description: Rabbit IgG polyclonal antibody for Polycomb protein EED(EED) detection. Tested with WB in Human;Rat.Background: Polycomb protein EED is a protein that in humans is encoded by the EED gene. It is a member of the Polycomb-group (PcG) family. PcG… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

EED, Polyclonal Antibody

Full Product Name

Anti-EED Antibody

Product Gene Name

anti-EED antibody

Product Synonym Names

Polycomb protein EED; eed; EED protein; EED, mouse, homolog of; EED_HUMAN; Embryonic ectoderm development; Embryonic ectoderm development isoform a; Embryonic ECTODERM development protein homolog; Embryonic ectoderm development protein, mouse, homolog of; HEED; OTTHUMP00000235483; OTTHUMP00000235484; OTTHUMP00000235485; Polycomb protein eed; WAIT 1; WAIT-1; WAIT1; WD protein associating with integrin cytoplasmic tails 1; embryonic ectoderm development

Clonality

Polyclonal

Species Reactivity

Human, Rat

Form/Format

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purity/Purification

Immunogen Affinity Purified

Preparation and Storage

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

45,524 Da

OMIM

605984

3D Structure

ModBase 3D Structure for O75530

Immunogen

E Coli-derived human EED recombinant protein (Position: M256-R441). Human EED shares 100% amino acid (aa) sequence identity with mouse EED.

Ig Type

Rabbit IgG

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-EED antibody

Western Blot (WB)

Application Notes for anti-EED antibody

Western Blot Concentration: 0.1-0.5ug/ml

Product References and Citations for anti-EED antibody

1. "Entrez Gene: EED embryonic ectoderm development". 2. Rietzler M, Bittner M, Kolanus W, Schuster A, Holzmann B (Nov 1998). "The human WD repeat protein WAIT-1 specifically interacts with the cytoplasmic tails of beta7-integrins". J. Biol. Chem. 273 (42): 27459-66. 3. Schumacher A, Lichtarge O, Schwartz S, Magnuson T (Jan 1999). "The murine Polycomb-group gene eed and its human orthologue: functional implications of evolutionary conservation". Genomics 54(1): 79-88.

Other Notes

Small volumes of anti-EED antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EED purchase

MBS177817 is an antibody targeting EED. Reactive in Human, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for EED. It may not necessarily be applicable to this product.

NCBI GI #

808175997

NCBI GeneID

8726

NCBI Accession #

NP_001294936.1

UniProt Primary Accession #

O75530

UniProt Secondary Accession #

O00149; Q6NTH2; Q7LDA5; Q7LDG8; Q86VV2; Q9UNY7; A8K7V5

UniProt Related Accession #

O75530

GenBank Nucleotide #

NM_001308007.1

NCBI Official Full Name

polycomb protein EED isoform c

NCBI Official Synonym Full Names

embryonic ectoderm development

NCBI Official Symbol

EED

NCBI Official Synonym Symbols

HEED; WAIT1

UniProt Protein Name

Polycomb protein EED

UniProt Synonym Protein Names

WD protein associating with integrin cytoplasmic tails 1; WAIT-1

Protein Family

Polycomb protein

UniProt Gene Name

EED

UniProt Synonym Gene Names

hEED; WAIT-1

UniProt Entry Name

EED_HUMAN

NCBI Protein Information

polycomb protein EED

NCBI Summary for EED

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for EED

Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.

Research Articles on EED

1. An integral role for EED as an epigenetic exchange factor coordinating the activities of PRC1 and 2, is reported.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EED antibody

Reference Product	PubMed Publications
EZH2 antibody	>179 publications with EED and EZH2
SUZ12 antibody	>118 publications with EED and SUZ12
BMI1 antibody	>28 publications with EED and BMI1
EZH1 antibody	>20 publications with EED and EZH1
RNF2 antibody	>12 publications with EED and RNF2
HDAC1 antibody	>5 publications with EED and HDAC1
RBBP4 antibody	>4 publications with EED and RBBP4
AEBP2 antibody	>3 publications with EED and AEBP2
HDAC2 antibody	>3 publications with EED and HDAC2
RBBP7 antibody	>2 publications with EED and RBBP7

Pathways associated with anti-EED antibody

Products by Pathway	Pathway Diagram
Activation Of HOX Genes During Differentiation Pathway antibodies	Activation Of HOX Genes During Differentiation Pathway Diagram
Activation Of Anterior HOX Genes In Hindbrain Development During Early Embryogenesis Pathway antibodies	Activation Of Anterior HOX Genes In Hindbrain Development During Early Embryogenesis Pathway Diagram
Cellular Senescence Pathway antibodies	Cellular Senescence Pathway Diagram
Cellular Responses To Stress Pathway antibodies	Cellular Responses To Stress Pathway Diagram
Chromatin Modifying Enzymes Pathway antibodies	Chromatin Modifying Enzymes Pathway Diagram
Chromatin Organization Pathway antibodies	Chromatin Organization Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Epigenetic Regulation Of Gene Expression Pathway antibodies	Epigenetic Regulation Of Gene Expression Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Oxidative Stress Induced Senescence Pathway antibodies	Oxidative Stress Induced Senescence Pathway Diagram

Diseases associated with anti-EED antibody

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>85 publications with EED and Cardiovascular Diseases
Heart Diseases Antibodies	>39 publications with EED and Heart Diseases
Inflammation Antibodies	>18 publications with EED and Inflammation
Lung Diseases Antibodies	>15 publications with EED and Lung Diseases
Hypertension Antibodies	>12 publications with EED and Hypertension
Prostatic Neoplasms Antibodies	>12 publications with EED and Prostatic Neoplasms
Breast Neoplasms Antibodies	>12 publications with EED and Breast Neoplasms
Pain Antibodies	>11 publications with EED and Pain
Lung Neoplasms Antibodies	>8 publications with EED and Lung Neoplasms
Liver Diseases Antibodies	>7 publications with EED and Liver Diseases

Organs/Tissues associated with anti-EED antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>79 publications with EED and Blood
Skin Antibodies	>45 publications with EED and Skin
Heart Antibodies	>45 publications with EED and Heart
Embryonic Tissue Antibodies	>29 publications with EED and Embryonic Tissue
Bone Antibodies	>24 publications with EED and Bone
Brain Antibodies	>23 publications with EED and Brain
Prostate Antibodies	>19 publications with EED and Prostate
Lung Antibodies	>18 publications with EED and Lung
Nerve Antibodies	>14 publications with EED and Nerve
Liver Antibodies	>14 publications with EED and Liver