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Osteocalcin (BGLAP) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178418

Rabbit IgG polyclonal antibody for Osteocalcin (BGLAP) detection. Background: Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein found in bone and dentin. In humans, the osteocalcin is encoded by… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Osteocalcin (BGLAP), Polyclonal Antibody
Full Product Name
Anti-Osteocalcin Antibody
Product Gene Name
Product Synonym Names
BGLAP; Bone Gla protein; ocn; OSTCN HUMAN; Osteocalcin; P02818; Osteocalcin; bone gamma-carboxyglutamate (gla) protein
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
10,963 Da
OMIM
112260
3D Structure
ModBase 3D Structure for P02818
Immunogen
E Coli-derived human Osteocalcin recombinant protein (Position: Y52-V100). Human Osteocalcin shares 69% and 80.9% amino acid (aa) sequence identity with mouse and rat Osteocalcin, respectively.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-BGLAP antibody
Immunohistochemistry (IHC) Paraffin
Application Notes for anti-BGLAP antibody
Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Human
Tested Species:In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.

Immunohistochemistry (IHC) of anti-BGLAP antibody

Osteocalcin was detected in paraffin-embedded sections of human osteosarcoma tissues using rabbit anti-Osteocalcin Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-BGLAP Polyclonal Antibody – Cat# MBS178418

Product References and Citations for anti-BGLAP antibody

1. Cancela L, Hsieh CL, Francke U, Price PA (September 1990). "Molecular structure, chromosome assignment, and promoter organization of the human matrix Gla protein gene". J. Biol. Chem. 265 (25): 15040-8.
2. Lee NK, Sowa H, Hinoi E, Ferron M, Ahn JD, Confavreux C, Dacquin R, Mee PJ, McKee MD, Jung DY, Zhang Z, Kim JK, Mauvais-Jarvis F, Ducy P, Karsenty G (August 2007). "Endocrine regulation of energy metabolism by the skeleton". Cell 130 (3): 456-69.
3. Puchacz E, Lian JB, Stein GS, Wozney J, Huebner K, Croce C (May 1989). "Chromosomal localization of the human osteocalcin gene". Endocrinology 124 (5): 2648-50.

Other Notes

Small volumes of anti-BGLAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BGLAP purchase

MBS178418 is an antibody targeting BGLAP. Also known as Anti-Osteocalcin Antibody. Reactive in Human
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BGLAP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
osteocalcin preproprotein
NCBI Official Synonym Full Names
bone gamma-carboxyglutamate protein
NCBI Official Symbol
BGLAP
NCBI Official Synonym Symbols
OC; BGP; OCN
UniProt Protein Name
Osteocalcin
UniProt Synonym Protein Names
Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein
Protein Family
UniProt Gene Name
BGLAP
UniProt Synonym Gene Names
BGP
UniProt Entry Name
OSTCN_HUMAN
NCBI Protein Information
osteocalcin

NCBI Summary for BGLAP

This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]
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UniProt Comments for BGLAP

osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family.

Protein type: Cell development/differentiation; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: cytoplasm; endoplasmic reticulum lumen; Golgi lumen

Biological Process: ER to Golgi vesicle-mediated transport; osteoblast differentiation; peptidyl-glutamic acid carboxylation; response to vitamin D; signal peptide processing; skeletal development
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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