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CYBB Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179267

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the… More details

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Product Overview

Online Datasheet in PDF
Product Name
CYBB/Nox2, Monoclonal Antibody
Full Product Name
Anti-CYBB/Nox2 Rabbit Monoclonal Antibody
Product Gene Name
anti-CYBB antibody
Product Synonym Names
Cytochrome b-245 heavy chain; 1.-.-.-; CGD91-phox; Cytochrome b(558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; gp91-1; gp91-phox; p22 phagocyte B-cytochrome; CYBB; NOX2
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
65336 MW
OMIM
X04011 mRNA
3D Structure
ModBase 3D Structure for P04839
Immunogen
A synthesized peptide derived from human CYBB
Subcellular Localization
Cell membrane; Multi-pass membrane protein.
Tissue Specificity
Detected in neutrophils (at protein level).
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-CYBB antibody

Immunology; Adaptive Immunity; T Cells; cytotoxic cells; Cancer; Cancer Metabolism; Cellular Metabolic Process; Metabolism; Types of Disease
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CYBB antibody
Western Blot (WB)
Application Notes for anti-CYBB antibody
WB: 1:1000-1:2000

Western Blot (WB) of anti-CYBB antibody

Western blot analysis of CYBB expression in MCF-7 cell lysate (MBS179267).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYBB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYBB
Rabbit Anti-CYBB Monoclonal Antibody – Cat# MBS179267

Other Notes

Small volumes of anti-CYBB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CYBB purchase

MBS179267 is an antibody targeting CYBB. Also known as Anti-CYBB/Nox2 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CYBB. It may not necessarily be applicable to this product.

NCBI GI #
6996021
NCBI GeneID
1536
NCBI Accession #
NP_000388.2
UniProt Primary Accession #
P04839
UniProt Secondary Accession #
Q2PP16; A8K138
UniProt Related Accession #
P04839
GenBank Nucleotide #
NM_000397.3
NCBI Official Full Name
cytochrome b-245 heavy chain
NCBI Official Synonym Full Names
cytochrome b-245 beta chain
NCBI Official Symbol
CYBB
NCBI Official Synonym Symbols
CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
UniProt Protein Name
Cytochrome b-245 heavy chain
UniProt Synonym Protein Names
CGD91-phox; Cytochrome b(558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; gp91-1; gp91-phox; p22 phagocyte B-cytochrome
Protein Family
Cytochrome
UniProt Gene Name
CYBB
UniProt Synonym Gene Names
NOX2; Cytochrome b558 subunit beta
NCBI Protein Information
cytochrome b-245 heavy chain

NCBI Summary for CYBB

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]
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UniProt Comments for CYBB

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
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Research Articles on CYBB

1. Nox2 activity efficiently contributes to the mechanism of oxidative stress-induced increase in premature aging conferred by doxorubicin. The importance of modulation of Nox2 in human EPCs could reveal a useful tool to restore endothelial progenitor cells physiological function and properties.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CYBB antibody

 Reference Product  PubMed Publications
 CYBA antibody  >119 publications with CYBB and CYBA
 RAC1 antibody  >83 publications with CYBB and RAC1
 NCF1 antibody  >40 publications with CYBB and NCF1
 NCF2 antibody  >31 publications with CYBB and NCF2
 NCF4 antibody  >9 publications with CYBB and NCF4
 MMP9 antibody  >4 publications with CYBB and MMP9
 VCAM1 antibody  >1 publications with CYBB and VCAM1

Pathways associated with anti-CYBB antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing-Cross Presentation Pathway antibodies  Antigen Processing-Cross Presentation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway antibodies  Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway antibodies  Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram

Diseases associated with anti-CYBB antibody

 Disease Name  Pubmed Publications
 Granulomatous Disease, Chronic Antibodies  >298 publications with CYBB and Granulomatous Disease, Chronic
 Cardiovascular Diseases Antibodies  >288 publications with CYBB and Cardiovascular Diseases
 Inflammation Antibodies  >225 publications with CYBB and Inflammation
 Disease Models, Animal Antibodies  >172 publications with CYBB and Disease Models, Animal
 Heart Diseases Antibodies  >118 publications with CYBB and Heart Diseases
 Hypertension Antibodies  >117 publications with CYBB and Hypertension
 Necrosis Antibodies  >107 publications with CYBB and Necrosis
 Reperfusion Injury Antibodies  >61 publications with CYBB and Reperfusion Injury
 Fibrosis Antibodies  >57 publications with CYBB and Fibrosis
 Atherosclerosis Antibodies  >56 publications with CYBB and Atherosclerosis

Organs/Tissues associated with anti-CYBB antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >608 publications with CYBB and Blood
 Vascular Antibodies  >324 publications with CYBB and Vascular
 Brain Antibodies  >119 publications with CYBB and Brain
 Lung Antibodies  >110 publications with CYBB and Lung
 Kidney Antibodies  >92 publications with CYBB and Kidney
 Bone Antibodies  >78 publications with CYBB and Bone
 Bone Marrow Antibodies  >71 publications with CYBB and Bone Marrow
 Liver Antibodies  >44 publications with CYBB and Liver
 Eye Antibodies  >25 publications with CYBB and Eye
 Connective Tissue Antibodies  >21 publications with CYBB and Connective Tissue
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