• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

iNOS Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179289

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
iNOS, Monoclonal Antibody
Full Product Name
Anti-iNOS Rabbit Monoclonal Antibody
Product Gene Name
anti-iNOS antibody
Product Synonym Gene Name
NOS2
Product Synonym Names
Nitric oxide synthase; inducible; 1.14.13.39; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; N
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
131117 MW
OMIM
L24553 mRNA
3D Structure
ModBase 3D Structure for P35228
Immunogen
A synthesized peptide derived from human iNOS
Tissue Specificity
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-iNOS antibody

Neuroscience; Neurotransmission; Nitric Oxide; NOS; Cancer; Cancer Metabolism; Response to Hypoxia; Cellular Metabolic Process; Metabolism; Pathways and Processes; Metabolism Processes; Types of Disease
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-iNOS antibody
Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-iNOS antibody
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IP: 1:50

Western Blot (WB) of anti-iNOS antibody

Western blot analysis of iNOS expression in Human fetal brain lysate (MBS179289).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOS2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NOS2
Rabbit Anti-iNOS Monoclonal Antibody – Cat# MBS179289

Other Notes

Small volumes of anti-iNOS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for iNOS purchase

MBS179289 is an antibody targeting iNOS. Also known as Anti-iNOS Rabbit Monoclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for iNOS. It may not necessarily be applicable to this product.

NCBI GI #
24041029
NCBI GeneID
4843
NCBI Accession #
NP_000616.3
UniProt Primary Accession #
P35228
UniProt Secondary Accession #
O60757; O94994; Q16263; Q16692; Q4TTS5; Q9UD42; A1L3U5; B7ZLY2
UniProt Related Accession #
P35228
GenBank Nucleotide #
NM_000625.4
NCBI Official Full Name
nitric oxide synthase, inducible
NCBI Official Synonym Full Names
nitric oxide synthase 2
NCBI Official Symbol
NOS2
NCBI Official Synonym Symbols
NOS; INOS; NOS2A; HEP-NOS
UniProt Protein Name
Nitric oxide synthase, inducible
UniProt Synonym Protein Names
Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2
Protein Family
Inosamine-phosphate amidinotransferase
UniProt Gene Name
NOS2
UniProt Synonym Gene Names
NOS2A; HEP-NOS; Inducible NOS; iNOS
NCBI Protein Information
nitric oxide synthase, inducible

NCBI Summary for iNOS

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for iNOS

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109).
Show more

Research Articles on iNOS

1. Bone marrow mesenchymal stromal cells induce the rapid differentiation of CD11b+ myeloid cells from bone marrow progenitors, and such an activity requires the expression of nitric oxide synthase-2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-iNOS antibody

 Reference Product  PubMed Publications
 SRC antibody  >34 publications with iNOS and SRC
 RAC1 antibody  >12 publications with iNOS and RAC1
 CAV1 antibody  >4 publications with iNOS and CAV1
 RAC2 antibody  >2 publications with iNOS and RAC2
 SLC9A3R1 antibody  >1 publications with iNOS and SLC9A3R1
 ACTN4 antibody  >1 publications with iNOS and ACTN4

Pathways associated with anti-iNOS antibody

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram

Diseases associated with anti-iNOS antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2190 publications with iNOS and Inflammation
 Necrosis Antibodies  >1844 publications with iNOS and Necrosis
 Sepsis Antibodies  >542 publications with iNOS and Sepsis
 Intestinal Diseases Antibodies  >370 publications with iNOS and Intestinal Diseases
 Liver Diseases Antibodies  >346 publications with iNOS and Liver Diseases
 Reperfusion Injury Antibodies  >346 publications with iNOS and Reperfusion Injury
 Hypertension Antibodies  >280 publications with iNOS and Hypertension
 Endotoxemia Antibodies  >248 publications with iNOS and Endotoxemia
 Shock, Septic Antibodies  >235 publications with iNOS and Shock, Septic
 Kidney Diseases Antibodies  >211 publications with iNOS and Kidney Diseases

Organs/Tissues associated with anti-iNOS antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2501 publications with iNOS and Blood
 Brain Antibodies  >1050 publications with iNOS and Brain
 Lung Antibodies  >810 publications with iNOS and Lung
 Intestine Antibodies  >451 publications with iNOS and Intestine
 Pancreas Antibodies  >211 publications with iNOS and Pancreas
 Stomach Antibodies  >208 publications with iNOS and Stomach
 Embryonic Tissue Antibodies  >88 publications with iNOS and Embryonic Tissue
 Connective Tissue Antibodies  >87 publications with iNOS and Connective Tissue
 Mixed Antibodies  >71 publications with iNOS and Mixed
 Ovary Antibodies  >45 publications with iNOS and Ovary
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000