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Survivin (BIRC5) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179298

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an… More details

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Product Overview

Online Datasheet in PDF
Product Name
Survivin (Birc5), Monoclonal Antibody
Full Product Name
Anti-Survivin Rabbit Monoclonal Antibody
Product Gene Name
anti-Birc5 antibody
Product Synonym Names
Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; TIAP; Birc5; Api4; Iap4
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
16298 MW
3D Structure
ModBase 3D Structure for O70201
Immunogen
A synthesized peptide derived from human Survivin
Subcellular Localization
Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export (By similarity).
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-Birc5 antibody

Cell Biology; Apoptosis; Intracellular; Survivin/IAPS; Epigenetics and Nuclear Signaling; Chromosome Structure; Chromosome; Scaffold Proteins; Cell Cycle; Nuclear; Transcription; Transcription Factors; Cell Biology; Cell Division; Other Cell Division Anti
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Birc5 antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-Birc5 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IP: 1:30

Western Blot (WB) of anti-Birc5 antibody

Western blot analysis of Survivin expression in Neuro-2a cell lysate (MBS179298).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Birc5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Birc5
Rabbit Anti-BIRC5 Monoclonal Antibody – Cat# MBS179298

Immunohistochemistry (IHC) of anti-Birc5 antibody

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using Survivin Antibody(MBS179298)
Birc5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Birc5 Antibody (MBS179298)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-BIRC5 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS179298

Other Notes

Small volumes of anti-Birc5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Birc5 purchase

MBS179298 is an antibody targeting Birc5. Also known as Anti-Survivin Rabbit Monoclonal Antibody. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Birc5. It may not necessarily be applicable to this product.

NCBI GI #
59859890
NCBI GeneID
11799
NCBI Accession #
NP_001012273.1
UniProt Primary Accession #
O70201
UniProt Secondary Accession #
Q923F7; Q9WU53; Q9WU54
GenBank Nucleotide #
NP_001012273.1
NCBI Official Full Name
baculoviral IAP repeat-containing protein 5 isoform 3
UniProt Protein Name
Baculoviral IAP repeat-containing protein 5
UniProt Synonym Protein Names
Apoptosis inhibitor 4; Apoptosis inhibitor survivin; TIAP
Protein Family
Baculoviral IAP repeat-containing protein
UniProt Gene Name
Birc5
UniProt Synonym Gene Names
Api4; Iap4

UniProt Comments for Birc5

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Birc5 antibody

 Reference Product  PubMed Publications
 Casp9 antibody  >126 publications with Birc5 and Casp9
 Aurkb antibody  >87 publications with Birc5 and Aurkb
 Incenp antibody  >54 publications with Birc5 and Incenp
 Cdk1 antibody  >36 publications with Birc5 and Cdk1
 Aurka antibody  >30 publications with Birc5 and Aurka
 Cdca8 antibody  >30 publications with Birc5 and Cdca8
 Plk1 antibody  >29 publications with Birc5 and Plk1
 Bub1 antibody  >16 publications with Birc5 and Bub1
 Mad2l1 antibody  >10 publications with Birc5 and Mad2l1
 Kif2c antibody  >8 publications with Birc5 and Kif2c

Pathways associated with anti-Birc5 antibody

 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis - Multiple Species Pathway antibodies  Apoptosis - Multiple Species Pathway Diagram
 Apoptosis - Multiple Species Pathway antibodies  Apoptosis - Multiple Species Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Colorectal Cancer Pathway antibodies  Colorectal Cancer Pathway Diagram
 Colorectal Cancer Pathway antibodies  Colorectal Cancer Pathway Diagram
 Hepatitis B Pathway antibodies  Hepatitis B Pathway Diagram

Diseases associated with anti-Birc5 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >518 publications with Birc5 and Adenocarcinoma
 Breast Neoplasms Antibodies  >359 publications with Birc5 and Breast Neoplasms
 Lung Neoplasms Antibodies  >324 publications with Birc5 and Lung Neoplasms
 Neoplasm Metastasis Antibodies  >310 publications with Birc5 and Neoplasm Metastasis
 Carcinoma, Small Cell Antibodies  >293 publications with Birc5 and Carcinoma, Small Cell
 Colorectal Neoplasms Antibodies  >282 publications with Birc5 and Colorectal Neoplasms
 Liver Neoplasms Antibodies  >233 publications with Birc5 and Liver Neoplasms
 Liver Diseases Antibodies  >217 publications with Birc5 and Liver Diseases
 Neoplasms, Experimental Antibodies  >204 publications with Birc5 and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >202 publications with Birc5 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-Birc5 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >390 publications with Birc5 and Lung
 Liver Antibodies  >273 publications with Birc5 and Liver
 Lymph Node Antibodies  >209 publications with Birc5 and Lymph Node
 Brain Antibodies  >172 publications with Birc5 and Brain
 Stomach Antibodies  >164 publications with Birc5 and Stomach
 Bone Antibodies  >153 publications with Birc5 and Bone
 Kidney Antibodies  >100 publications with Birc5 and Kidney
 Bone Marrow Antibodies  >99 publications with Birc5 and Bone Marrow
 Muscle Antibodies  >91 publications with Birc5 and Muscle
 Embryonic Tissue Antibodies  >73 publications with Birc5 and Embryonic Tissue
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