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ATG16L1 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179352

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the… More details

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Product Overview

Online Datasheet in PDF
Product Name
Atg16L1/Atg16, Monoclonal Antibody
Full Product Name
Anti-Atg16L1/Atg16 Rabbit Monoclonal Antibody
Product Gene Name
anti-ATG16L1 antibody
Product Synonym Names
Autophagy-related protein 16-1; APG16-like 1; ATG16L1; APG16L; UNQ9393/PRO34307
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
68265 MW
OMIM
AY398617 mRNA
3D Structure
ModBase 3D Structure for Q676U5
Immunogen
A synthesized peptide derived from human Atg16L1
Subcellular Localization
Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-ATG16L1 antibody

Cell Biology; Proteolysis/Ubiquitin; Proteasome/Ubiquitin; UB-Like Proteins; Neuroscience; Neurology Process; Neurodegenerative Disease; Cancer; Oncoproteins/Suppressors; Tumor Suppressors; Cardiovascular; Heart; Autophagy; APG Gene Products; Signal Trans
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ATG16L1 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-ATG16L1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Western Blot (WB) of anti-ATG16L1 antibody

Western blot analysis of Atg16L1 expression in HeLa cell lysate (MBS179352).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG16L1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATG16L1
Rabbit Anti-ATG16L1 Monoclonal Antibody – Cat# MBS179352

Other Notes

Small volumes of anti-ATG16L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG16L1 purchase

MBS179352 is an antibody targeting ATG16L1. Also known as Anti-Atg16L1/Atg16 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG16L1. It may not necessarily be applicable to this product.

NCBI GI #
298229005
NCBI GeneID
55054
NCBI Accession #
NP_001177195.1
UniProt Primary Accession #
Q676U5
UniProt Secondary Accession #
Q6IPN1; Q6UXW4; Q6ZVZ5; Q8NCY2; Q96JV5; Q9H619; A3EXK9; A3EXL0; B6ZDH0
UniProt Related Accession #
Q676U5
GenBank Nucleotide #
NM_001190266.1
NCBI Official Full Name
autophagy-related protein 16-1 isoform 4
NCBI Official Synonym Full Names
autophagy related 16 like 1
NCBI Official Symbol
ATG16L1
NCBI Official Synonym Symbols
IBD10; WDR30; APG16L; ATG16A; ATG16L
UniProt Protein Name
Autophagy-related protein 16-1
UniProt Synonym Protein Names
APG16-like 1
Protein Family
Autophagy-related protein
UniProt Gene Name
ATG16L1
UniProt Synonym Gene Names
APG16L
NCBI Protein Information
autophagy-related protein 16-1

NCBI Summary for ATG16L1

The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010]
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UniProt Comments for ATG16L1

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:24553140, PubMed:23376921, PubMed:24954904, PubMed:27273576, PubMed:23392225). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (PubMed:25645662). Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response (PubMed:24238340). Plays a role in regulating morphology and function of Paneth cell (PubMed:18849966).
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Research Articles on ATG16L1

1. transient expression of ATG16L1 not only inhibited autophagosome biogenesis, but also aberrantly targeted RAB11-positive recycling endosomes, resulting in recycling endosome aggregates.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ATG16L1 antibody

 Reference Product  PubMed Publications
 ATG5 antibody  >26 publications with ATG16L1 and ATG5

Pathways associated with anti-ATG16L1 antibody

 Products by Pathway  Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram

Diseases associated with anti-ATG16L1 antibody

 Disease Name  Pubmed Publications
 Crohn Disease Antibodies  >130 publications with ATG16L1 and Crohn Disease
 Inflammation Antibodies  >46 publications with ATG16L1 and Inflammation
 Inflammatory Bowel Disease 10 Antibodies  >36 publications with ATG16L1 and Inflammatory Bowel Disease 10
 Necrosis Antibodies  >16 publications with ATG16L1 and Necrosis
 Neoplasms Antibodies  >6 publications with ATG16L1 and Neoplasms
 Disease Models, Animal Antibodies  >6 publications with ATG16L1 and Disease Models, Animal
 Death Antibodies  >3 publications with ATG16L1 and Death
 Liver Diseases Antibodies  >3 publications with ATG16L1 and Liver Diseases
 Carcinoma Antibodies  >3 publications with ATG16L1 and Carcinoma
 Nervous System Diseases Antibodies  >3 publications with ATG16L1 and Nervous System Diseases

Organs/Tissues associated with anti-ATG16L1 antibody

 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >35 publications with ATG16L1 and Intestine
 Blood Antibodies  >21 publications with ATG16L1 and Blood
 Brain Antibodies  >6 publications with ATG16L1 and Brain
 Liver Antibodies  >4 publications with ATG16L1 and Liver
 Nerve Antibodies  >4 publications with ATG16L1 and Nerve
 Bone Marrow Antibodies  >3 publications with ATG16L1 and Bone Marrow
 Bone Antibodies  >3 publications with ATG16L1 and Bone
 Heart Antibodies  >3 publications with ATG16L1 and Heart
 Muscle Antibodies  >1 publications with ATG16L1 and Muscle
 Thymus Antibodies  >1 publications with ATG16L1 and Thymus
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