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ARF6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178487

Rabbit IgG polyclonal antibody for ADP-ribosylation factor 6 (ARF6) detection. Background: ADP-ribosylation factor 6 (ARF6) is a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding… More details

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Product Overview

Online Datasheet in PDF
Product Name
ARF6, Polyclonal Antibody
Full Product Name
Anti-ARF6 Antibody
Product Gene Name
anti-ARF6 antibody
Product Synonym Names
ADP ribosylation factor 6; ARF6; ARF 6; Small GTP binding protein; Small GTPase; P62330; ADP-ribosylation factor 6; ADP ribosylation factor 6
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
20,082 Da
OMIM
600464
3D Structure
ModBase 3D Structure for P62330
Immunogen
E Coli-derived human ARF6 recombinant protein (Position: G2-S175). Human ARF6 shares 100% amino acid (aa) sequence identity with both mouse and rat ARF6.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ARF6 antibody
Western Blot (WB)
Application Notes for anti-ARF6 antibody
Western Blot: 0.1-0.5mug/ml; Tested Species: Human, Mouse, Rat
Tested Species:In-house tested species with positive results.
Other applications have not been tested.

Western Blot (WB) of anti-ARF6 antibody

Western blot analysis of ARF6 expression in rat kidney extract (lane 1), mouse kidney extract (lane 2) and MCF-7 whole cell lysates (lane 3). ARF6 at 20KD was detected using rabbit anti- ARF6 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-ARF6 Polyclonal Antibody – Cat# MBS178487

Product References and Citations for anti-ARF6 antibody

1. "Entrez Gene: ARF6 ADP-ribosylation factor 6".
2. Prigent M, Dubois T, Raposo G, Derrien V, Tenza D, Rossé C, Camonis J, Chavrier P (December 2003). "ARF6 controls post-endocytic recycling through its downstream exocyst complex effector". J. Cell Biol. 163(5): 1111-21.
3. Peru y Colón de Portugal RL, Acevedo SF, Rodan AR, Chang LY, Eaton BA, Rothenfluh A (December 2012)."Adult neuronal Arf6 controls ethanol-induced behavior with Arfaptin downstream of Rac1 and RhoGAP18B".J. Neurosci. 32 (49): 17706-13.

Other Notes

Small volumes of anti-ARF6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ARF6 purchase

MBS178487 is an antibody targeting ARF6. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ARF6. It may not necessarily be applicable to this product.

NCBI GI #
4502211
NCBI GeneID
382
NCBI Accession #
NP_001654.1
UniProt Primary Accession #
P62330
UniProt Secondary Accession #
P26438; Q6FGZ2
UniProt Related Accession #
P62330
GenBank Nucleotide #
NM_001663.3
NCBI Official Full Name
ADP-ribosylation factor 6
NCBI Official Synonym Full Names
ADP ribosylation factor 6
NCBI Official Symbol
ARF6
UniProt Protein Name
ADP-ribosylation factor 6
Protein Family
ADP-ribosylation factor
UniProt Gene Name
ARF6
UniProt Entry Name
ARF6_HUMAN
NCBI Protein Information
ADP-ribosylation factor 6

NCBI Summary for ARF6

This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008]
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UniProt Comments for ARF6

ARF6: GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development. Contributes to the regulation of dendritic branching and filopodia extension. Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA. Interacts with NCS1/FREQ at the plasma membrane. Interacts with RAB11FIP3 and RAB11FIP4. Interacts with USP6 (via Rab-GAP TBC domain). Interacts with ECM29. Interacts with TBC1D24. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein, monomeric, ARF; G protein, monomeric; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q21.3

Cellular Component: cell cortex; endocytic vesicle; endosome; filopodium membrane; focal adhesion; membrane; plasma membrane; recycling endosome membrane; ruffle

Molecular Function: GTP binding; GTPase activity; protein binding; thioesterase binding

Biological Process: cell adhesion; cell motility; cortical actin cytoskeleton organization and biogenesis; negative regulation of receptor-mediated endocytosis; positive regulation of actin filament polymerization; regulation of filopodium formation; regulation of Rac protein signal transduction; ruffle organization and biogenesis; vesicle-mediated transport
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Research Articles on ARF6

1. The role of EGFR-ARF6 signaling in BA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ARF6 antibody

 Reference Product  PubMed Publications
 RAC1 antibody  >88 publications with ARF6 and RAC1
 ACAP1 antibody  >14 publications with ARF6 and ACAP1
 GGA3 antibody  >9 publications with ARF6 and GGA3
 SPAG9 antibody  >5 publications with ARF6 and SPAG9
 PXN antibody  >4 publications with ARF6 and PXN
 CYTH2 antibody  >4 publications with ARF6 and CYTH2
 TIAM1 antibody  >3 publications with ARF6 and TIAM1
 RAB11FIP3 antibody  >1 publications with ARF6 and RAB11FIP3
 CYTH3 antibody  >1 publications with ARF6 and CYTH3

Pathways associated with anti-ARF6 antibody

 Products by Pathway  Pathway Diagram
 Arf6 Downstream Pathway antibodies  Arf6 Downstream Pathway Diagram
 Arf6 Signaling Events Pathway antibodies  Arf6 Signaling Events Pathway Diagram
 Arf6 Trafficking Events Pathway antibodies  Arf6 Trafficking Events Pathway Diagram
 Class I PI3K Signaling Events Pathway antibodies  Class I PI3K Signaling Events Pathway Diagram
 E-cadherin Signaling In The Nascent Adherens Junction Pathway antibodies  E-cadherin Signaling In The Nascent Adherens Junction Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram

Diseases associated with anti-ARF6 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >7 publications with ARF6 and Inflammation
 Lung Neoplasms Antibodies  >7 publications with ARF6 and Lung Neoplasms
 Disease Models, Animal Antibodies  >6 publications with ARF6 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >6 publications with ARF6 and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >6 publications with ARF6 and Cardiovascular Diseases
 Cell Transformation, Neoplastic Antibodies  >6 publications with ARF6 and Cell Transformation, Neoplastic
 Liver Diseases Antibodies  >5 publications with ARF6 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >4 publications with ARF6 and Carcinoma, Hepatocellular
 Kidney Diseases Antibodies  >3 publications with ARF6 and Kidney Diseases
 Congenital Abnormalities Antibodies  >3 publications with ARF6 and Congenital Abnormalities

Organs/Tissues associated with anti-ARF6 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >73 publications with ARF6 and Blood
 Brain Antibodies  >72 publications with ARF6 and Brain
 Nerve Antibodies  >62 publications with ARF6 and Nerve
 Kidney Antibodies  >44 publications with ARF6 and Kidney
 Lung Antibodies  >35 publications with ARF6 and Lung
 Heart Antibodies  >31 publications with ARF6 and Heart
 Muscle Antibodies  >28 publications with ARF6 and Muscle
 Vascular Antibodies  >28 publications with ARF6 and Vascular
 Embryonic Tissue Antibodies  >16 publications with ARF6 and Embryonic Tissue
 Liver Antibodies  >14 publications with ARF6 and Liver
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